Piotr Kolata

Correlative MS Imaging for cellular identification and analysis of in situ cryo-ET www.biorxiv.org/content/10.1101/2025.09.16.676641v1 #cryoEM

The advent of confocal laser scanning microscopy in biological research Nature Reviews Molecular Cell Biology, Published online: 01 October 2025; doi:10.1038/s41580-025-00905-5The advent of laser-scanning confocal microscopy revolutionized cell biology, offering unprecedented resolution and depth and enabling scientists to visualize cellular structures in 3D.

New Online! The advent of confocal laser scanning microscopy in biological research

Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching www.biorxiv.org/content/10.1101/2025.09.11.675606v1 #cryoEM

This one is a bit of a departure from the usual and definitely a work in progress!

We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.

Read on for details... 1/x

PickET: An unsupervised method for localizing macromolecules in cryo-electron tomograms pubmed.ncbi.nlm.nih.gov/40894555/ #cryoEM

Now published @cp-cellreports.bsky.social! Antibody-mediated TGF-β1 activation for the treatment of diseases caused by deleterious T cell activity: www.cell.com/cell-reports...
Fantastic collaboration & massive effort from Sophie Lucas Lab @ de Duve Institute. #CryoEM with @savvideslab.bsky.social.

Upper - cryo-EM reconstruction of stomatin AQP1 complex. Lower - reconstruction of stomatin-UT-B complex.

Happy to share a new preprint from the Clarke & Vallese (@fravallese.bsky.social) labs reporting #cryoem structures of two RBC stomatin complexes – with AQP1 & UT-B - continuing the SPFH theme from our recent vault preprint! This was a fun one and has been cooking for a while - read on for more! ⬇️

EFFORTOCRACY Exploring the concept of Effortocracy, a society where rewards reflect effort, contrasting it with traditional meritocracy based on achievements.

Lucky for you I've coined yet another term... now before you get too excited, it is about meritocracy. The first simul-post on both the website: nonzerosum.games/effortocracy... and the podcast: podcasts.apple.com/nz/podcast/n... Choose whichever is least painful. #Meritocracy #Society #Policy #DEI

Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators.

www.nature.com/articles/s41...

The small GTPase Ran defines nuclear pore complex asymmetry Nuclear pore complexes (NPCs) bridge across the nuclear envelope and mediate nucleocytoplasmic exchange. They consist of hundreds of nucleoporin build…

My PhD paper is in press @cellpress.bsky.social and now online: www.sciencedirect.com/science/arti...!

Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more!

Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo

Cryo-EM identifies F-ENA of Bacillus thuringiensis as a widespread family of endospore appendages across Firmicutes - Nature Communications B. thuringiensis spores contain uncharacterized protein filaments that extend from the surface of the exosporium. Here, the authors show that these filaments feature conserved β-barrel neck domains an...

Many spores from the Firmicutes group are decorated with hair-like structures. Since the 60s, they remained a mystery. @remaut-lab.bsky.social named these fibres ENdospore Appendages (ENAs). Using Cryo-ID, we report the identity, structure and function of F-ENA.
www.nature.com/articles/s41...

We’re looking for a Research Support Officer to join @matteoall.bsky.social’s group, working with @piotrkolata.bsky.social to investigate nuclear-cytoskeletal coupling in mammalian cells using #cryoEM, #cryoET & other biochemical approaches.
www.jobs.ac.uk/job/DOE317/r...
Closes 31 AUG
#ScienceJobs

We are excited to announce the first in-cell structure of a LINC complex within a native nuclear envelope at subnanometer resolution!! #In-cell cryo-ET + #atomistic MD simulations! @tomdendooven.bsky.social et al!!
www.biorxiv.org/content/10.1...

Check out our latest work now out on biorxiv www.biorxiv.org/content/10.1...

Congrats Kashish & everyone involved! Awesome stuff!

Attendees of the recent bacteriorhopsin symposium, photographed from above.

Balsawood model of bacteriorhodopsin

Black and white photograph of Nigel Unwin and Richard Henderson sitting outside the LMB, 1991

Symposium attendees applauding in the LMB's Max Perutz Lecture Theatre.

The LMB recently hosted a symposium to mark 50 years since Richard Henderson and Nigel Unwin published the structure of bacteriorhodopsin, the first 3D structure of a protein determined by #cryoEM.

Read about the event and find more photos here: www2.mrc-lmb.cam.ac.uk/the-structur...

#LMBNews 🧪🔬

In-cell structure of a LINC complex reveals the molecular basis for membrane remodelling and head-to-tail coupling in sperm cells In eukaryotic cells, LINC complexes physically bridge the two nuclear membranes to cytoskeletal filaments, transmitting mechanical forces to the nucleus. Due to their dynamic and membrane-embedded nat...

Fantastic work by the group of @matteoall.bsky.social : a SUN5:Nesprin3 complex at the head:tail junction of sperm cells. 🤯 #teamtomo

www.biorxiv.org/content/10.1...

Dear all, we are looking for a research assistant in protein biochemistry / cell biology to support our work on nuclear reshaping in human cells. Here the link to the call, please spread: mrc.tal.net/vx/mobile-0/...

Sub-3 Å resolution protein structure determination by single-particle cryo-EM at 100 keV Cryoelectron microscopy (cryo-EM) has transformed structural biology by providing high-resolution insights into biological macromolecules. We report s…

Now out in Structure: Paper on high-resolution #cryoEM using the 100 kV Tundra cryo-TEM by Dimple Karia, Adrian Koh, @abhaykot.bsky.social, @lingboyu.bsky.social, @landerlab.bsky.social and many others. Happy to have contributed to this effort. Check it out:
www.sciencedirect.com/science/arti...

Cool amyloid structures! Congrats Braja and the team!

Happy to share our latest work on the structure and assembly of holocentric kinetochores! Huge thanks to Ines for a very fruitful collaboration, Claudio for all the support, and congratulations to Christine and all co-authors!

www.biorxiv.org/content/10.1...

Excited to share our latest work! We found transcription factor SPT6 and phosphorylated Pol II CTD help recruit U1 snRNP to elongating Pol II, allowing efficient co-transcriptional splicing.

Glad to be featured in the Editors’ Highlights!

www.nature.com/articles/s41...

#splicing #cryoEM

Cryo-EM structure of human telomerase dimer reveals H/ACA RNP-mediated dimerization Telomerase ribonucleoprotein (RNP) synthesizes telomeric repeats at chromosome ends using a telomerase reverse transcriptase (TERT) and a telomerase RNA (hTR in humans). Previous structural work showe...

Proud to share our work where we resolved a longstanding question regarding the existence of a human telomerase dimer and provided insights into its function. Led by 3 amazing lab members in collaboration with @yiliangding.bsky.social and @rdaslab.bsky.social.
www.science.org/doi/10.1126/...

Congratulations Jan & the team, very cool work!

A novel "bio-tag" for cryo-EM studies based on the small, electron-dense protein Csp1 pubmed.ncbi.nlm.nih.gov/40620219/ #cryoEM

Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation - Nature Structural & Molecular Biology Using cryo-electron microscopy and biochemistry, Zhang et al. reveal that the DNA helicase RECQL5 and the transcription-coupled DNA repair complex coordinate to regulate transcription elongation rates...

A transcription balance required for genome stability 🧬⚖️

Our work on RNA Pol II transcription speed control by the DNA helicase RECQL5 and the transcription-coupled DNA repair complex is now on @natsmb.nature.com

#CryoEM #transcription #genomestability

www.nature.com/articles/s41...

New cryo-EM lore just dropped ft Niko and lab
#SmallStackHighRes #2DTM #cryoEM
....

For educational purposes only, copyright: 300 (film), Warner Brothers

To tilt or not to tilt? Strategies for in situ cryo-EM data collection Recent breakthroughs in single-particle cryogenic electron microscopy (cryo-EM) and protein structure prediction have transformed our ability to resol…

Check out our recent review discussing the merits of tilt series vs single micrographs for in situ cryo-EM! www.sciencedirect.com/science/arti...

Running Phenix and stuck?
Now you can ask the new Phenix chatbot anything from the 600-page tutorial about how to run any program.
Big thanks to Tom Willinger for making this happen!
Try it out: phenix-online.org/version_docs...

Our new preprint revealing a new mechanism of mitochondrial disease pathophysiology: the accumulation of toxic complex I intermediates bound to complex III

www.biorxiv.org/content/10.1...