Canceled meetings and confusion: NIH grant funding in limbo despite court injunction Science researchers awaiting National Institutes of Health funding say their grant meetings are being canceled, despite a court order blocking the Trump administration from freezing federal funds.

"Stevenson hopes his work changes the drug treatment for Lyme disease. 'If these proposals never get reviewed, or if council meets too late, I won't have any money, and I’ll have to let people go,' he said. 'It’s very frustrating and very depressing.' ”

🦠🧫🧪🔬🧬

www.wbur.org/news/2025/02...

Sliding clamp protein enables long-range gene silencing in a bacterial plasmid - Nature Microbiology Long-distance gene regulation is uncommon in bacteria, and its molecular mechanisms are unclear. Using a combination of structural, biochemical and single-molecule techniques, researchers revealed tha...

Cool “Behind the paper” article on our recent KorB-KorA sliding clamp-locking clamp story. I remember Chris Thomas and Lewis Bingle (Birmingham) taught me about these proteins when I was an undergrad, and I kept remembering they said the mechanism was not clear!!!
www.nature.com/articles/s41...

Targeted decontamination of sequencing data with CLEAN https://www.biorxiv.org/content/10.1101/2023.08.05.552089v3 🧬🖥️🧪 Nextflow: https://github.com/rki-mf1/clean

JPM 2025 Events List by TechBio Transformers

JPM is happening in SF next week. This is an investor meeting but also traditionally a time for many biotech and health social events. The @techbio.bsky.social community has crowdsourced 30+ panels, dinners and parties for anyone interested docs.google.com/spreadsheets...

1/ 🔬 Our work, "Dynamic transitions of initiator binding coordinate the replication of the two chromosomes in Vibrio cholerae", is now published in Nature Communications.
Here's a thread on how we think Chr1 and Chr2 replication is coordinated in Vibrio. 🧵

Link : www.nature.com/articles/s41...

A virally encoded tRNA neutralizes the PARIS antiviral defence system - Nature Structural and functional studies reveal how viral proteins trigger the phage antirestriction induced system (PARIS) to degrade host tRNA and how viral tRNAs suppress the PARIS nuclease and thereby ov...

Today's holiday ☕ reading is this 2024 paper on PARIS, a bacterial defense system shaped like a literal propeller that responds to viral infection by hitting the cellular self-destruct button - cleaving an essential tRNA. www.nature.com/articles/s41...

The total length of human DNA on the planet right now is thus: (3.4x10^-10) • (2 x 3.055x10^9) • (3x10^13) • (8.2x10^9) = 5.11 x 10^23 meters

Time to call in NASA!

1 *light-year* – the distance light travels in one year – is 9.46x10^15 meters...

So: 54 million light-years of human DNA!

Hot Plasmids: Fall 2024 Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Love seeing what's new in the Addgene repository? So do we!

Check out our latest 🔥Hot Plasmids 🔥 post to see five Addgenie's picks for what's new and hot in our plasmid (and antibody!) repository this fall.

🧪🧬


blog.addgene.org/hot...
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Our new preprint is out - please share!

We asked: How can we make CRISPR knockouts in vivo more efficient? And set up an assay that enables detection of CRISPR cutting with 100s-1000s of sgRNAs over entire chromosome arms in living animals.

Grab a ☕ and let’s dive in. 🧵🧪 1/n
#CRISPR #geneediting

It's cool in the RNA modifications world systematic basecalling errors are one strategy to identify modified sites. Kind of a hacky (and I'm sure has false positives) approach but appealing in its simplicity.
It was also done for Phosphorothioated DNA here: www.frontiersin.org/journals/mic...

I’m sharing again the link to the Genomics Core Facilities starter pack that I put together as I added more accounts and feeds today go.bsky.app/HiWxZZc

They showed a plot at innovation day in NC that claimed 8x improvement in R2. I’ll see if I can find the slide.

Chromatin context-dependent regulation and epigenetic manipulation of prime editing Prime editing exhibits strong, chromatin-related position effects and is promoted by active transcriptional elongation. Leveraging these features via epigenetic conditioning of a locus modulates prime...

Chromatin context-dependent regulation and epigenetic manipulation of prime editing

www.cell.com/cell/fulltex...