NayimGR

⏪ G&D RECAP ⏪

🗓️ October 2025
RESEARCH COMMUNICATION: Structural basis of nucleosome recognition by the conserved Dsup & HMGN nucleosome-binding motif
By Alegrio-Louro et al. and Andres Leschziner
➡️ https://genesdev.cshlp.org/content/39/19-20/1155.full

#proteininteractions #proteinstructure #cryoEM

This one does github.com/hanjinliu/st...

That’s cool! Is it possible that your repo is private? The link doesn't work for me 🙄

It’s out! Using cryo-ET in Dicty cells, we take a fresh in situ look at vaults. Surprisingly, we uncover vaults associated with ER and NE membranes, and find that many vaults enclose ribosomes in defined orientations, opening new avenues to their cellular function! www.biorxiv.org/lookup/conte...

Did I just see a picture of a phage hijacking its host within the host of that host?!?!? Coolest thing ever.

Ten years of the CCP-EM Spring Symposium This article reviews the growth over the last ten years of the CCP-EM Spring Symposium. The conference is now established as a popular meeting for practitioners of life-science cryoEM and highlights new methods and exemplar applications.

Giles and Burnley: Ten years of the CCP-EM Spring Symposium #Cryoem #ElectronCryoMicroscopy #CCPEM ... #IUCr https://journals.iucr.org/paper?S2059798325010496

I'm still irrationally attached to Relion's GUI (old habits die hard?), but I'm having a great time running model refinement/validation with Doppio!

It's been a really good and fun read so far, congratulations :)

Doppio is a great tool (more of a toolbelt actually) to go all the way from micrographs to deposition!! (and the more people using it, the greater it gets)

Lab’s first paper is out!! We show the first structures of #Asgard #chromatin by #cryo-EM 🧬❄️
Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...

This one:

store.ioppublishing.org/page/detail/...

I think the SPA textbook by Nogales, Glaeser and Wah Chiu might work

I am excited to share our new preprint on the CAGE complex, a mysterious hollow protein complex that I first saw years ago while surveying Tetrahymena ciliary lysate www.biorxiv.org/content/10.1... #cilia #protistsonsky 🧬🧪

The structure of the complete extracellular bacterial flagellum reveals the mechanism of flagellin incorporation - Nature Microbiology Cryo-electron microscopy analysis of the extracellular flagellum structure, including the hook–filament junction and filament cap complex, reveals mechanisms of flagellin incorporation into the growin...

The peer-reviewed version of our article on the flagellum filament assembly is out!
A masterpiece from duo extraordinaire Kailin and Rosa, and an incredibly productive collaboration with Marc Erhardt and Morgan Beeby.

Cryo-EM structure of the vault from human brain reveals symmetry mismatch at its caps The vault protein is expressed in most eukaryotic cells, where it is assembled on polyribosomes into large hollow barrel-shaped complexes. Despite its widespread and abundant presence in cells, the bi...

The amazing Sofia Lövestam initiated the below project, when she became interested in the vault particles that we sometimes observe in #cryoEM images of brain-derived #amyloid filaments.
www.biorxiv.org/content/10.1...

🦠🧠 MemBrain update! 🧠🦠
We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics.
🔗 Preprint: doi.org/10.1101/2024...
🔗 Code: github.com/CellArchLab/...
🧵(1/6) #TeamTomo

If you process each of your dataset halves in different floors at Lumon you achieve *actual* gold-standard FSCs

Mature #HIV-1 contains >2000 copies of “spacer peptide 2”, but we didn’t know why. We now see that SP2 binds the matrix protein and triggers the change into its mature arrangement. Summary video from @margotriggi.bsky.social

REGISTRATION OPEN!! - CNIO meeting, Machines acting on DNA and RNA, 2025 May 28-30th in Madrid, organized by @nogaleslab.bsky.social , @rfleiro.bsky.social , and @oscarllorca.bsky.social

Interested in joining us? The Green Algorithms project promotes environmentally sustainable computational science through an online calculator, training material and other tools.

🚨We're recruiting! Interested in making science and computing more environmentally friendly? Now's the time!

Very excited to build a research group at Cambridge Uni to expand our Green Algorithms work thanks to the support of the Wellcome Trust
www.green-algorithms.org/join-us
#AcademicSky #SciSky

Missing the #FSCwars?

Here is a starter pack for CryoEM/ET enthousiasts
To be completed
go.bsky.app/JmQksC

Spilling the elutions of the first purification I got to work nicely while showing off that it was "so concentrated that is yellowish" to my labmates :')

Wouldn't mind to be added, and @rfleiro.bsky.social surely wouldn't either :) thanks!