André Mateus

Discovering Glycosylation-Dependent Protein Function by Thermal Proteome Profiling Protein glycosylation regulates essential cellular processes including protein folding, stability, and cell-cell interactions; however, how aberrant glycosylation impacts protein function and interact...

Congratulations former postdoc @jfhevler.bsky.social (in transit to Roche) and the team on their work using thermal proteome profiling along with glycosylation perturbagens to discover glycan-dependent protein functions at the proteome scale 👏

www.biorxiv.org/content/10.6...

The pcnB gene sustains Shigella flexneri virulence Author summary Bacterial infections represent a major global threat. Understanding the genetic determinants promoting infections is crucial to overcome this threat. Shigella is an intracellular bacter...

Thrilled to share that our study on how the pcnB gene sustains Shigella virulence is now published in PLOS Pathogens: journals.plos.org/plospathogen...

In short: pcnB ➝increased virulence-plasmid replication ➝ elevated T3SS expression ➝ enhanced invasion/spread within the intestinal epithelium.

Systematic screen uncovers regulator contributions to chemical cues in Escherichia coli Transport machineries are crucial for antibiotic resistance, but their regulation by external cues is poorly understood. This study identifies environmental chemicals that regulate transporter transcr...

Finally out! A small screen helps understand a little more about how E. coli regulates major transporters, and reveals how caffeine induces phenotypical antibiotic resistance. Huge thanks to collab @andrenmateus.bsky.social (Umeå) and Christian L. Müller (Munich)!
journals.plos.org/plosbiology/...

#ProudPI

New preprint out: www.biorxiv.org/content/10.1...
We followed up on our recent Nature Genetics paper mapping Shigella colonization in intestinal organoids with a deep dive into one of the screen hits: pcnB.

#Shigella #virulence #microsky

A scalable gut epithelial organoid model reveals the genome-wide colonization landscape of a human-adapted pathogen Nature Genetics - A genome-wide screen using human gut epithelial organoids combined with transposon-directed insertion sequencing identifies over 100 Shigella flexneri genes required for...

Our latest work combines human gut epithelial #organoid culture with experimental #Shigella #infections, transposon mutagenesis, and statistical modeling. This has led to the mapping of the comprehensive geneset that drives Shigella epithelial colonization.

Out @natgenet.nature.com:

rdcu.be/eqGgf

Do we really know the antibiotics that we already have?

Check out our story on 60y old drug nitroxoline and its new tricks!
@goetheuni.bsky.social @embl.org
www.nature.com/articles/s41...

+ great piece from the synchrotron @esrf.fr where we chased nx effects on metals
www.esrf.fr/home/news/ge...

Looking forward to speaking in this webinar series next Wednesday.

So happy to see the first paper on research from the lab out! Just a review for now, but it gives an idea of the avenues we are exploring.

PhD position in Chemistry, with a specialization in biochemistry The Department of Chemistry is opening a PhD position in Chemistry with a specialization in biochemistry. The thesis project will focus on drug resistance mechanisms in microbiome species, and is part

We have a PhD student position open in the context of the ENDAMR doctoral network. Deadline is 31/May. Apply here: umu.varbi.com/en/what:job/...

Looking forward to sharing with this community what our lab is doing