Domenic Narducci

(1/n) Thread @matteomazzocca.bsky.social @domenicnarducci.bsky.social Simon Grosse-Holz @jessematthias.bsky.social preprint

Q: how does chromatin move?

Using MINFLUX, SPT & SRLCI, we track chromatin dynamics across 7 orders of magnitude in time to provide answers www.biorxiv.org/content/10.1...

15.05.2025 12:37 — 👍 69    🔁 22    💬 2    📌 1

Very excited to have been a part of this great work with @matteomazzocca.bsky.social, Simon Grosse-Holz, @jessematthias.bsky.social, and @andersshansen.bsky.social. Please check it out now! www.biorxiv.org/content/10.1...

14.05.2025 18:03 — 👍 17    🔁 7    💬 0    📌 0

So I've gotten some secondary confirmation of this, sorta, though it's possible it's—this will shock you, surely—a bit chaotic in its implementation.

I'm also hearing that at least in some sub-institutes, phones and laptops are having location services turned on remotely where before they were off.

24.01.2025 22:40 — 👍 235    🔁 97    💬 5    📌 6

I am a biologist. This policy is based on disinformation and is also extremely harmful to many of our community members.

Here is your biological truth: there is no single and universal set of criteria that delineates or defines "distinct" sexes. We are a beautifully variable species!

20.01.2025 20:57 — 👍 121    🔁 47    💬 1    📌 1

A high-throughput platform for single-molecule tracking identifies drug interaction and cellular mechanisms Single-molecule tracking at scale, analyzing millions of cells and thousands of compounds per day, demonstrates that measuring protein motion provides mechanistic insights relevant to drug discovery.

A high-throughput platform for single-molecule tracking identifies drug interaction and cellular mechanisms.

19.01.2025 14:59 — 👍 12    🔁 3    💬 0    📌 1

Super-photostable organic dye for long-term live-cell single-protein imaging - Nature Methods Phoenix Fluor 555 (PF555) is a bright dye with an exceptional, order-of-magnitude longer photobleaching lifetime than conventional organic dyes that enables extended live-cell single-molecule imaging ...

www.nature.com/articles/s41...

18.01.2025 08:18 — 👍 28    🔁 4    💬 0    📌 1

Image showing a GUI with many numeric inputs on the left side and color coded intensity maps, illustrating a workflow, on the right side.

🔬peeps!
I am delighted to finally publish my code for controlling a spatial light modulator (SLM) to perform adaptive optics & to sculpt the vortex beams for STED & MINFLUX microscopy - complete w graphical user interface for easy use!
github.com/wiebkejahr/s...
Short user manual🧵

15.01.2025 15:31 — 👍 45    🔁 10    💬 4    📌 2

Brief thread on our opinion article "Q-rich activation domains: flexible ‘rulers’ for transcription start site selection?". Transcription people are surely aware of the important reports on the DNA sequence determinants of start site (TSS) selection in the human genome that came out this year. 1/15

22.12.2024 15:20 — 👍 15    🔁 9    💬 2    📌 0

ZNF143 is a transcriptional regulator of nuclear-encoded mitochondrial genes that acts independently of looping and CTCF ZNF143 has been implicated in gene activation and chromatin looping. Magnitov et al. show that ZNF143 has no general looping factor activity and explain why this function has been misattributed. Instead, ZNF143 regulates transcription of nuclear-encoded mitochondrial genes, ensuring energy homeostasis, proliferation, and differentiation potential of stem cells.

Please check out our new paper showing that the supposed looping factor ZNF143 has no role in chromatin looping: www.cell.com/molecular-ce...

And also check out the companion paper from @andersshansen.bsky.social: www.cell.com/molecular-ce...

20.12.2024 16:22 — 👍 63    🔁 30    💬 2    📌 2

Excited to share @domenicnarducci.bsky.social 's paper now out in Mol Cell published back-to-back with the paper from @dewitlab.bsky.social‬ - despite lots of prior work, we find that ZNF143 has no looping function
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...

20.12.2024 16:32 — 👍 65    🔁 20    💬 1    📌 0

www.biorxiv.org/content/10.1...
Our study published today on #bioRxiv describe the identification of a deaminase that converts 5mC to T, enabling direct sequencing of the human methylome and genome. This achievement was made possible through a collaborative effort across all departments at #NEB.

09.12.2024 14:12 — 👍 113    🔁 57    💬 5    📌 6

How did we get here, with a prominent anti-vaxxer nominated to lead Health and Human Services, with its $1.7T budget?

Much of it can be traced to this retracted 1998 paper, falsely linking the measles, mumps and rubella (MMR) vaccine to autism. 1/3

30.11.2024 02:05 — 👍 99    🔁 38    💬 7    📌 3