Pedro Brandão-Dias F Pinto

Being In an interdisciplinary field, it is easy to feel dumb because you are a "jack of all trades, master of none"

But that feel is greatly improved when you learn this sentence is often misquoted. The full version is:

"jack of all trades, master of none, but often better than a master of one"🎃

YouTube video by The Packard Foundation eDNA is Changing the Way We See the Ocean

Whales in a bottle of water? Yes… through eDNA. 🌊🐋

Nice feature on our research and the UW eDNA Collaborative by the Packard Foundation. @ednacollab.bsky.social

Learn more here:
www.packard.org/insights/gra...

www.youtube.com/watch?v=C0H8...
#eDNA #scicom #science #outreach

Estimating Organism Abundance Using Within‐Sample Haplotype Frequencies of eDNA Data Environmental DNA (eDNA) provides powerful insights into species presence and community composition but remains limited in its capacity to infer species abundance or population structure. Here, we sh...

🧬 My haplotype paper is out!

We show that deviations between within-sample and population-level haplotype frequencies can be used to estimate how many individuals contributed to an eDNA sample.

No tissue references needed, just metabarcoding data and some population genetics.

#eDNA #PopGen

Of course! Why did I expect it to be simple? 😂

Imagine the great quotes we would get if Darwin had to code!

I would see these guys on campus emerging out of the suspected N. valhalla twigs :)

Yes it is!

😂 right on! Luckily no Pikachu or Arrokuda hit me in the making of this video.

Babies may result 🥚🐣

The blue throat and the display of the Brandt's cormorants are pretty amazing.
#birding

Try working with 12S! It is a nightmare out here.

People insist on metabarcoding with 12S.

I cry "no, please, no, I cannot annotate these!"

But the cries are unheard. Family-level annotations follow;
Complaints come!

But what can I do? 1 SNP separates two paraphiletic genera.

And pseudogenes...

I have seen some convincing 3%'s

That would indeed save us some time. Great idea. I will start work now.

Please update me if you do it before me!

My high school math teacher was great but convinced I should not become a biologist because "my math intuition would be wasted!"

I just got a paper accepted with 10 equations plus more in the supplement 💀.

Pretty sure he would be happy but relieved they are printed and not in my handwriting 😄✍️📄

Integrating eDNA and acoustic-trawl data to provide small pelagic biomass estimates for fish stock assessment Abstract. Accurate abundance estimates of fisheries resources are essential for sustainable fisheries management. In response to the growing need for devel

New paper by Cristina Claver (@azti.bsky.social), published in @icesmarine.bsky.social JMS.

We combine #eDNA and acoustic-trawl data in a Bayesian joint model to estimate biomass of the European anchovy in the Bay of Biscay.

From the potential of eDNA to operational use

🔗 doi.org/10.1093/ices...

Congrats!! Cool paper. Added to my to-read list :)

Laboratory Study on Fate and Transport of Water-Borne eDNA: A Tale of Tempered Tails and Parafluvial Processes Environmental DNA (eDNA) has emerged as a powerful marker for assessing species presence in flowing waters; however, estimating the spatial and temporal origins of these signals remains a challenge. T...

Big milestone today: My first PhD paper is officially out! We took a deep dive into eDNA transport dynamics in flowing waters. Check out the full study in ES&T.

pubs.acs.org/doi/full/10....

a white seagull with a yellow beak is standing on a green roof with failarmy written on the bottom ALT: a white seagull with a yellow beak is standing on a green roof with failarmy written on the bottom

Me when I see a juvenile gull in the Pacific Northwest on an impatient day:

NOPE
~confidently logs Gull sp. on eBird~

Me on a patient day:
stares at juvenile gull for 10 minutes

“Yeah… that’s definitely a Gull sp.”
✔️ eBird submitted
#birding

Hahah it does look comical! But I mean... we don't wanna risk it moving mid sequencing!. These are the conditions most of the time.

(It was relatively tame at the time I took this video)

We will know soon!

We are on!

Air 🌬️
Sea spray 💦
Surface 🌊
3000 m deep ⚓

~1 million DNA molecules/hour
sequenced live in the middle of the Pacific Ocean!

Shout out to Aden and Rhonda,l who can extract DNA, PCR, and library prep without getting seasick ⛵️

From sample to data in less than 48 hrs.

#eDNA #nanopore #UW

Sea cowboys are at it again! A cast every 4 hours for our Shenanigans.

#eDNA #CRESST Cruise

Gotta stay organized! #eDNA

Congrats!!!

Sunrise CTD cast!! This one went right inside the Toto caldera, which is an underwater volcano 🌋!

We found the volcano did have a plume (meaning it is activelly erupting) - I got to see scientists cheering to a live feed of data when the plume appeared. NASA landing style 🚀👩‍🚀 #Cruise #Volcano

Ahaha maybe after the final extraction as a celebration!

We call this trick "we forgot to pack a heatblock and now we have to improvise with a hotplate and sand".

It is walnut shell sand. Smells nice.

Our first #CRESST CTD cast is out!

A CTD is a rosette sampler full of sensors and water bottles. We can monitor sensors live and trigger the closure of each bottle from aboard as it comes back up through the water column (deep to shalow).

We don't have much time, so this cast was only to 200m.

The Cruise is delayed one more day because the chemicals did not arrive.
What do a group of bored scientists do?
Come up with a new experiment, of course! #eDNA #rain

After a 7 day delay, we are finally on board of the RV Thomas G. Thompson! Getting ready for 14 days of #eDNA data collection and lab work going from #Philippines towards #Guam

It is a data deficient area, so I will also be making the first ever pelagic bird surveys of this region.🐦‍⬛🐦 #unqualified