Furukawa Lab @CSHL

Our paper on conductance control and neurosteroid binding in NMDAR has been officially published. I have attached a movie that describes a sub-open and a full-open channel. Also, neurosteroid binding patterns are surprisingly quite diverse www.nature.com/articles/s41...

Hiro Furukawa @lab-furukawa.bsky.social provides commentary on new @jgp.org study by Lotti et al. (rupress.org/jgp/article/...) which reports a new #NMDAR subtype-selective allosteric inhibitor with improved potency and elucidate its mechanism of inhibition. rupress.org/jgp/article/...

Structural insights into mechanisms of zinc scavenging by the Candida albicans zincophore Pra1 - Nature Communications During Candida albicans infection, the host restricts zinc to limit fungal growth. Here, the authors present cryo-EM structures of the C. albicans zincophore and genetically show that intact zinc-bind...

www.nature.com/articles/s41... The first paper from the lab led by the HF lab alumna Johanna Syrjanen. www.syrjanenlab.com A big congratulations!

Our latest work : Statins are used to lower plasma cholesterol but often come with muscle-related side effects. Using cryo-EM, we show how multiple statin molecules cooperate to bind RyR1, a calcium release channel mainly found in skeletal muscle.
nature.com/articles/s41...

Assistant Professor in Biology and Neuroscience Program - Waltham, Massachusetts (US) job with Brandeis University | 12848464 The Department of Biology and the Program in Neuroscience at Brandeis University invite applications for a tenure-track Assistant Professor positio...

Re-upping this - Neuropeeps! We have a TT Job in my wonderful department at Brandeis! First consideration to applications received by Dec 15 (but why wait?)

www.nature.com/naturecareer...

Now in the journal issue! Molecular mechanism of activation of kainate receptor GluK2 in complex with auxiliary subunit Neto2.
www.nature.com/articles/s41...

--Postdoctoral Fellowship in Structural Neurophysiology at CSHL— Post a job in 3min, or find thousands of job offers like this one at jobRxiv!

We have postdoctoral positions available for patch-clampers and structural biologists #cryo-EM #electrophysiology #cancer-neuroimmunology #neuropharmacology jobrxiv.org/job/cold-spr...

Calcium and magnesium in the pore of NMDA receptors!

Lipids play an unexpected role. The hydration shells of Ca2+ and Mg2+ could be the difference between permeation and block. So much physiology in the energetics of H2O.

Very stimulating work! Congrats @lab-furukawa.bsky.social

#iglurs

Thank you, Andrew!!

Cofilin Inhibition Ameliorates PIEZO2 and AMPA Dysfunction in a Mouse Model of Angelman Syndrome Angelman syndrome (AS) is a neurogenetic disorder characterized by motor coordination and cognitive deficits. In AS, hippocampal neurons show reduced filamentous (F-)actin, a decrease we also reported...

Our new Journal of Neuroscience paper "Cofilin Inhibition Ameliorates PIEZO2 and AMPA Dysfunction in a Mouse Model of Angelman Syndrome (AS)” is out: www.jneurosci.org/content/45/4... 1/6

Magnesium site in TRPV6 is formed by two negatively charged residues that are close together in the closed state and separated in the open. Magnesium binding keeps them together and stabilizes the closed state. Amazing collaboration with Chubanov and Efremov groups. www.nature.com/articles/s41...

Cornell BME at Weill Hall in Ithaca

My department, Cornell BME, is hiring!

This tenure-track faculty position is a great opportunity to join a vibrant community that values research, teaching, and entrepreneurship.

📝 academicjobsonline.org/ajo/jobs/30816

A doorstop for the brain’s electrical gates | Cold Spring Harbor Laboratory As information zings from cell to cell inside the brain, bursts of electricity spur its transmission. At Cold Spring Harbor Laboratory (CSHL), scientists have turned their attention to the tiny pores ...

A new study from the Furukawa lab (@lab-furukawa.bsky.social) identifies the neurological ‘doorstops’ that keep the brain’s NMDA receptors fully or partially open. The findings could have implications for treating neurodegenerative conditions like #Alzheimers disease. www.cshl.edu/a-doorstop-f...

Mechanism of conductance control and neurosteroid binding in NMDA receptors - Nature Ion-channel conductance levels are controlled by the bending patterns of pore-forming transmembrane helices in the heterotetrameric neuronal channel GluN1a-2B N-methyl-D-aspartate receptor.

How are NMDA receptor channel conductance states regulated? We uncover a mechanism for tuning ion flow and reveal striking diversity in neurosteroid binding. Our structures captured two distinct open-channel states. A fantastic collaboration with the Traynelis lab.
www.nature.com/articles/s41...

Apply to the new CSHL grad program in BioAI/neuroAI!

(Masters degree or equivalent required in a quantitative area such as computer science, physics, mathematics, or engineering)

www.cshl.edu/phd-program/...

Assistant Professor in Structural Biology and Biochemistry, Department of Molecular Biology & Biochemistry University of California, Irvine is hiring. Apply now!

Our home department of Molecular Biology & Biochemistry at UC Irvine is looking to hire a new tenure-track assistant professor in the broad area of structural biology. Come be our colleague! recruit.ap.uci.edu/JPF09887

Please apply and/or share this post.

UGA is seeking a tenure-track Assistant Professor to develop an innovative research program focused on disease mechanisms. The ideal candidate will use cryo-EM and other structural techniques, with potential applications to future therapies.
Apply here: www.ugajobsearch.com/postings/456...

The Department of Biochemistry and Molecular Biology at UTHealth Houston has an open search for a tenure-track faculty position. If you are ready (or know someone that is) to start looking for positions, this will be a great opportunity to join the Texas Medical Center. See flyer below. 1/2

PhD project alert: the excellent MIBTP programme is open for applications. I am offering an exciting project on endogenous membrane protein complexes, jointly supervised with @alangoddard.bsky.social. Please spread the word!
warwick.ac.uk/fac/cross_fa...

The Rutgers Brain Health Institute @rutgersbhi.bsky.social is looking for an accomplished scientist to serve as the Director of the new Rutgers Center on Research in Pain and Pain Therapeutics. Feel free re-post and/or reach out to me if you have any questions
jobs.rutgers.edu/postings/225...

--Postdoctoral Fellowship in Structural Neurophysiology at CSHL— Post a job in 3min, or find thousands of job offers like this one at jobRxiv!

We have postdoctoral positions available for patch-clampers and structural biologists #cryo-EM #electrophysiology #cancer-neuroimmunology #neuropharmacology jobrxiv.org/job/cold-spr...

Inhibition of GluN2B-containing N-methyl-d-aspartate receptors by radiprodil Banke et al. identified the structural determinants of radiprodil binding and the mechanism by which it inhibits GluN2B-NMDARs. They found that radiprodil

academic.oup.com/brain/advanc... with Hongjie Yuan and Steve Traynelis. We went back to x-ray crystallography to capture radiprodil, a classic psychiatric compound.

‘Mini analysis’ misrepresents changes in synaptic properties due to incomplete event detection Abstract figure legend Summary of the study where simulated recordings (left) were used to characterise the effect of incomplete detection on mini (mPSC) analysis. Recording noise levels (red) determ...

Our paper on 'mini analysis' is now published in @jphysiol.bsky.social
If you are recording mPSCs or sPSCs, I hope this helps with analysis. Interpretation of these datasets is not as easy as it seems..!
Happy to discuss if you are interested
physoc.onlinelibrary.wiley.com/doi/10.1113/...

Congrats and welcome to the east coast!!

Congrats! The paper looks nice!

Cryo-EM snapshots of NMDA receptor activation illuminate sequential rearrangements Opening of the NMDA receptor involves bending of the helices lining the ion channel gate.

Cryo-EM snapshots of NMDA receptor activation illuminate sequential rearrangements | Science Advances www.science.org/doi/10.1126/...

🚀 Check out our new preprint on extending the scope of single-particle cryo-EM with 2D template matching!
Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching is now available on bioRxiv!
www.biorxiv.org/content/10.1...

After years in making, I am glad to be able to share our attempt on establishing a framework for combined functional and computational assessment of variant pathogenicity in the sodium leak channel NALCN:
www.biorxiv.org/content/10.1...

🚨🧠🔬 A major breakthrough in molecular neuroscience:

I am excited to share a new story from our lab, published in accelerated format today by @nature.com:

"Delta-type glutamate receptors are ligand-gated ion channels"

Read more here (free article link): rdcu.be/eGIKz

We have two postdoctoral fellow positions—one for a structural biologist and another for an electrophysiologist. We have new, exciting projects related to neurobiology, neuro-cancer-immunology, and neuropharmacology. #electrophysiology #cryo-EM #neuro-xyz