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Greg Madej

@greg-madej.bsky.social

University of Regensburg | #cryoEM | #membrane #proteins

79 Followers  |  157 Following  |  28 Posts  |  Joined: 11.02.2025  |  2.2144

Latest posts by greg-madej.bsky.social on Bluesky

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The @ziegler-lab-ur.bsky.social delegation survived the Munich Half Marathon! This Trial of Spirit demanded the Force be strong with us. And the Force was extraordinarily strong with @katjaurbansky.bsky.social Her exceptional pace has earned her a promotion to the Council of cryoKnights - so cool!

14.10.2025 04:19 β€” πŸ‘ 4    πŸ” 2    πŸ’¬ 0    πŸ“Œ 0
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Enjoy the picture to see moments from the visit β€” from the Robin Hood statue to the lab and evening strolls through Nottingham! 🌿

@uniregensburg.bsky.social

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07.10.2025 18:31 β€” πŸ‘ 1    πŸ” 1    πŸ’¬ 0    πŸ“Œ 0
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The visit highlighted the spirit of our project: connecting scientists across borders and disciplines to advance cutting-edge research together to uncover the mechanisms plants use to perceive water stress.

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07.10.2025 18:31 β€” πŸ‘ 1    πŸ” 1    πŸ’¬ 1    πŸ“Œ 0
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During this visit, Christine Ziegler @ziegler-lab-ur.bsky.social joined Malcolm Bennett in their lab at the University of Nottingham, while Eilon Shani @eilonshani.bsky.social also happened to be visiting the same research center β€” a perfect coincidence for a productive and friendly reunion!

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07.10.2025 18:31 β€” πŸ‘ 3    πŸ” 2    πŸ’¬ 1    πŸ“Œ 0
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I've just commissioned a cryoEM new coffee mug in new design. To be fair, the mug is arguably the more critical piece of equipment for data collection. You try imaging a membrane proteins at 5 AM without caffeine. β˜•πŸ”¬#cryoEM

09.10.2025 03:40 β€” πŸ‘ 3    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
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MC 2025: We came, we saw, we wonπŸ† πŸ†! Amazing team on a fantastic time at the Microscopy Conference in Karlsruhe.
Get this: two of our posters won Poster Prizes! We're pretty sure it was our perfect blend of science and questionable doodles in the margins. @ziegler-lab-ur.bsky.social

04.09.2025 09:44 β€” πŸ‘ 4    πŸ” 3    πŸ’¬ 0    πŸ“Œ 0
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Ultrafast Almost Gold!
The Regensburg Center for Ultrafast Nanoscopy bio-section took second in the relay! Physics' reaction rates are attoseconds, but ours, Slower. Probably why "The Quantum Leapers" edged us out. Great fun and pretzels! @ziegler-lab-ur.bsky.social @katjaurbansky.bsky.social

17.07.2025 14:44 β€” πŸ‘ 4    πŸ” 1    πŸ’¬ 0    πŸ“Œ 1

So exciting … The publication where I had the privilege to contribute has been selected as an Editor's Choice. This recognition truly reflects everyone's hard work and dedication.

26.06.2025 15:51 β€” πŸ‘ 3    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
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Recently, our early-career researchers agreed on the value of organizing regular sessions for PhDs and PostDocs to explore key research topics in greater depth.

These sessions offer a space to understand the methods & techniques used across the 4 #Hydrosensing teams.

#science

12.06.2025 10:53 β€” πŸ‘ 6    πŸ” 1    πŸ’¬ 1    πŸ“Œ 0
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In the very 1st session this Tuesday, @greg-madej.bsky.social, from @uniregensburg.bsky.social @ziegler-lab-ur.bsky.social, presented how their expertise is applied within the project; specially the use of Cryo-EM in the study of membrane-embedded proteins.

🌐 www.uni-regensburg.de/biologie-vor...

12.06.2025 10:53 β€” πŸ‘ 4    πŸ” 2    πŸ’¬ 1    πŸ“Œ 0
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Yesterday, through the tireless work of training runs, the support of my team, and the simple act of putting one foot in front of the other, we reached the finish line. The justice of completing what we set out to do, after the planning and the effort, has been served.
@katjaurbansky.bsky.social

02.06.2025 06:28 β€” πŸ‘ 1    πŸ” 1    πŸ’¬ 0    πŸ“Œ 0
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A conserved nuclease facilitates environmental DNA uptake Abstract. Bacteria acquire new traits through the uptake of genetic material from the environment, a process requiring DNA processing. However, the molecul

academic.oup.com/nar/article/...
Proud to have been a small part of the team behind the latest publication. It was a privilege to collaborate with such a talented group of researchers on this work. A massive congratulations to all the authors for this contribution to the field!
#RNA #cryoEM

27.05.2025 15:02 β€” πŸ‘ 3    πŸ” 2    πŸ’¬ 0    πŸ“Œ 0
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FEBS Press Mutations in polycystin-1 (PC1) or polycystin-2 (PC2) cause autosomal-dominant polycystic kidney disease (ADPKD). We generated a novel gain-of-function PC2/PC1 heteromeric ion channel by mutating por...

Here is an update on our recent activity.. febs.onlinelibrary.wiley.com/doi/10.1002/...

12.05.2025 11:42 β€” πŸ‘ 2    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
Malcolm Bennet in the greenhouse with the filming crew

Malcolm Bennet in the greenhouse with the filming crew

Poonam Mehra while filming

Poonam Mehra while filming

Malcolm Bennet in the greenhouse while filming

Malcolm Bennet in the greenhouse while filming

In @uniofnottingham.bsky.social ERC Hydrosensing members spent a few intense (and wardrobe-consistent πŸ˜…) days filming about research on root systems and how plants adapt to drought.

More footage for the documentary is being filmed at locations around the world in the coming year.

22.04.2025 10:48 β€” πŸ‘ 11    πŸ” 3    πŸ’¬ 1    πŸ“Œ 0
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Retinal to Retinal Energy Transfer in a Bistable Microbial Rhodopsin Dimer Neorhodopsin (NeoR) is a newly discovered fungal bistable rhodopsin that reversibly photoswitches between UV- and near-IR absorbing states denoted NeoR367 and NeoR690, respectively. NeoR367 represents...

We had the pleasure to contribute to this publication... It was a pleasure to be part of this effort. Congratulations to all authors! #cryoEM
@cmziegler.bsky.social
@greg-madej.bsky.social
pubs.acs.org/doi/full/10....

22.04.2025 09:17 β€” πŸ‘ 5    πŸ” 1    πŸ’¬ 0    πŸ“Œ 0

These were great days.. thank you all!
@hamannlab.bsky.social @eilonshani.bsky.social @sara-morghen.bsky.social @francesco-saffioti.bsky.social @katjaurbansky.bsky.social @wiebkehaeger.bsky.social @michaelaticha.bsky.social @hydrosensing.bsky.social @hydrophotonics.bsky.social

17.04.2025 12:22 β€” πŸ‘ 5    πŸ” 2    πŸ’¬ 0    πŸ“Œ 0

First images coming soon 🀩

17.04.2025 05:53 β€” πŸ‘ 13    πŸ” 2    πŸ’¬ 2    πŸ“Œ 0

Congratulations...!

17.04.2025 10:13 β€” πŸ‘ 0    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
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#HydroSensing Synergy Group - your fascinating talks last week completely watered the seeds of curiosity in my brain πŸ˜‰. Prepare to see the photographic evidence of my descent into macroscale hydrosensing madness! I'm pretty sure my leafy friends 🌱 are judging my overly enthusiastic note-taking.

15.04.2025 15:45 β€” πŸ‘ 3    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
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We are fascinated by how our leafy friends 🌱 feel the subtle shifts in soil moisture πŸ’¦ Last week we hydrated your minds and cultivated connections at our #erc meeting focusing on the captivating world of #Plant #HydroSensing 🧩@hydrosensing.bsky.social @hydrophotonics.bsky.social

14.04.2025 18:20 β€” πŸ‘ 4    πŸ” 5    πŸ’¬ 0    πŸ“Œ 0
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Exciting ERC synergy project meeting in Regensburg 😊@hydrosensing.bsky.social @ntnudiscovery.bsky.social @uniofnottingham.bsky.social @uniregensburg.bsky.social

11.04.2025 08:30 β€” πŸ‘ 8    πŸ” 3    πŸ’¬ 0    πŸ“Œ 0
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After our annual #HydroSensing meeting in Regensburg, we went for a hike.. of course with a subsequent data collection and data processing demonstration 😁 #Plant #cryoEM

13.04.2025 14:29 β€” πŸ‘ 5    πŸ” 2    πŸ’¬ 0    πŸ“Œ 0

It needs some practice, but the whole process: cooling the box, clipping grids, loading the autoloader takes about 20-30 min for 4 grids. Originally the process you see in the movie is 2 min - I have speeded up the frame rate 😁

03.03.2025 20:10 β€” πŸ‘ 0    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0

light-sensitive compounds that do not absorb in red light.. πŸ˜‰

03.03.2025 19:31 β€” πŸ‘ 1    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
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Yes, we do wear a mask and the room is somewhat humidity controlled.. at hot summer days not so much πŸ˜‚ With a little practice, clipping is also possible at limited visibility or if you prefer red light πŸ˜‰ When the rel. humidity climbs >45% things start getting challenging πŸ˜₯

03.03.2025 17:56 β€” πŸ‘ 1    πŸ” 0    πŸ’¬ 1    πŸ“Œ 0

It’s really easy.. we let it students do it in a practical class πŸ˜‰ You will enjoy it πŸ‘

03.03.2025 17:38 β€” πŸ‘ 0    πŸ” 0    πŸ’¬ 1    πŸ“Œ 0
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the cryo-clipping-box for JEOL cryoARM... easy! #cryoEM

03.03.2025 15:55 β€” πŸ‘ 4    πŸ” 1    πŸ’¬ 2    πŸ“Œ 0
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We've been using SerialEM 4.2.0 beta on our JEOL cryoARM-200 for a while now. The multi-record setup and coma vs. IS calibration routines are particularly helpful – they're user-friendly and consistently reliable. #cryoEM #SerialEM

01.03.2025 12:53 β€” πŸ‘ 5    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
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Just another Friday evening cryoEM screening.. with pizza 😁 #cryoEM

28.02.2025 16:49 β€” πŸ‘ 6    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0

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