Grateful to my colleagues at @vicidominilab.bsky.social @iitalk.bsky.social for their support in making this research possible, and to @erc.europa.eu and #MSCA for their support! 6/6
04.07.2025 07:37 — 👍 3 🔁 1 💬 0 📌 0
ISM-FLUX offers more than just a simple experimental setup! Discover how the doughnut-shaped illumination provides a homogeneous localization uncertainty over a large FOV and learn about the self-calibrating procedure of ISM-FLUX. Check out our paper for all the details. 5/6
04.07.2025 07:35 — 👍 2 🔁 1 💬 1 📌 0
We tested ISM-FLUX on DNA-origami nanorulers, and achieved a median localization uncertainty below 5 nm for single molecules. This precision effortlessly resolved 40 nm and 20 nm nanorulers. 4/6
04.07.2025 07:35 — 👍 2 🔁 0 💬 1 📌 0
Similar to #MINFLUX, our localization uncertainty scales with L/sqrt(N), with L the TCP diameter and N the number of photons. However, while MINFLUX requires the emitter to be within the scanned area, ISM-FLUX has a much larger localization range, making it more robust. 3/6
04.07.2025 07:34 — 👍 2 🔁 1 💬 1 📌 0
ISM-FLUX makes use of a #SPAD array detector. This boosts localization range and offers flexibility in the scanned target coordinate pattern (TCP). We chose a circular pattern, enabled by galvanometric scan mirrors found in most LSMs. 2/6
04.07.2025 07:31 — 👍 3 🔁 1 💬 1 📌 0
Our last paper on s²ISM (super-resolution sectioning #ImageScanningMicroscopy) just got published on Nature Photonics @natphoton.nature.com! Discover how structured detection in laser scanning microscopes enhances both resolution and optical sectioning:
www.nature.com/articles/s41...
🧵1/7
06.06.2025 05:40 — 👍 14 🔁 5 💬 2 📌 0
Physicist interested in classical and quantum optics.
All-around climber and amateur alpinist.
https://alessandro-zunino.github.io/
Cancer cell biologist | microscopist. MS-Biotechnology| PhD-Physics 🔬Postdoc@BakhoumLab,MSK. Mitochondrial ROS | micronuclei | nanoscopy. On the academic job market| viewsAreMyOwn
Studying genome organization with quantitative super-resolution microscopy | Postdoc at Harvard/Wyss | PhD at MPIB and LMU
https://www.researchgate.net/profile/Johannes-Stein-4
Built to unlock the power of single-molecule fluorescence spectroscopy
CEO at Exciting Instruments
Democratising Single Molecule Detection
PI - @craggs-lab
“A collision of Science and Religion”
William J. Link Chair and Professor
Department of Biomedical Engineering
University of California Irvine
Professor, Physiology Department, Perelman School of Medicine, University of Pennsylvania. https://www.lakadamyali-lab.com
Super resolution microscopy, single molecule biophysics, microtubules, organelles, nucleus and chromatin
The Ries group develops superresolution microscopy to study the structure and dynamics of proteins, ultimately in the living cell.
@maxperutzlabs.bsky.social @univie.ac.at
#SMLM, #MINFLUX, #endocytosis, #AI4microscopy
We work on the development of “smart” fluorescent probes, improving fluorescence imaging, biosensors and image analysis software. Account managed by lab members. More on https://peterdedecker.be/.
Assistant Professor at University of Warwick
Postdoc in Fraser Lab (USC)
Postdoc Fritzsche Lab (Oxford)
PhD in Eggeling Lab (Oxford)
Into all things Biophysics, Fluorescence, and Membranes
https://faldalf.github.io
Professor of Physics (https://www.joerg-enderlein.de), Editor-in-Chief of Biophysical Reports (https://www.cell.com/biophysreports/home), Science & Arts, 🏳️🌈 | biophysics | single-molecule spectroscopy | super-resolution microscopy | nano-optics & plasmonics
💻 🧬 3D genome & RNA lovers Group Leader at Center for Human Technologies, Italian Institute of Technology | CDA @ArmeniseHarvard
Molecular Microscopy and Spectroscopy Lab (https://vicidominilab.github.io) at the @iitalk.bsky.social.
"A #SPADarray detector in any laser-scanning microscope"
Welcome to the official account of SPIE, the international society for optics and #photonics. Over the past five years, we have invested over $25M in the international optics community! 💡
We are Europe's gateway to biological & biomedical imaging expertise. Visit our website: www.eurobioimaging.eu
Profile image by Olivia Harris, Cambridge University.
#RSE for @gerbi-gmb.de, TA1 lead for @nfdi4bioimage.bsky.social, #OSS Maintainer for @openmicroscopy.org and @zarr.dev
VIB BioImaging Core in Leuven, microscopes, image analysis, neurons, tomography, sometimes food, weird things, life, opinions are my own
PhD in BioPhysics.
Scientist who worked at the Genome Institute of Singapore. Worked on 3D lightsheet and confocal microscopy for 3D spatial transcriptomics via multiplexed error robust fluorescence in situ hybridisation (MERFISH).
Biomedical Computer Vision & Language Models, scads.ai / Leipzig University, also NFDI4BIOIMAGE, NEUBIAS/GloBIAS, GPUs, AI, ML 🔬🖥️🚀 views:mine
