Sunil Kumar Pradhan

New method paper:
Bennie Lemmens et al @scilifelab.se describe 3D-SPARK, a super-resolution microscopy method that maps changes in DNA replication nanostructures in response to genetic perturbations or drugs, linking spatial cell biology with DNA synthesis dynamics
www.embopress.org/doi/full/10....

07.10.2025 13:04 — 👍 15    🔁 8    💬 0    📌 0

We finally reveal a strand-specific role of MajSat RNA by using forward / reverse strand-specific locked nucleic acid DNA gapmers. We found the forward MajSat RNA to be regulating the replication timing of the PCH, suggesting ncRNA as regulators of replication timing.

10.09.2025 08:50 — 👍 1    🔁 0    💬 0    📌 0

Loss of H3K36me3 at pericentromeric heterochromatin disrupts transcription:

↑ RNA Pol II Ser5 (initiation)

↓ RNA Pol II Ser2 (elongation)

↓ Major satellite RNA levels

Together, these changes point to transcriptional stalling and loss of MajSat RNA.

10.09.2025 08:50 — 👍 0    🔁 0    💬 1    📌 0

When we depleted H3K36me3 specifically from pericentromeric heterochromatin, replication timing was disrupted.
⚡ Instead of a neatly ordered sequence, PCH began replicating together with other heterochromatic regions - a loss of temporal control linked to genome instability.

10.09.2025 08:50 — 👍 0    🔁 0    💬 1    📌 0

To probe the role of H3K36me3 at PCH, we developed a new approach:
✨ Nanobody-mediated targeting of oncohistone H3.1 K36M directly to pericentromeric heterochromatin.
This locally reduced H3K36me3 without altering other histone modifications, and elsewhere in the chromatin.

10.09.2025 08:50 — 👍 3    🔁 1    💬 1    📌 0

Different perspectives on epigenetic dynamics and memory, especially before genome replication:
We show H3K36me3 is dynamically enriched at pericentromeric heterochromatin (PCH) before its replication and erased after, tightly linking epigenetics and replication timing. doi.org/10.1038/s443...

10.09.2025 08:50 — 👍 6    🔁 1    💬 1    📌 1

Exposure of cells to near-infrared irradiation relaxes chromatin compaction and facilitates recognition of cyclo-butane pyrimidine dimers - Scientific Reports Scientific Reports - Exposure of cells to near-infrared irradiation relaxes chromatin compaction and facilitates recognition of cyclo-butane pyrimidine dimers

🧬⚡Your sunscreen is only half the story
We show that near-infrared light makes UV damage WORSE. nIR loosens DNA packaging, creating a repair traffic jam that increases breaks. We are ignoring the invisible majority of solar radiation that hacks DNA repair.
doi.org/10.1038/s415...
@tuda.bsky.social

07.07.2025 13:15 — 👍 0    🔁 1    💬 0    📌 0

Warmest congratulations to Dr. Sunil K. Pradhan (@sunil-k-pradhan.bsky.social) on successfully defending his PhD thesis on genome replication and epigenetic regulation in mammalian cells! Wishing you continued success as you embark on your next chapter. 🎓"

14.02.2025 11:54 — 👍 3    🔁 1    💬 0    📌 0

Could I be added? Thanks!

10.12.2024 11:32 — 👍 0    🔁 0    💬 1    📌 0