László Tora

Exquisite work. Well done to the whole team.

🚨 New preprint out! Do you think Single Molecule Footprinting and Fiber-seq are super cool but aren't sure how to unlock their full potential? HiddenFoot can help you: www.biorxiv.org/content/10.1...

Friday we celebrated my 40 years of scientific career in France, with present and past lab members and many friends. It was a fantastic day full of emotions, memories and great science. Thanks for making the day so memorable !

The importance of moving away from bulk! Occupancy of Pol II at promoters is dramatically different between fly and mouse cells! When looking single molecule! Proud of the team! @kasitc.bsky.social @molinalab.bsky.social doi.org/10.1038/s443...

Two more weeks to send us your abstract! >20 abstract selected talks. Priority to junior scientists and cool biology on gene regulation (DNA/RNA/protein). Come to meet the world leaders in single molecule genomics and single molecule imaging! Help shaping the future of the field in the workshops!

👍

PMID: 40106549, DOI: 10.1126/sciadv.adr1492

Read our collaborative paper
@longchrom.bsky.social and @molinalab.bsky.social
showing that disruption of NuA4 and ATAC coactivator complexes cause gene-specific transcript buffering, suggesting that cells dynamically adjust RNA export/degradation to sustain cellular homeostasis.

Safeguarding the future of biomedical science in the United States NIH’s abrupt decision to cap indirect cost reimbursement at 15% threatens the critical infrastructure supporting groundbreaking biomedical research in the United States. This policy jeopardizes Americ...

www.cell.com/cell/fulltex...

Friends! The science that we love is under threat. World-wide, there're forces seeking to make it corporate-like, focused on deliverables, or even the enemy of society. Take a stand & celebrate science & music! #theconferencetoendallconferences - Registration starts NOW!
woodstock.img.cas.cz

Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma The midnolin-proteasome pathway degrades many nuclear proteins without ubiquitination, but how it operates mechanistically remains unclear. Here, we present structures of the midnolin-proteasome compl...

Thrilled to share our collaborative work on the structural basis and myeloma disease link of the midnolin-proteasome pathway with Chris Nardone from the Elledge lab, Jingjing Gao from the Shao lab @sshaolab.bsky.social and all the co-authors who put in the hard work! www.biorxiv.org/content/10.1...

‼️ This Wednesday! Don’t forget to register as we host three amazing ECR scientists!
@manucazottes.bsky.social @robertadolling1.bsky.social
@leandrosboukas.bsky.social

⚡️Our recurring registration link:

us06web.zoom.us/webinar/regi...

Intrinsically disordered regions as facilitators of the transcription factor target search - Nature Reviews Genetics Intrinsically disordered domains (IDRs) are increasingly appreciated as important components of protein function. This Perspective discusses the emerging evidence for IDRs from transcription factors a...

Interesting piece on the role of intrinsically disordered regions in #transcriptionfactor specificity, target search etc. ➡️ www.nature.com/articles/s41... #generegulation

Search gets faster, binding longer, bound fraction of cohesin and CTCF goes up, especially at and after ZGA

Increasingly efficient chromatin binding of cohesin and CTCF supports chromatin architecture formation during zebrafish embryogenesis - Nature Communications Using single-molecule tracking of cohesin and CTCF during zebrafish embryogenesis, the molecular kinetics underlying chromatin architecture formation was revealed and gradual emergence of chromatin st...

Single molecules, in living zebrafish embryos, during hour-long period of development: Jonas' work on binding kinetics of cohesin and CTCF around ZGA now online. Great collab with labs of @lucagiorgetti.bsky.social @akispapantonis.bsky.social
doi.org/10.1038/s414...

Gene regulation: one molecule at a time

Program is out for our first EMBL #singlemolecule gene regulation meeting! Check out the dedicated panel sessions on single molecule genomics, single molecule imaging and theory where we would like to brainstorm the future of the field:
Registration is open! 👇
www.embl.org/about/info/c...

The latest publication from our lab is online in @narjournal.bsky.social . Congratulations to @marcelwerner.bsky.social and Manuel Trauner for developing a novel system in mammalian cells to study Transcription-Replication conflicts and pointing DOT1L as a transcription mediator at TRC sites!

CRL3ARMC5 ubiquitin ligase and Integrator phosphatase form parallel mechanisms to control early stages of RNA Pol II transcription Cacioppo, Gillis, Shlamovitz, Zeller, et al. show that CRL3ARMC5 ubiquitylates RNA Pol II at early stages of transcription as part of a major homeostatic RNA Pol II turnover mechanism. CRL3ARMC5 and the Integrator phosphatase act as complementary pathways, monitoring the quantity and quality of RNA Pol II complexes before they are licensed into elongation.

The first paper from our lab is online! A wonderful collaboration with @scottbscience.bsky.social lab. We discover that Pol II is extensively regulated by Ubiquitin at the beginning of the transcription cycle!
www.cell.com/molecular-ce...

Today is #WorldCancerDay. Behind every diagnosis is a person, family, and friends who feel the devastating effects of cancer.

Our work has never been more vital.

With pioneering cancer research and groundbreaking discoveries, we can continue bringing hope to future generations.

Friday Lectures – Giacomo Cavalli

Join us on Friday, 7th February at 3pm for a talk by Giacomo Cavalli, expert in Polycomb regulation and 3D genome architecture.
He’ll explore how these mechanisms affect epigenetic inheritance and cancer development.
Don’t miss it!
#Epigenetics #Genomics #Cancer

My group @biovitenskap.bsky.social is #hiring a Post-doc in bioinformatics and biostatistics to work on #epigenetic memory in stem cells and during cell reprogramming. The position is co-supervised by @amathelier.bsky.social from @nordicembl.bsky.social

An atlas of transcription initiation reveals regulatory principles of gene and transposable element expression in early mammalian development Mapping transcription start sites across five mammalian species before, during, and after embryonic genome activation unveils widespread transposable element-driven transcription and co-option of evol...

Tour de force! @metorrespadilla.bsky.social & team develop Smart-seq+5′ to map TSS in single embryos with unprecedented res revealing plethora of TE-driven transcripts in 5 mammal species. Some surprisingly old & conserved. Treasure trove of new biology for yrs to come!
www.cell.com/cell/fulltex...

In a great collaboration with @hummerlab.bsky.social and the Kräusslich lab: HIV capsid doesn't break at the NPC; instead, it cracks open the NPC itself! Details in Cell: authors.elsevier.com/sd/article/S... @mpibp.bsky.social @uniheidelberg.bsky.social A thread below:

In this study, Davidson et al. describe an alternative but conserved mechanism for RNA polymerase II transcription termination that occurs directly at T-tracts and independent of polyadenylation site-directed termination. #openaccess

Learn more here:
➡️ tinyurl.com/gd351978

Brief thread on our opinion article "Q-rich activation domains: flexible ‘rulers’ for transcription start site selection?". Transcription people are surely aware of the important reports on the DNA sequence determinants of start site (TSS) selection in the human genome that came out this year. 1/15

EXTRA-seq: a genome-integrated extended massively parallel reporter assay to quantify enhancer-promoter communication Precise control of gene expression is essential for cellular function, but the mechanisms by which enhancers communicate with promoters to coordinate this process are not fully understood. While seque...

Finally out! We present EXTRA-seq, a new EXTended Reporter Assay to quantify endogenous enhancer-promoter communication at kb scale!
www.biorxiv.org/content/10.1...
A 🧵about what it can do:
#SynBio #DeepLearning #GeneRegulation

ATAC and SAGA histone acetyltransferase modules facilitate transcription factor binding to nucleosomes independent of their acetylation activity Abstract. Transcription initiation involves the coordination of multiple events, starting with activators binding specific DNA target sequences, which recr

Thanks Manuel.This should be the right link
academic.oup.com/nar/advance-...

ATAC and SAGA histone acetyltransferase modules facilitate transcription factor binding to nucleosomes independent of their acetylation activity Abstract. Transcription initiation involves the coordination of multiple events, starting with activators binding specific DNA target sequences, which recr

Sorry this is the good link:
academic.oup.com/nar/advance-...

Our collaborative paper is finally published in NAR, gkae1120, doi.org/10.1093/nar/gk…, we reveal that ATAC and SAGA histone acetyl transferase modules (i) acetylate histones to open chromatin AND (ii) facilitate TF targeting within nucleosomes independently of their acetylation activity.

Thanks.