László Tora

Structure of the transcriptional co-activator SAGA complex (from fungus Chaetomium thermophilum), including the histone acetyltransferase module #cryoEM

www.cell.com/molecular-ce...

Enhancer-promoter compatibility is mediated by the promoter-proximal region Gene promoters induce transcription in response to distal enhancers. How enhancers specifically activate their target promoter while bypassing other promoters remains unclear. Here, we find that the p...

What is a promoter? And how does it work?

We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters

www.biorxiv.org/content/10.1...

Nobel Laureate Professor Sir John Gurdon dies aged 92 It is with great sadness that the University shares the news of the death of Professor Sir John Gurdon, founder of the Gurdon Institute.

Today it was announced that Sir John Gurdon passed away.
His ground-breaking discovery was that differentiated cells can be reprogrammed. He was a creative and distinguished developmental biologist, a kind person with a great inquisitive mind and original ideas
www.cam.ac.uk/research/new...

With self-deprecating humor, Sir John loved to tell the story of his Biology teacher (1949)

Novel insights into the structure and evolution of the human SAGA complex by affinity-ligand purification Human SAGA is a 20-subunit complex that stimulates transcription and is essential for development. The most prominent addition to SAGA in metazoans compared to yeast is a 150kDa splicing-factor module...

Read the new version of our manuscript doi.org/10.1101/2025...

FEBS Advanced Lecture Course: 6th Danube Conference on Epigenetics The FEBS Advanced Lecture Course: 6th Danube Conference on Epigenetics will take place 19–22 October 2026 in Budapest, Hungary. Here we give a taste of the scientific content, flag some of the speaker...

Do not forget to apply to the FEBS Advanced Lecture Course: 6th Danube Conference on Epigenetics (19–22 October 2026, Budapest, Hungary. Will be a great meeting as always. Check out the site: network.febs.org/posts/febs-a...

Rethinking RNA-binding proteins: Riboregulation challenges prevailing views RNA-binding proteins (RBPs) are best known as effectors along the entire gene expression pathway and as constituents of RNA-protein machines such as t…

www.sciencedirect.com/science/arti...

"In our view, it [hexanediol] should thus be avoided and not be used as evidence for the formation of an assembly via phase separation or the fluid-like nature of a condensate in vitro or in vivo." doi.org/10.1038/s414...

Cotranslational assembly confers specificity for in vivo target heterodimerization of paralogous H2B-like TAF12 proteins in the human fungal pathogen Candida albicans The fidelity of assembly of multiprotein complexes is essential for the formation of stable and functional protein complexes that are critical for cell growth and survival. In this context, TBP-associ...

Read this interesting preprint from the lab of Krishnamurthy Natarajan: Cotranslational assembly confers specificity for in vivo target heterodimerization of paralogous H2B-like TAF12 proteins in the human fungal pathogen Candida albicans

doi: doi.org/10.1101/2025...

Could you add me please. Thanks. László Tora

One more movie!
Using the RL algorithm (from pnas.org/doi/10.1073/...),
@masaashimazoe.bsky.social analyzed our nucleosome tracking data and classified them by their mobility.
Hotter color = faster motion (Slow🔵→ 🟢 → 🟡 → 🟠 Fast)

Affinity-ligand purification of native human low-abundance multi-protein complexes for structure determination In human cells, large multi-protein transcription co-activators, such as chromatin remodelers or histone acetyltransferases, play critical roles in gene-expression regulation and are often implicated ...

Check out our new collaborative preprint about Affinity-ligand purification of native human low-abundance multi-protein complexes doi.org/10.1101/2025...

Exquisite work. Well done to the whole team.

🚨 New preprint out! Do you think Single Molecule Footprinting and Fiber-seq are super cool but aren't sure how to unlock their full potential? HiddenFoot can help you: www.biorxiv.org/content/10.1...

Friday we celebrated my 40 years of scientific career in France, with present and past lab members and many friends. It was a fantastic day full of emotions, memories and great science. Thanks for making the day so memorable !

The importance of moving away from bulk! Occupancy of Pol II at promoters is dramatically different between fly and mouse cells! When looking single molecule! Proud of the team! @kasitc.bsky.social @molinalab.bsky.social doi.org/10.1038/s443...

Two more weeks to send us your abstract! >20 abstract selected talks. Priority to junior scientists and cool biology on gene regulation (DNA/RNA/protein). Come to meet the world leaders in single molecule genomics and single molecule imaging! Help shaping the future of the field in the workshops!

👍

PMID: 40106549, DOI: 10.1126/sciadv.adr1492

Read our collaborative paper
@longchrom.bsky.social and @molinalab.bsky.social
showing that disruption of NuA4 and ATAC coactivator complexes cause gene-specific transcript buffering, suggesting that cells dynamically adjust RNA export/degradation to sustain cellular homeostasis.

Safeguarding the future of biomedical science in the United States NIH’s abrupt decision to cap indirect cost reimbursement at 15% threatens the critical infrastructure supporting groundbreaking biomedical research in the United States. This policy jeopardizes Americ...

www.cell.com/cell/fulltex...

Friends! The science that we love is under threat. World-wide, there're forces seeking to make it corporate-like, focused on deliverables, or even the enemy of society. Take a stand & celebrate science & music! #theconferencetoendallconferences - Registration starts NOW!
woodstock.img.cas.cz

Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma The midnolin-proteasome pathway degrades many nuclear proteins without ubiquitination, but how it operates mechanistically remains unclear. Here, we present structures of the midnolin-proteasome compl...

Thrilled to share our collaborative work on the structural basis and myeloma disease link of the midnolin-proteasome pathway with Chris Nardone from the Elledge lab, Jingjing Gao from the Shao lab @sshaolab.bsky.social and all the co-authors who put in the hard work! www.biorxiv.org/content/10.1...

‼️ This Wednesday! Don’t forget to register as we host three amazing ECR scientists!
@manucazottes.bsky.social @robertadolling1.bsky.social
@leandrosboukas.bsky.social

⚡️Our recurring registration link:

us06web.zoom.us/webinar/regi...

Intrinsically disordered regions as facilitators of the transcription factor target search - Nature Reviews Genetics Intrinsically disordered domains (IDRs) are increasingly appreciated as important components of protein function. This Perspective discusses the emerging evidence for IDRs from transcription factors a...

Interesting piece on the role of intrinsically disordered regions in #transcriptionfactor specificity, target search etc. ➡️ www.nature.com/articles/s41... #generegulation

Search gets faster, binding longer, bound fraction of cohesin and CTCF goes up, especially at and after ZGA

Increasingly efficient chromatin binding of cohesin and CTCF supports chromatin architecture formation during zebrafish embryogenesis - Nature Communications Using single-molecule tracking of cohesin and CTCF during zebrafish embryogenesis, the molecular kinetics underlying chromatin architecture formation was revealed and gradual emergence of chromatin st...

Single molecules, in living zebrafish embryos, during hour-long period of development: Jonas' work on binding kinetics of cohesin and CTCF around ZGA now online. Great collab with labs of @lucagiorgetti.bsky.social @akispapantonis.bsky.social
doi.org/10.1038/s414...

Gene regulation: one molecule at a time

Program is out for our first EMBL #singlemolecule gene regulation meeting! Check out the dedicated panel sessions on single molecule genomics, single molecule imaging and theory where we would like to brainstorm the future of the field:
Registration is open! 👇
www.embl.org/about/info/c...

The latest publication from our lab is online in @narjournal.bsky.social . Congratulations to @marcelwerner.bsky.social and Manuel Trauner for developing a novel system in mammalian cells to study Transcription-Replication conflicts and pointing DOT1L as a transcription mediator at TRC sites!

CRL3ARMC5 ubiquitin ligase and Integrator phosphatase form parallel mechanisms to control early stages of RNA Pol II transcription Cacioppo, Gillis, Shlamovitz, Zeller, et al. show that CRL3ARMC5 ubiquitylates RNA Pol II at early stages of transcription as part of a major homeostatic RNA Pol II turnover mechanism. CRL3ARMC5 and the Integrator phosphatase act as complementary pathways, monitoring the quantity and quality of RNA Pol II complexes before they are licensed into elongation.

The first paper from our lab is online! A wonderful collaboration with @scottbscience.bsky.social lab. We discover that Pol II is extensively regulated by Ubiquitin at the beginning of the transcription cycle!
www.cell.com/molecular-ce...