Alan Gerber

And it's out!
Our paper on the optimization of smFISH to quantify cytokine mRNAs in T cells is published in EMBO Journal.
Using this protocol, we gathered interesting insights on cytokine mRNAs co-expression and localization in these teeny tiny human cells.
www.embopress.org/doi/full/10....

Congrats Ali!

Programmable control of spatial transcriptome in live cells and neurons - Nature CRISPR-TO, a system for programmable control of spatial localization of cellular RNAs, is presented and enables functional investigation of endogenous RNA localization in diverse living cells.

#spatialbiology #RNAbiology

www.nature.com/articles/s41...

It's out!
Beautiful work led by Loreto Oyarte Gálvez & @cbisot.bsky.social!
Do you want to learn more about how Mycorrhizal fungi extraordinary networks form and function? Read the open-access publication! Link below 👇

I am giving a lecture at the EMBO practical course: "FISHing for RNAs: classical to single molecule approaches"
Check out this hands-on EMBO course!

Excited to share @domenicnarducci.bsky.social 's paper now out in Mol Cell published back-to-back with the paper from @dewitlab.bsky.social‬ - despite lots of prior work, we find that ZNF143 has no looping function
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...

Indeed, featuring the fusion of some letters (but not all) of the first word of the title of a paper we published last year and my last name. Quite an achievement 😅

MDPI & Frontiers journals blacklisted in Finland, I wonder if this will indeed spread further? Never considered any of these journals for publication, but MDPI surely send funny emails... Maybe I will introduce myself like this 👇 from now on 😂.

"We report that numerous “miRNAs” previously reported in EMT and cancer contexts are not incorporated into RISC and are not capable of endogenously silencing target genes, despite the fact that hundreds of publications in the cancer field describe their roles"

www.biorxiv.org/content/10.1...

Transposable element exonization generates a reservoir of evolving and functional protein isoforms Transposable element exonization by unannotated splicing events produces stable protein isoforms with acquired functions that are subject to evolutionary selection.

Not easy to describe the emotions of seeing our work published in @cellpress.bsky.social ! We explored how novel isoforms emerge during evolution through the exonization of Transposable Elements. Exonized TEs modify the functions and structures of host proteins.
www.cell.com/cell/fulltex...

📢We're recruiting📢

Our lab at the University of Lausanne is looking for a postdoctoral associate to study #nucleotide metabolism in physiology and cancer in vivo!

Please share

#uridine #cancermetabolism #immunometabolism

@unil.bsky.social

🔗(PDF): www.jourdainlab.org/wp-content/u...

Structural and kinetic insights into tRNA promoter engagement by yeast general transcription factor TFIIIC Abstract. Transcription of transfer RNA (tRNA) genes by RNA polymerase (Pol) III requires the general transcription factor IIIC (TFIIIC), which recognizes

Excited to share our new work on the earliest steps of Pol-III transcription: We provide structural and kinetic insights into tRNA promoter engagement by yeast transcription factor TFIIIC !

Cool collaboration with the Müller and Eustermann Labs @embl.org !

academic.oup.com/nar/advance-...

Bad bar charts are pervasive across biology Scientific ‘shorthand’ could be introducing data distortions into published papers.

🧪 Your eyes may deceive you. A study of 3,387 life-science papers from 2023 found nearly 1/3 of bar charts distorted data, often by not starting the y-axis at zero or misusing log scales.

Dynamic regulation of RNA Polymerase III transcription in mouse embryonic stem cells during heat shock stress Cells respond to many different types of stresses by overhauling gene expression patterns, both at the transcriptional and translational level. Under heat stress, global transcription and translation ...

🎉Excited to share our new preprint on Biorxiv 🥳
We uncover how RNA Polymerase III (Pol3) dynamically regulates tRNA transcription during heat shock (HS) stress!
So what did we find? 🧵

www.biorxiv.org/content/10.1...

Metabolism and mRNA translation: a nexus of cancer plasticity Tumors often face energy deprivation due to mutations, hypoxia, and nutritional deficiencies within the harsh tumor microenvironment (TME), and as an effect of anticancer treatments. This metabolic st...

Metabolism and mRNA translation: a nexus of cancer plasticity

www.cell.com/trends/cell-...

Paired CRISPR screens to map gene regulation in cis and trans https://www.biorxiv.org/content/10.1101/2024.11.27.625752v1 Recent massively-parallel approaches to decipher gene regulatory circuits have focused on the discov

Paired CRISPR screens to map gene regulation in cis and trans https://www.biorxiv.org/content/10.1101/2024.11.27.625752v1

Epromoters bind key stress-related transcription factors to regulate clusters of stress response genes https://www.biorxiv.org/content/10.1101/2024.11.26.625372v1 Stress insults trigger the rapid and global reprogramming of gene transcription by coordinated recru

Epromoters bind key stress-related transcription factors to regulate clusters of stress response genes https://www.biorxiv.org/content/10.1101/2024.11.26.625372v1

Very cool - snoRNA73 acts as a bridge between mRNAs and 7SL (i.e. the SRP ncRNA) to facilitate the synthesis of the encoded secreted protein.

A Versatile Anti-CRISPR Platform for Opto- and Chemogenetic Control of CRISPR-Cas9 and Cas12 across a Wide Range of Orthologs https://www.biorxiv.org/content/10.1101/2024.11.25.625186v1 CRISPR-Cas technologies have revolutionized life sciences by enabling programmable genome editing ac

A Versatile Anti-CRISPR Platform for Opto- and Chemogenetic Control of CRISPR-Cas9 and Cas12 across a Wide Range of Orthologs https://www.biorxiv.org/content/10.1101/2024.11.25.625186v1

YouTube video by Molecular Biosystems Conference Rachel Green - IUBMB Jubilee Lecture - 3rd Molecular Biosystems Conference (2023)

Did you know that at our conference, we record our Keynote Lectures and make them freely available? You can find them all in our Youtube page!

To get started, check out the 2023 IUBMB Jubilee Lecture, given by Prof. Rachel Green.

Post a non-religious photo you think of as holy.
Not necessarily holy, just cool contrast between "then and now" in ribosome assembly landscape understanding. Nierhaus map from the 80s / AV Broeck & Sebastian Clinge in Science 2023.

Can you add me? Assistant prof at Amsterdam UMC. Thanks!

Could you add me? My group work on regulation of Pol III and tRNAs. Thanks!

Next time I speak with Bob, I'll let him know :-)

This 👇 is happening. Change is coming!

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 by Ueli Laemmli! The paper which introduced SDS-page to the world!!! 💯

Not frog cells, sea urchin embryos

💯

February 14th 1969, RNA polymerases' day.

The pioneering experiment that laid the foundation for the eukaryotic transcription field by Robert Roeder.

Post a non-religious photo you think of as holy

Yes!