Leonardo Almeida-Souza

Great tool. After all, it is not because your manuscript is a pre-print that it does not deserve to be beautifully formatted.

Great opportunity for a Research Support Officer to join @cellbiol-mrclmb.bsky.social, supporting @deriverylab.bsky.social in their studies of the molecular mechanisms behind receptor sorting in endosomes.
More info: www.nature.com/naturecareer...
Apply by 10 JUL
#ScienceJobs

Are you looking for a postdoc?
I will be looking for 2-4 people after the summer to join the lab !!
Do not hesitate to reach out and disseminate !!

Thanks AP.

a cartoon of a man holding a child with the words " you are going to be okay " below him ALT: a cartoon of a man holding a child with the words " you are going to be okay " below him

Wow. A grant and a paper rejection on the same day... I need a beer. And tomorrow, head down and keep going. It is going to be ok.

Thanks!

Yep. I've been there.

The second is far more silly. When I finished figure 1, there was an awkward gap between the graphs. So I found something to fill it. But anyway. I am sure they will not be in the final version of the paper... they are very likely the first to the chop when space reatrictions and revisions kick in.

Fair point. I sometimes wonder if sometimes we go too far. The reasons for the pics are two: first, most people will only open the paper. Scan the figures and move on. The more visual info I give them, the better they may remember the paper in the future and come back for a proper read.

Please let us know if you have any comments on our manuscript or in the technique. We are happy to share the things we have produced and/or to collaborate and help with your protein of interest.
#Share #OpenScience

a man and two women hugging each other with the words `` i love you guys '' . ALT: a man and two women hugging each other with the words `` i love you guys '' .

🧵6/6 — The story behind the manuscript
This work has the crucial contribution of many talented masters students, who helped optimizing various aspects of the system. At the end, An-Sofie, my lab manager and Katja, a postdoc pushed to story until the end.
#CreditWereCreditIsDue

🧵5/6 — The Big Picture
EndoNB works on endogenous proteins. No rare antibodies. No overexpression.
It’s scalable and ready to explore the 1000+ surface proteins we’ve barely studied.
We’re done understanding vesicular trafficking by looking at a handful of model surface proteins.

a drawing of an orange with the words easy peasy lemon squeezed ALT: a drawing of an orange with the words easy peasy lemon squeezed

🧵4/6 — Proof of Concept
With EndoNB, we:
✅ Tracked ligand & antibody effects (Integrin β1, TFR, AXL)
✅ Directly compared receptor uptake (integrin β1 vs β5)
✅ Revealed TMEM123’s endocytic behavior
With easy, clean and reproducible results.

🧵3/6 — The Solution
We CRISPR-tag the protein of interest with a tiny Alfa-tag.
Then use a nanobody-SNAPtag probe with a self-destruct button (okay, a 3C protease site)
Mix them, let internalize, chill at 4°C and use 3C to cut surface signal.
What’s left? Only internalized cargo.

🧵2/6 — The Problem
Cell surfaces are packed with proteins constantly moving in and out.
Studying them? Really hard.
Current tools rely on good antibodies (which are rare) and inefficient signal removal protocols.
So, we built something better.
#CellBiology

🧵1/6 — Preprint Alert!
I am super proud to present the latest work of our lab. Earthlings and those just visiting, I introduce you…. EndoNB: A better, smarter and versatile way to study the dynamics of cell surface proteins. 🧪
www.biorxiv.org/content/10.1...

Nice! Well-deserved. Congratulations, mate.

Hi Rhys. Fyi: I could finally solve the issue. The guys on our HPC cluster found a suitable combination of packages.
Thanks!

Every time I solve one incompatibility, another one appears. I solve it, another appears... and the cycle goes on until the whole thing breaks. I need to use a container to install it ( I cannot use conda), which complicates things. All I need is the set of packages that talk to each other.

I am having many errors. Since the release of the git repo, python, numpy, scipy, tensorflow, biopython, pyrosetta, Jax, cuda, jaxlib, haiku, dm-tree (and others) have newer versions that are either not intercompatible or not compatible with the original scripts.

I have wasted the last week of my life trying to install the RFdiffusion/dl_binder_design pipeline in our HPC cluster. The conflicts with the AF2/JAX/pyrosetta dependencies have driven me insane. The info on the GitHub repo is hugely outdated. Is there a kind soul in Bluesky who can help me? please

Absolutely brutal.

Scientific leaps cannot be taken overnight - Sigrid Jusélius Foundation There are no quick wins in top medical research. Ville Hietakangas, who studies the effect of food on our physiology, asks for patience and trust from those who fund science.

Professor Ville Hietakangas calls for patience from research funders: the time frame in basic research is long, and financial concerns undermine creativity. Read more in our article: sigridjuselius.fi/en/news/scie...

RCF preparing call for universities to invite foreign researchers to Finland The Research Council of Finland (RCF) is planning a new funding call to help universities recruit international experts to Finland. The call would be part of the RCF funding scheme that strengthens un...

Research Council of Finland preparing call for universities to invite foreign researchers to Finland

YES! PIs on the bench. That's how it should be.

Cell and Tissue Dynamics (Wickström)

My lab at the Max Planck Institute for Molecular Biomedicine in Muenster, Germany offers postdoc positions in stem cell mechanobiology. www.mpi-muenster.mpg.de/departments/... drop me an email

The ingredients of a great scientific lecture | EMBO reports EMBO Press is an editorially independent publishing platform for the development of EMBO scientific publications.

'Bob’s true test of a perfectly structured presentation was that the speaker should be able to ditch the PowerPoint slides and successfully deliver the same message using only the blackboard (...) most talks would indeed be better when boiled down to the bare basics: chalk board, presenter, ideas.'

Use the strategy of the fly community and use cool names. Our mumble jumble of letters is not very fun...

Hello Bluesky 👋 We
are #hiring; Calling all aspiring #postdoc s: passionate about #spatialbiology, #proteomics, and the #TME? Join our multidisciplinary team in 🇫🇮 to make impactful discoveries in #ovariancancer
🧬 farkkilab.org/careers/job-ads
Sharing appreciated🤗

This data is very revealing. I have lived in 3 countries of this list ( with widely different stats), and I can see how this simple question explains a lot of people's daily habits.