Nitish Dua

Oxidative phosphorylation complexes contain subunits encoded by both the nuclear and mitochondrial genomes. How do cells coordinate the synthesis and assembly of these complexes at the inner membrane? We teamed up with Martin Ott (@mitolab.bsky.social) to address this in two recent papers.

Just installed Doppio on our Linux machine. This is awesome work making relion more accessible and easy to run. I haven't tried submitting jobs yet but it is already making our live easier!

I met Matt Iadanza at the CZI but I guess he doesn't have bluesky to tag!

An inherited mitochondrial DNA mutation remodels inflammatory cytokine responses in macrophages and in vivo in mice @natcomms.nature.com @ryanlab.bsky.social @tcddublin.bsky.social @universitypress.cambridge.org
www.nature.com/articles/s41...

CryoSift is now out @actacrystf.iucr.org doi.org/10.1107/S205... Thanks to all involved. Happy processing!

Exciting !

I’m excited to announce that my paper describing non-canonical mitotic mechanisms in the early mouse embryo is out in @science.org ! (link at end of 🧵)

StayRose: a photostable StayGold derivative red-shifted by genetic code expansion Photobleaching of fluorescent proteins often limits the acquisition of high-quality images in microscopy. StayGold, a novel dimeric green fluorescent protein recently monomerised through sequence engi...

mStayRose is published in JBC 🥳🎉

Congratulations @wsctt.bsky.social @sporemohan.bsky.social and team for the monumental effort to make a non-natural amino acid incorporating fluorescent protein accessible. It's based on mStayGold, bright, and photostable:

www.jbc.org/article/S002...

Last weeks #immunometabolism discoveries @biomednews.bsky.social ⬇️⬇️⬇️

biomed.news/bims-imicid/...

Lots of excellent work inside incl.

Backtracking metabolic dynamics in single cells predicts bacterial replication in human macrophages.

Structural basis of T-loop–independent recognition and activation of CDKs by the CDK-activating kinase Cyclin-dependent kinases (CDKs) are prototypical regulators of the cell cycle. The CDK-activating kinase (CAK) acts as a master regulator of CDK activity by catalyzing the activating phosphorylation o...

Now out in Science! Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. In @vcushing.bsky.social's magnum opus, we use #cryoEM to figure out how the CDK-activating kinase recognises CDKs to fully activate them - a key step in cell cycle control.
www.science.org/doi/10.1126/...

Mitochondrial transfer

www.nature.com/articles/s42...

The Origin and Diversification of Mitochondria Moving beyond the simplistic view of mitochondria as the power house of the cell, Roger et al. present the immense diversity of mitochondria and mitochondrial functions across eukaryotes, and bring th...

Feeling mitochondrially challenged lately?

Here are two classic reviews on #mitochondria that should help.

www.cell.com/current-biol...

www.cell.com/current-biol...

📣📣📣📣

Bacterial ICMs exhibit internal invaginations that resemble eukaryotic cristae. (A)TEM images showing tubular cristae-like ICM compartments in free-living Desulfobacterota, and similar cristae-like ICM tubules in Desulfovibrio carbinolicus strains. The eukaryotic model organism S. cerevisiae exhibits tubular cristae structures, whereas the commonly used HEK293 cell line exhibits predominantly lamellar cristae structures. Scale bars: 500 nm. (B) Eukaryotic mitochondria tend to exhibit lamellar, tubular or discoidal cristae morphology, whereas orders of the class alphaproteobacteria exhibit a range of ICM morphologies. Lamellar-like ICMs, which exhibit parallel cytoplasmic protrusions that do not contact the inner membrane, are commonly observed in nitrogen-fixing and several methanotrophic alphaproteobacteria. (C) This simplified and non-exhaustive cladogram indicates the two domains of life – bacteria and archaea – with proto-mitochondria emerging in early alphaproteobacteria. Eukaryogenesis is marked by the horizontal transfer (red dotted arrow) from Bacteria to Hordarchaeales in Asgard archaea. §, †, #, * and ‡ connect species listed in B to their respective clades in C.

Kailash Venkatraman, Nicolas-Frédéric Lipp & @ibudin.bsky.social examine the similarities between prokaryotic intracytoplasmic membranes & mitochondrial IMs, & discuss whether cristae evolution has driven specialisation of the #mito lipidome.
journals.biologists.com/jcs/article/...
#JCSMitoSI

I had some absolutely incredible papers come online this week, and I need to take a minute to post about each of them. First, let me tell you about the CarboTag probes for imaging plant cell walls! From the Sprakel lab (a short thread) www.nature.com/articles/s41...

So excited for this work to be out!! Thanks to @attychang.bsky.social, @hamid13r.bsky.social, blue sky less Daniel Fuentes, @nanigrotjahn.bsky.social and @tomo.science!!!

🚨New preprint!🚨 #teamtomo
We expanded Surface Morphometrics to quantify membrane thickness from cryo-ET—revealing local variation across organelles.

Led by the lab’s first grad student, @mmedina300kv.bsky.social (defending Monday! 🍾) w/ @attychang.bsky.social @hamid13r.bsky.social & @tomo.science

#invertefest needs some more marine representation, so here are 2 Liriope sp. hydrozoan and a teeny ctenophore amongst numerous copepods. Data recorded (5 fps) via a benchtop shadowgraph camera from a non-filtering end plankton net tow.

Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social,
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social

🔗 Preprint here tinyurl.com/3a74uanv

I know print journals aren't exactly on everyone's radar these days, but we’re still geeking out that our latest study—led by the brilliant @attychang.bsky.social—landed the cover of the April issue of JCB! 🤩

@zidlab.bsky.social @tomo.science @hamid13r.bsky.social

Live tracking of replisomes reveals nutrient-dependent regulation of replication elongation rates in Caulobacter crescentus Adhikashreni et al. use quantitative live-cell imaging to track cell-cycle dynamics in hundreds of individual bacteria. They observe nutrient-dependent regulation of replication elongation rates that ...

Happy to share Asha Joseph and Inchara Adhikashreni's latest work from the lab, describing replisome speed variation in Caulobacter!

www.cell.com/current-biol...

Retromer promotes the lysosomal turnover of mtDNA Lysosomal uptake of mtDNA controls mitochondrial quality.

Excited to share our latest paper in Science Advances! We finally dissected a new pathway for mitochondrial quality control upon mtDNA replication stress! Let’s start a short thread! 🧵

www.science.org/doi/10.1126/...

Drosophila follicle showing retrotransposons (pink & yellow) expressed in somatic cells infecting the oocyte

1/ Transposable elements are often called "jumping genes" because they mobilize within genomes. 🧬
But did you know they can also jump 𝘣𝘦𝘵𝘸𝘦𝘦𝘯 cells? 🤯
Our new study reveals how retrotransposons invade the germline directly from somatic cells.
www.biorxiv.org/content/10.1...
A short thread 🧵👇

A person in a mask holding a painting that says “hands off my transgenic mice”

At @standupforscience.bsky.social and the signs are statistically significantly excellent

Subtomogram Averaging of a mitochondrially co-translating ribosome

Happy to share that the final version of record for our (
@nanigrotjahn.bsky.social) #teamtomo mitochondrial cotranslation story is online at @jcb.org! We combined STA with surface morphometrics to learn how ribosomes bind to and alter mitochondrial membranes in yeast.

rupress.org/jcb/article/...

Mrx6 binds the Lon protease Pim1 N-terminal domain to confer selective substrate specificity and regulate mtDNA copy number Mitochondrial DNA (mtDNA) copy number regulation remains incompletely understood, despite its importance in cellular function. In Saccharomyces cerevisiae, Mrx6 belongs to the Pet20-domain-containing ...

🚀 Excited to share our latest work on Mrx6 and mtDNA copy number regulation, now available on @biorxivpreprint.bsky.social ! We uncover how Mrx6 and Mam33 interact with the Lon protease Pim1 to control substrate degradation. 🔬 Check it out here: www.biorxiv.org/content/10.1... #mitochondria #mtDNA

Had the incredible opportunity to present my work at #EMBOinsitu workshop in Heidelberg. It was filled with fantastic science and new friends! #teamtomo

When does a DNA polymerase degrade DNA? Read Akshaya’s work from the lab to find out more!

Hey #teamtomo, if you often find yourself using the excellent membrain-seg from @lorenzlamm.bsky.social et al., you might find my napari plugin "napari-segselect" useful. Let's say your tomogram contains the edges of two bacterial cells, each with a membrane and cell wall:

I know, I know... HHS freezes are occupying our attention. Please free up a little bandwidth for this:
nature.com/articles/d4158…
NASA FOUND AN ASTEROID WITH ALL 5 NUCLEOBASES & 14 L-AMINO ACIDS!!
WAIT, THERE'S MORE: THE ASTEROID CONTAINS NEARLY EQUAL AMOUNTS OF D-AMINO ACIDS!

NSF Career Award supported invention of Foldscope - now reached 3 million kids globally with ~1 million kids in US - bringing joy of science to grassroots & communities with very little support. It’s brought communities working together on global problems.

www.goldengooseaward.org/01awardees/f...