joe-wade.bsky.social

🚨The Yap Lab at Northwestern Univ is looking to fill 2 postdoc positions in 2026 to study aspects of Staphylococcus aureus antibiotic resistance and RNA synthesis-degradation. Please contact Frances Yap at frances.yap@northwestern.edu for details. sites.northwestern.edu/yaplab/. Pls repost #Microsky

Phage-encoded sRNA counteracts xenogenic silencing in pathogenic E. coli https://www.biorxiv.org/content/10.1101/2025.11.27.690950v1

Intracellular competition shapes plasmid population dynamics From populations of multicellular organisms to selfish genetic elements, conflicts between levels of biological organization are central to evolution. Plasmids are extrachromosomal, self-replicating g...

Hot off the press! Our latest paper led by @fernpizza.bsky.social, understanding how plasmids evolve inside cells. These small, self-replicating DNA circles live inside bacteria and carry antibiotic resistance genes, but also compete with one another to replicate. 1/
www.science.org/doi/10.1126/...

Brand new preprint from my lab, showing that TnpB, the ancestor of Cas12, acts as a gene drive in plasmids! And it turns out in conjugative plasmids that it acts as a primitive anti-self defense system, providing a potential link between its transposon effect and becoming CRISPR!

Application information for APHL internship

Apply through APHL to be a summer intern at the Wadsworth Center. Both basic science research and public health opportunities available.

The University at Albany Biomedical Sciences Department is hiring at the Assistant Professor level. We are looking for researchers at the intersection of infectious disease and artificial intelligence:
albany.interviewexchange.com/jobofferdeta...
#infectiousdisease #artificialintelligence

Canonical transcription termination mechanisms explain a minority of operons in cyanobacteria https://www.biorxiv.org/content/10.1101/2025.11.10.687660v1

First 🟦 post - my main postdoc work is out in the world! We designed a Cas12a-based genetic screen to identify host factors that contribute to infection by the tickborne pathogen Rickettsia parkeri, and stumbled across a really exciting direct interaction between host and bacterium🦠. Check it out!

Wilkinson Lab We discover and study reverse transcriptases

The Wilkinson Lab is open for science! @mskcancercenter.bsky.social

🧬We'll be finding funky new RNA biology, mainly by looking at reverse transcriptases (i.e. the Best Enzymes In The World)🧬

annnd: I'm hiring - come join! Especially postdocs and PhD students - please get in touch (NYC is great)

De-DUFing the DUFs 🧩 @franznarberhaus.bsky.social lab uncovers how small DUF1127 proteins regulate #phosphate uptake by binding the sensor kinase PhoR. Their conserved role from Agrobacterium to E. coli highlights how even small DUFs can shape bacterial physiology 🦠
buff.ly/jJd9Eho

Thrilled to share our multidisiplinary work on how genome-wide DNA bridging by H-NS reshapes the stationary phase bacterial nucleoid and affects the transcriptional landscape. With Xindan Wang and @meyerroc.bsky.social
www.biorxiv.org/content/10.1...

Research Affiliate I (Post Doctoral) Applications to be submitted by October 13, 2025 Compensation Grade: P99 (Research Affiliate I) Compensation Details: Minimum: $62,353.20 - Maximum: $62,353.20 Annually Positions with a designated wor...

My lab is hiring a postdoc! Official job ad and application instructions here:

healthresearch.wd1.myworkdayjobs.com/en-US/HRI_Ca...

Current deadline says October 13, but this will be extended to be open for ~2 weeks.

grainger birmingham PhD Projects, Programmes & Scholarships - 3 PhDs Listed FindAPhD. Search Funded PhD Projects, Programmes & Scholarships in grainger birmingham. Search for PhD funding, scholarships & studentships in the UK, Europe and around the world.

Our lab is offering 3 PhD projects this year across 3 different topics: antibiotic resistance mechanisms, evolution of pathogenic bacteria, and fundamental mechanisms of gene regulation.

www.findaphd.com/phds/?Keywor...

CRISPR/Cas9-compatible plasmids enabling seven dominant genetic selection methods for the human fungal pathogen Cryptococcus neoformans | Microbiology Spectrum Cryptococcus neoformans is the top-ranked World Health Organization priority fungal pathogen due to its widespread distribution and inadequate treatment options. Additionally, as a basidiomycete yeast...

Small side-project from my postdoctoral work with @hitenmadhani.bsky.social. We found that blasticidin S can select for Cryptococcus transformants, and made CRISPR-compatible plasmids for other existing markers. Now 7 dominant genetic selection methods in total!

journals.asm.org/doi/10.1128/...

Our lab website is live, check it out: pokorzynski-lab.squarespace.com

The Tufts IRACDA Program is now accepting applications. The deadline for positions beginning in late fall/early winter 2025–2026 is October 15, 2025. For details, please visit gsbs.tufts.edu/academics/tu...
I am also happy to discuss potential fellowship projects in my lab.

NOT TO BE MISSED. My awesome colleague Rob Brewster may have COMPLETELY upended our understanding of how TFs work ... a must-read for anyone who cares in the slightest about gene regulation

Translational coupling of neighboring genes in prokaryotes | Journal of Bacteriology Prokaryotic genes are arranged in operons, with functionally related genes often located adjacent to one another (1). There are several ways in which the operonic organization of genes facilitates the...

Check out our new paper: a review of translational coupling, the phenomenon where translation of one prokaryotic gene can promote translation of the gene downstream. We cover the history, and delve into the mechanism, which is still not fully understood. journals.asm.org/doi/10.1128/...

Great scientific discussions and community at the Regulating with RNA in Bacteria and Archaea Meeting in Strasbourg 🇫🇷, presenting NIAID research on B. burgdorferi sRNAs and single-cell RNA-seq #rna

Looking for a new approach to studying or eliminating phages? Check out our study introducing anti-phage ASOs (antisense oligos) out in @Nature today. nature.com/articles/s4158…

De novo engineered guide RNA-directed transposition with TnpB-family proteins reveals features of naturally evolved systems Programmable DNA integration using CRISPR-associated transposons (CASTs) offers powerful capabilities for genome engineering. The single effector Cas12k CAST examples evolved from a fixed guide TnpB n...

De novo engineered guide RNA-directed transposition with TnpB-family proteins reveals features of naturally evolved systems www.biorxiv.org/content/10.1...

Widespread purine bias in bacterial genes driven by runaway transcription https://www.biorxiv.org/content/10.1101/2025.08.25.672197v1

🚨 New Preprint Alert 🚨

Our latest work, A Zur-dependent regulatory RNA involved in maintaining zinc homeostasis in Staphylococcus aureus, is now live on bioRxiv! 🧬🦠📄

Check it out here: www.biorxiv.org/content/10.1...

Analysis of stress-induced small proteins in Escherichia coli reveals that YoaI mediates cross-talk between distinct signaling systems Bacterial small proteins (≤ 50 amino acids) are an emerging class of regulators that modulate the activity of signaling networks that enable bacterial adaptation to stress. The Escherichia coli genome...

Analysis of stress-induced small proteins in Escherichia coli reveals that YoaI mediates cross-talk between distinct signaling systems 🦠 from Yadavalli lab. Ribo-seq study of Mg2+ starvation induced 17 small proteins 💫 #RNAsky #microsky #ribosome #smallproteins doi.org/10.1101/2024...

PIRT-Seq: a high-resolution whole-genome assay to identify protein-coding genes Abstract. The advent of high-density mutagenesis and data-mining studies suggest the existence of further coding potential within bacterial genomes. Small

Out yesterday. Using a translation reporter on a mini-tn5 transposon, and high freq. mutagenesis, we looked at translation on whole genome scale independently of genome annotation. academic.oup.com/nar/article/...

Comprehensive mapping of transcription terminator Rho utilization (Rut) sites across the Bacillus subtilis genome Abstract. Recent evidence indicates that the bacterial Rho helicase regulates Bacillus subtilis gene expression in a growth-dependent manner. This regulati

New cool work from French friends and colleagues: identification of Rho termination sites in B. subtilis! academic.oup.com/nar/article/...

#SubtiWiki @narjournal.bsky.social @inrae-france.bsky.social

Talk slots full but we can take late breaking poster abstracts until August 7th. Hope to see you in York to hear about your chromosome story 🧬🦠🧫

www.eventsforce.net/biochemsoc/f...

@vslioy.bsky.social @damelab.bsky.social @graingerlab.bsky.social @biochemsoc.bsky.social @biophychrom.bsky.social

Our work to functionally map the Cryptococcus genome is now "officially" out in Cell!

Had a fabulous day at the Wadsworth Center in Albany yesterday 🤩 Very impressive place to do science plus everybody is so kind 🤗

Thanks so much Sian @sianowen.bsky.social and Tom Bartlett for the kind invitation 🤩. Glad to see your new labs started so well.

#RNASky #ribosome 🧪