joe-wade.bsky.social

Research Affiliate I (Post Doctoral) Applications to be submitted by October 13, 2025 Compensation Grade: P99 (Research Affiliate I) Compensation Details: Minimum: $62,353.20 - Maximum: $62,353.20 Annually Positions with a designated wor...

My lab is hiring a postdoc! Official job ad and application instructions here:

healthresearch.wd1.myworkdayjobs.com/en-US/HRI_Ca...

Current deadline says October 13, but this will be extended to be open for ~2 weeks.

grainger birmingham PhD Projects, Programmes & Scholarships - 3 PhDs Listed FindAPhD. Search Funded PhD Projects, Programmes & Scholarships in grainger birmingham. Search for PhD funding, scholarships & studentships in the UK, Europe and around the world.

Our lab is offering 3 PhD projects this year across 3 different topics: antibiotic resistance mechanisms, evolution of pathogenic bacteria, and fundamental mechanisms of gene regulation.

www.findaphd.com/phds/?Keywor...

CRISPR/Cas9-compatible plasmids enabling seven dominant genetic selection methods for the human fungal pathogen Cryptococcus neoformans | Microbiology Spectrum Cryptococcus neoformans is the top-ranked World Health Organization priority fungal pathogen due to its widespread distribution and inadequate treatment options. Additionally, as a basidiomycete yeast...

Small side-project from my postdoctoral work with @hitenmadhani.bsky.social. We found that blasticidin S can select for Cryptococcus transformants, and made CRISPR-compatible plasmids for other existing markers. Now 7 dominant genetic selection methods in total!

journals.asm.org/doi/10.1128/...

Our lab website is live, check it out: pokorzynski-lab.squarespace.com

The Tufts IRACDA Program is now accepting applications. The deadline for positions beginning in late fall/early winter 2025–2026 is October 15, 2025. For details, please visit gsbs.tufts.edu/academics/tu...
I am also happy to discuss potential fellowship projects in my lab.

NOT TO BE MISSED. My awesome colleague Rob Brewster may have COMPLETELY upended our understanding of how TFs work ... a must-read for anyone who cares in the slightest about gene regulation

Translational coupling of neighboring genes in prokaryotes | Journal of Bacteriology Prokaryotic genes are arranged in operons, with functionally related genes often located adjacent to one another (1). There are several ways in which the operonic organization of genes facilitates the...

Check out our new paper: a review of translational coupling, the phenomenon where translation of one prokaryotic gene can promote translation of the gene downstream. We cover the history, and delve into the mechanism, which is still not fully understood. journals.asm.org/doi/10.1128/...

Great scientific discussions and community at the Regulating with RNA in Bacteria and Archaea Meeting in Strasbourg 🇫🇷, presenting NIAID research on B. burgdorferi sRNAs and single-cell RNA-seq #rna

Looking for a new approach to studying or eliminating phages? Check out our study introducing anti-phage ASOs (antisense oligos) out in @Nature today. nature.com/articles/s4158…

De novo engineered guide RNA-directed transposition with TnpB-family proteins reveals features of naturally evolved systems Programmable DNA integration using CRISPR-associated transposons (CASTs) offers powerful capabilities for genome engineering. The single effector Cas12k CAST examples evolved from a fixed guide TnpB n...

De novo engineered guide RNA-directed transposition with TnpB-family proteins reveals features of naturally evolved systems www.biorxiv.org/content/10.1...

Widespread purine bias in bacterial genes driven by runaway transcription https://www.biorxiv.org/content/10.1101/2025.08.25.672197v1

🚨 New Preprint Alert 🚨

Our latest work, A Zur-dependent regulatory RNA involved in maintaining zinc homeostasis in Staphylococcus aureus, is now live on bioRxiv! 🧬🦠📄

Check it out here: www.biorxiv.org/content/10.1...

Analysis of stress-induced small proteins in Escherichia coli reveals that YoaI mediates cross-talk between distinct signaling systems Bacterial small proteins (≤ 50 amino acids) are an emerging class of regulators that modulate the activity of signaling networks that enable bacterial adaptation to stress. The Escherichia coli genome...

Analysis of stress-induced small proteins in Escherichia coli reveals that YoaI mediates cross-talk between distinct signaling systems 🦠 from Yadavalli lab. Ribo-seq study of Mg2+ starvation induced 17 small proteins 💫 #RNAsky #microsky #ribosome #smallproteins doi.org/10.1101/2024...

PIRT-Seq: a high-resolution whole-genome assay to identify protein-coding genes Abstract. The advent of high-density mutagenesis and data-mining studies suggest the existence of further coding potential within bacterial genomes. Small

Out yesterday. Using a translation reporter on a mini-tn5 transposon, and high freq. mutagenesis, we looked at translation on whole genome scale independently of genome annotation. academic.oup.com/nar/article/...

Comprehensive mapping of transcription terminator Rho utilization (Rut) sites across the Bacillus subtilis genome Abstract. Recent evidence indicates that the bacterial Rho helicase regulates Bacillus subtilis gene expression in a growth-dependent manner. This regulati

New cool work from French friends and colleagues: identification of Rho termination sites in B. subtilis! academic.oup.com/nar/article/...

#SubtiWiki @narjournal.bsky.social @inrae-france.bsky.social

Talk slots full but we can take late breaking poster abstracts until August 7th. Hope to see you in York to hear about your chromosome story 🧬🦠🧫

www.eventsforce.net/biochemsoc/f...

@vslioy.bsky.social @damelab.bsky.social @graingerlab.bsky.social @biochemsoc.bsky.social @biophychrom.bsky.social

Our work to functionally map the Cryptococcus genome is now "officially" out in Cell!

Had a fabulous day at the Wadsworth Center in Albany yesterday 🤩 Very impressive place to do science plus everybody is so kind 🤗

Thanks so much Sian @sianowen.bsky.social and Tom Bartlett for the kind invitation 🤩. Glad to see your new labs started so well.

#RNASky #ribosome 🧪

The Mycobacterium tuberculosis Transposon Sequencing Database (MtbTnDB): A Large‐Scale Guide to Genetic Conditional Essentiality Mtb transposon sequencing database is a unified resource for exploring conditional gene essentiality in Mycobacterium tuberculosis. By standardizing over 150 transposon sequencing screens, it enables...

Jinich et al. present an important new resource for the Mtb community!
The Mycobacterium tuberculosis Transposon Sequencing Database (MtbTnDB) is an interactive web resource summarizing 150+ published screens.
#MicroSky #Mycobacterium #tuberculosis
onlinelibrary.wiley.com/doi/10.1111/...

The Wadsworth Center, New York State Department of Health, is currently recruiting for a Research Scientist 5 (Assistant/Associate Professor equivalent) to lead an independent research program focused on zoonotic and/or vector-borne pathogens (viral and bacterial).

The Wadsworth Center is expanding it's research focus on #virology /vector-borne diseases!
www.asmcareerconnections.org/job/research-scientist-5-g-31/78488664/
This is a unicorn assistant prof equivalent position with 12-month hard money salary and no teaching commitments (!)
Reposts appreciated 🙏

Complementary Ribo-seq approaches map the translatome and provide a small protein census in the foodborne pathogen Campylobacter jejuni - Nature Communications Bacterial genomes still contain many hidden and uncharacterized genes. Using complementary Ribo-seq approaches, Froschauer, Svensson et al. here annotate start and stop codons of Campylobacter jejuni, reporting a high-resolution translatome and identifying several novel small ORFs in this foodborne pathogen.

Bacterial genomes still contain many hidden genes.
This article reports a high-resolution map of translated proteins, incl. small ones, in the pathogen Campylobacter jejuni

@uniwuerzburg.bsky.social @campy-bara.bsky.social @sharmalab.bsky.social

Disco lights

Phage DisCo! Our targeted phage discovery method in press today: journals.asm.org/doi/10.1128/...

Perfectly timed with our four summer undergrad's first successful phage hunt... disco lights in the lab to mark the occasion 🕺

Mycobacterium tuberculosis MutT4 is an RNA pyrophosphohydrolase that forms biomolecular condensates and sensitizes mRNAs to degradation https://www.biorxiv.org/content/10.1101/2025.05.13.653832v1

Really excited to share that I'll start my research lab at the Wadsworth Center with the New York State Department of Health in August 2025! My group will use functional genomics to study how fungal pathogens have repurposed machinery from their environmental lifestyles for mammalian infection!

A bacterial CARD-NLR immune system controls the release of gene transfer agents Bacteria have evolved a wide array of immune systems to detect and defend against external threats including mobile genetic elements (MGEs) such as bacteriophages, plasmids, and transposons. MGEs are ...

1/ Excited to share our latest work on gene transfer agents (GTAs) in Caulobacter crescentus, led by Emma Banks in collaboration with Pavol Bardy and Mai Nguyen from York!!! See a brief thread below.
shorturl.at/o6S1w

The small membrane protein YohP induces membrane depolarization and ppGpp accumulation in Escherichia coli. https://www.biorxiv.org/content/10.1101/2025.05.06.652344v1

Stenotrophomonas maltophilia exhibits defensive multicellularity in response to a Pseudomonas aeruginosa quorum sensing molecule Microorganisms commonly exist in polymicrobial communities, where organisms can respond to interspecies secreted molecules by altering behaviors and physiology, however, the underlying mechanisms rema...

New preprint alert 🚨: We found that Steno maltophila senses a quorum sensing molecule from the co-infecting pathogen Pseudomonas aeruginosa and switches to multicellular aggregates as a protective mechanism. #MicroSky
www.biorxiv.org/content/10.1...

Modulation of protein activity by small RNA base pairing internal to coding sequences Thongdee et al. reveal that regulatory RNA pairing internal to coding sequences can lead to locally slowed translation elongation and increased enzymatic activity, likely through effects on co-transla...

Modulation of protein activity by small RNA base pairing internal to coding sequences: Molecular Cell www.cell.com/molecular-ce...