Albert Dominguez Mantes

Now speaking #CBIAS2025 @albertdm.bsky.social from @maweigert.bsky.social introducing spotiflow www.nature.com/articles/s41...

Had a blast at #CBIAS2025! Great science (like the incredible ShapeEmbed talk from @afoix.bsky.social), amazing people and obviously tons of beautiful images :) Already looking forward to coming back next year!

We’ve upgraded ShapeEmbed 🎉 ShapeEmbedLite decodes latent codes via an MLP to guarantee valid EDMs, making it lighter and ideal for small microscopy datasets or limited compute. Hear more at my BIC workshop talk or poster at #ICCV2025! Try it out at github.com/uhlmanngroup...

🧠 The Lipid #Brain Atlas is out now! If you think #lipids are boring and membranes are all the same, prepare to be surprised. Led by @lucafusarbassini.bsky.social with Giovanni D'Angelo's lab, we mapped membrane lipids in the mouse brain at high resolution.
www.biorxiv.org/cgi/content/...

You may have heard me talk about learned shape representations for a long time, but it took @afoix.bsky.social's hard work to bring it to life 🍾 Catch her at @neuripsconf.bsky.social to find out more!!

Happy to share that ShapeEmbed has been accepted at @neuripsconf.bsky.social 🎉 SE is self-supervised framework to encode 2D contours from microscopy & natural images into a latent representation invariant to translation, scaling, rotation, reflection & point indexing
📄 arxiv.org/pdf/2507.01009 (1/N)

(1/14) I’m happy and proud to introduce: SpinePy – a framework to detect the "spine" of gastruloids and measure biological and physical signals in a local dynamic 3D coordinate system. www.biorxiv.org/content/10.1...

Unified mass imaging maps the lipidome of vertebrate development Nature Methods - uMAIA is an analytical framework designed to enable the construction of metabolic atlases at high resolution using mass spectrometry imaging data.

Happy to share our work on uMAIA, a framework for building metabolomic atlases from mass spectrometry imaging.
With uMAIA, we mapped the lipidome of zebrafish development, uncovering spatially organized metabolic programs .
www.nature.com/articles/s41...
#developmentalbiology #MSI #lipidtime

QuPath extension Spotiflow is now available Hello @qupath team & users, We are pleased to announce a new qupath-spotiflow-extension to handle spotiflow in QuPath 🎉 ! This extension reproduces the logic behind the qupath-extension-cellpose to ...

A new extension! Rémy made a wrapper of @albertdm.bsky.social + @maweigert.bsky.social 's Spotiflow (2/3)

forum.image.sc/t/qupath-ext...

Cell tracking is never perfect, and it's important to understand the types of errors your solution contains.

Here is my stab at this: Divisualisations in @napari.org. github.com/bentaculum/d...

You spin tracks out upwards from the playing video. Green edges are correct, FP in magenta, FN in cyan.

Working in Martin’s group is an amazing experience, don’t hesitate to apply and reach out if you have any questions!

Out today in @natmethods.nature.com : Spotiflow, our transcript localization method for imaging-based spatial transcriptomics. Led by amazing PhD student @albertdm.bsky.social, joint work w @gioelelamanno.bsky.social at EPFL / @scadsai.bsky.social
www.nature.com/articles/s41...
rdcu.be/epIB7

Spotiflow: accurate and efficient spot detection for fluorescence microscopy with deep stereographic flow regression - Nature Methods Spotiflow uses deep learning for subpixel-accurate spot detection in diverse 2D and 3D images. The improved accuracy offered by Spotiflow enables improved biological insights in both iST and live imag...

A nice advance for imaging-based spatially resolved transcriptomics from the Weigert and La Manno labs. Spotiflow uses deep learning for subpixel-accurate spot detection in diverse 2D and 3D images. www.nature.com/articles/s41...

A massive thanks to all the incredible people who contributed to this work, it's been an amazing ride! And a shoutout to all the people who have tried already tried Spotiflow and provided feedback, it's truly great to see the community using it :)
(N/N)

GitHub - weigertlab/spotiflow: Accurate and efficient spot detection for microscopy data Accurate and efficient spot detection for microscopy data - weigertlab/spotiflow

For more details on using Spotiflow, check our repository at github.com/weigertlab/s...! We are very excited to see what uses cases people will find for Spotiflow!
(9/N)

Results showing the effect of the number of training images on model performance for training from scratch and fine-tuning

We also offer several out-of-the-box pre-trained 2D/3D models. Fine-tuning a model on your own data is easy and accessible if you're not satisfied with the pre-trained ones, and we show Spotiflow is not particularly data hungry, so no need to spend a lot of time annotating!
(8/N)

We developed Spotiflow with the mindset of making it useful and usable, and as such it comes in different flavors: one can use it as a Python API, as a CLI or with a GUI using our @napari.org plugin! Hopefully more integrations will come soon ;)
(7/N)

Overview of EASI-FISH data + pairs of insets containing raw (left) and Spotiflow detections (right)

To be ready to handle today’s massive spatial biology data, we built a multi-GPU setup that e.g. processes a cloud-stored 159 GB EASI-FISH volume in under 10 minutes on 8×A100 GPUs. This can easily be deployed seamlessly on any modern compute cluster!
(6/N)

In a single molecule tracking experiment w/ Eftychia Kyriacou and Joachim Lingner from EPFL, Spotiflow allowed us to improve results by changing the input detections to Trackmate, showing that Spotiflow can be used as a drop-in replacement in existing workflows!
(5/N)

The context-awareness of Spotiflow allows for precise detection in challenging SNR conditions. This includes avoiding artifacts due to e.g. autofluorescence as we show with the beautiful P. dumerilii volumetric smFISH data from the Arendt and @ilastik-team.bsky.social teams @embl.org
(4/N)

Benchmarking results for different spot detection methods

Spotiflow achieves SOTA results on several datasets (iST, live imaging, DNA FISH) while being faster than commonly-used spot detection methods when used out-of-the-box on large-scale data, both in 2D and 3D!
(3/N)

Given an image or volume, Spotiflow uses a neural network to predict multiscale Gaussian heatmaps and the “stereographic flow” — a smooth embedding that maps the nD nearest-spot vector field to the (n+1)D sphere, avoiding issues at infinity and enabling precise localization
(2/N)

Spotiflow: accurate and efficient spot detection for fluorescence microscopy with deep stereographic flow regression Nature Methods - Spotiflow uses deep learning for subpixel-accurate spot detection in diverse 2D and 3D images. The improved accuracy offered by Spotiflow enables improved biological insights in...

Spotiflow, our deep learning based spot detection method for microscopy, is now published in @natmethods.nature.com!
Since the pre-print, we have added many features, notably native 3D detection!
@maweigert.bsky.social @gioelelamanno.bsky.social @epfl-brainmind.bsky.social
Paper: rdcu.be/epIB7
(1/N)

AI Method Improves Transcript Detection in Microscopy Images Spotiflow improves large-scale transcript localization by combining deep learning with a novel geometric detection approach.

Get to know Spotiflow, an AI method that improves large-scale transcript localization by combining deep learning with a novel geometric detection approach.

Learn more:
👉 scads.ai/transcript-d...

Find the publication in Nature Methods:
👉 www.nature.com/articles/s41...

AI@MBL: Machine Learning for Microscopy Image Analysis | Marine Biological Laboratory The goal of this course is to familiarize researchers in the life sciences with state-of-the-art deep learning techniques for microscopy image analysis and to introduce them to tools and frameworks th...

How can AI help you to analyze your microscopy images?

Find out at the "Machine Learning for Microscopy Image Analysis" course at the @mblscience.bsky.social in Woods Hole from Aug 22-Sep 6!

Applications are due 🚨 April 30 🚨

Read on for more! 👇

www.mbl.edu/education/ad...

🔬🎤 As pre-announced a few days ago... please let us proudly present to you: 𝑴𝒊𝒄𝒓𝒐𝕊𝒑𝒍𝒊𝒕 - your ticket to imaging more, imaging more gentle, and/or imaging more efficient. 🔬

doi.org/10.1101/2025...

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