Logan Myler

Sounds good! We also use 20% methanol and 0.02% SDS so maybe that’ll do the trick. Always happy to help!

06.12.2024 11:38 — 👍 1    🔁 0    💬 1    📌 0

We have both in the lab, but I haven’t tried the HMW stuff with the fancy fast transfer (although it says that it should be able to handle it). 2 hours in the cold room 400mA. You’re welcome to take a couple gels if you want to try them to rule out if it’s tris-glycine or just those specific gels

05.12.2024 20:01 — 👍 1    🔁 0    💬 1    📌 0

What are your plans for October 2025? It would be a fantastic time to visit Norway for the Endogenous DNA Damage and Repair conference with a stellar set of speaker covering a wide range of topics. www.endodnadamage.com

04.12.2024 11:14 — 👍 53    🔁 20    💬 3    📌 5

I have 4-12% tris-glycine - in Titia’s lab we never bought the 4-20% tris glycine ones for some reason but maybe this is why? I have no idea why the 4-20 specifically would have a problem

05.12.2024 13:54 — 👍 1    🔁 0    💬 1    📌 0

I haven’t had any problem transferring a 350kDa protein with the 4-12% tris-glycine gels for 2 hours at 400mA - have you tried 4-12% gels? I have some if you want to try it

05.12.2024 03:29 — 👍 1    🔁 0    💬 1    📌 0

Delighted to publish my new molecular animation:

DNA Break Repair by Homologous Recombination

youtu.be/Xe-83tBcxhs

04.12.2024 00:07 — 👍 264    🔁 112    💬 37    📌 40