Makaía Papasergi-Scott

You want to build an apparatus for time-resolved cryo-EM?
Easy:
bio-protocol.org/en/bpdetail?...

Happy New Year! (and first post here 😀) Excited to share the final version of our work where we combine deep mutational scanning, cryo-EM, and molecular dynamics to further resolve the mechanisms of pH sensing in GPCRs.

🧵(1/n)

www.cell.com/cell/fulltex...

Elucidating the dynamics of Integrin αIIb[beta]3 from native platelet membranes by cryo-EM with build and retrieve method https://www.biorxiv.org/content/10.1101/2024.11.27.625729v1 Platelets fulfill their essential physiological roles sensing the extracellular environment through

Elucidating the dynamics of Integrin αIIb[beta]3 from native platelet membranes by cryo-EM with build and retrieve method https://www.biorxiv.org/content/10.1101/2024.11.27.625729v1

Very excited to share the first story from my postdoc where we uncover the structural basis for slow Cas9 turnover!

We use kinetics-guided cryo-EM to capture 25 conformational states of SpCas9 from a single dataset - including the first look at Cas9 releasing product.

biorxiv.org/content/10.1...

Full Circle Native symposium flyer. It includes a Logo, the date of the event (April 3-4 2025), and confirmed speakers: Casey Dorr (Hennepin Healthcare Research Institute), Rebecca Pollet (Vassar College), Kat MIlligan-McClellan (University of Conneticut), Krystal Tosie (Arizona State University), Lauren W Yowelunh McLester-Davis (UW Madison). It also includes that text: The goal of the “Full Circle” symposium is to create an opportunity for North American Native and Indigenous, Native Hawaiian, and Alaskan Native scientists to share their research, meet other scientists, and build community. The symposium will take place over two days at the UCSF Mission Bay campus in San Francisco. We will have talks from students, postdocs, and faculty and panel discussions on Native and Indigenous topics in the context of research. It also contains a QR code that links to: https://forms.gle/AN7nfkhdB3Fvdkkm7

We will be hosting 3rd Full Circle Symposium for Native Biologists hosted at UCSF in April 2025! We aim to build community and provide meaningful opportunities for Native scientists. If you are a Native biologist who is interested plz fill this out to stay up to date: forms.gle/AN7nfkhdB3Fv...

Improved automated model building for cryo-EM maps using CryFold https://www.biorxiv.org/content/10.1101/2024.11.13.623164v1 Constructing atomic models from cryogenic electron microscopy (cryo-EM) density maps is essential fo

Improved automated model building for cryo-EM maps using CryFold https://www.biorxiv.org/content/10.1101/2024.11.13.623164v1

Brass microscope in mendel‘s bedroom with a silly little fur hat on it

Mendel’s other brass microscope with a silly little hat on it, this one has a tassel

as a Big Nerd I have to tell you I went to Mendel’s abbey to look at his peas but the most notable part is actually the little hats he made for his microscopes

A profile picture of Douglas Prasher from Martin Chalfie's Nobel Lecture

Do you know who Douglas Prasher is? Many don't, even though he is the person who cloned the original #GFP gene in the late 1980s. In my short history of plant light #microscopy I also cover a bit of his story - & why he is relatively unknown today, despite the importance of his work. See this 🧵👇

Cryo em density where there are clear signs of manipulation

Slices of density clearly showing certain artificial manipulations

Text that reads : to repeat the point our published work has gained full acceptance by credible, academic peers and major journals. He has not proven any major inaccuracies in our work that has made sufficient noise that few journals have erroneously responded by retracting a small number of publications.

Highlighted text : the junior researcher who set off this fire storm is making vicious accusations, but he has never published. Peer reviewed findings that contradict my labs work. He is simply and repeatedly attacked us while ranting his opinions.

A certain researcher has had 3 papers retracted due to obvious manipulation as in this example below. Saying that scientists cannot push for retraction because a paper is already peer reviewed or should shut up as they never tried to republish something completely wrong is not a good look

Thanks for putting this together! Please add me :)

A first attempt to assemble GPCR aficionados here on 🦋 Let me know who I missed by replying below!

go.bsky.app/75ukb81

I am already running a service to notify about new #SerialEM releases by email at nexperion.net/serialemupdatenotices, but considering to make this a Bluesky bot as well ... is this something the #teamtomo #cryoEM #cryoET community would be interested in?

Thanks for sharing! Please add me :)

I kind of forgot how to announce Feeds, but here goes

For an all-in-one CryoEM Feed:

#CryoEM, #CryoET, #TeamTomo all-in-one feed that curates posts from the last 7 days containing the various cryoEM and cryoET related hashtags and keywords.

bsky.app/profile/did:...

Promiscuous G-protein activation by the calcium-sensing receptor - Nature Structures of the human calcium-sensing receptor can be bound into complex with G proteins from three different Gα subtypes while maintaining G-protein-binding specificity.

www.nature.com/articles/s41...

Time-resolved cryo-EM of G-protein activation by a GPCR - Nature Time-resolved cryo-EM is used to capture structural transitions during G-protein activation stimulated by a G-protein-coupled receptor.

"Time-resolved cryo-EM of G protein activation by a GPCR" #CryoEM #membrane #protein #structure #GPCR www.nature.com/articles/s41...

This one is for the #cryoEM afficionados. Ten years ago tomorrow (March 14th, 2014), I was at the Tahoe symposium on cryoEM 3D image analysis (taking place again this year, starting today!) and attended a talk by Alberto Bartesaghi, then from NCI-NIH now Duke.

Interplay between Mg2+ and Ca2+ at multiple sites of the ryanodine receptor https://www.biorxiv.org/content/10.1101/2024.02.16.580716v1 RyR1 is an intracellular Ca2+ channel important in excitable cells. Ca2+ activates it at low concent

Interplay between Mg2+ and Ca2+ at multiple sites of the ryanodine receptor https://www.biorxiv.org/content/10.1101/2024.02.16.580716v1

Super cool study on the nicotinic receptor using time-resolved cryoEM! www.cell.com/cell/fulltex...

Some rings for #FluorescenceFriday: spectrin labeling of the axonal periodic scaffold seen by 3D-STORM. Did you know that rings are synchronized between axons when bundle together? Now you do!

Here's an exciting job for the right person.

Chance to join the senior staff at EMBO.

Based in Heidelberg at the EMBO headquarters, it's a great organisation and a great place to work.

www.embl.org/jobs/positio...

Read the Community guidelines for GPCR ligand bias: IUPHAR review 32: https://bpspubs.onlinelibrary.wiley.com/doi/10.1111/bph.15811Browse the British Journal... Community guidelines for GPCR ligand bias: IUPHAR review 32 - interview with Prof David Gloriam

Enlightening interview with David Gloriam about how to properly measure GPCR ligand bias.
youtube.com/watch?v=g5PX...

www.sciencedirect.com/science/arti...
Our Cell paper appeared today:
"Time resolution in cryo-EM using a PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling" --
New chip, described in detail + biol. application: intermediates at 10, 25, 140 ms.

In the first issue of the year, we announce we are using the program Proofig to analyze papers for images that may be been inappropriately altered. In our testing, most authors were very grateful to get the reports. More in my column: www.science.org/doi/10.1126/...

Transitioning from tweets, NASW migrates across to Bluesky NASW is transitioning to Bluesky as its short-form social media platform for community building. Find out how NASW members can join the conversation.

Science Writers: NASW is transitioning from Twitter to Bluesky as its short-form social media platform for community building. To facilitate Bluesky signups for our member network, we've created a code donation and receiving tool. See you all here soon! www.nasw.org/article/nasw... 💚

a children’s book called “PIs for Pancakes”

brunch with your boss

Molecular determinants of ligand efficacy and potency in GPCR signaling www.science.org/doi/10.1126/...

About: SerialEM version 4.1.0

Not sure how many SerialEM users in #cryoEM and #cryoET are already here, but: SerialEM 4.1 was just released. It comes with many useful new features and script commands, thanks to the tireless work of David Mastronarde bio3d.colorado.edu/ftp/SerialEM...