Jakub Kopycinski

Cytokines look “modest”. I can do this all day, but would no-doubt offend people.

Loving the CD4 vs CD8.

I see that and raise you this:

Lemmeguess, belongs to an overconfident medic in the 1st year of their PhD?

Pre-print alert! And this one really is a must read for anyone that does spectral #flowcytometry. It is a complete, fully-automated spectral unmixing pipeline that reduces error up to 9000-fold.

Want to get a tough sample, like lung, looking like this? Read more!

www.biorxiv.org/content/10.1...

Hey Colossal - spend some of your money on saving this real bird!

An interdisciplinary study uncovers how benign borderline ovarian tumors develop into invasive low-grade serous carcinoma, opening a new avenue for targeted therapy.

❕Publication: www.cell.com/cancer-cell/...

❕Press Release: www.biochem.mpg.de/when-tumors-...

Memory CD8+ T cells have differential cytotoxic capacities across human tissues @sciimmunology.bsky.social
www.science.org/doi/10.1126/...

A microfluidic chip-based antigen-specific T-cell response assay (ASTRA) that automates the detection of M.tb-specific T-cell activation responses to facilitate screening for latent M.tb infection and TB
www.nature.com/articles/s41...
‪@natbiomedeng.nature.com‬

www.vulture.com/article/zohr...

I've just signed "Stop the Oxford Congestion Charge" - Will you support the campaign too? actionstorm.org/petitions/st...

An Il12 mRNA-LNP adjuvant enhances mRNA vaccine–induced CD8 T cell responses IL-12 is dispensable for CD8 T cell responses to mRNA vaccination, but an Il12 mRNA-LNP enhances CD8 T cell memory and protection.

mRNAs encoding adjuvant proteins boost immunity. A fantastic study from Hunter et al shows the value of IL12. Study validates original work on this concept, ref below.
Kudos to the authors on this exciting discovery!
www.science.org/doi/10.1126/...

ABSTRACT A leading HIV vaccine strategy requires a priming immunogen to induce broadly neutralizing antibody (bnAb) precursors, followed by a series of heterologous boosters to elicit somatic hypermutation (SHM) and produce bnAbs. In two randomized, open-label phase 1 human clinical trials, IAVI-G002 in the United States and IAVI-G003 in Rwanda and South Africa, we evaluated the safety and immunogenicity of mRNA-encoded nanoparticles as priming immunogens (both trials) and first-boosting immunogens (IAVI-G002). The vaccines were generally safe and well tolerated, except 18% of IAVI-G002 participants experienced skin reactions. Priming induced bnAb precursors with substantial frequencies and SHM, and heterologous boosting elicited increased SHM, affinity, and neutralization activity toward bnAb development. The results establish clinical proof of concept that heterologous boosting can advance bnAb-precursor maturation and demonstrate bAb priming in Africa where the HIV burden is highest.

FUNDING This work was supported by funding from the Gates Foundation Collaboration for AIDS Vaccine Discovery (CAVIMC INV-007368, CAVIMC INV-036842, CCVIMC INV-007371, VISC INV-008017, INV-032929, CAVD network INV-002916, VxPDC INV-008352, INV-007375, NAC INV-007522, INV-008813, INV-007385 and INV-005175); IAVI (IAVI 167627819, |AVI A0803); the lAVI Neutralizing Antibody Center; the National Institute of Allergy and Infectious Diseases (NIAID) (UM1 Al100663 and UM1 Al144462); the Intramural Research Program of NIAID; the Swedish Research Council (2017-00968); Moderna, Inc.; the U.S. Agency for International Development (USAID) (AID-5 OAA-A-16-00032 and IAVI A11257); and the U.S. President's Emergency Plan for AIDS Relief.

Two phase 1 clinical trials tested the first steps of a germline-targeting HIV vaccine strategy to induce broadly neutralizing antibodies (bnAbs). A nanoparticle antigen (multicolored sphere) delivered via mRNA activated immune cells, including bnAb precursor B cells (red) and other B cells (blue). The red cells began producing bnAb precursors (red Y-shapes). In one trial (IAVI G002), a second, distinct nanoparticle antigen (larger multicolored sphere) further guided those precursors toward developing the features of bnAbs. Credit: Scripps Research

Fig. S1. Conceptual schematic for germline-targeting vaccine design.

The study has been published in Science. YES, it is PEER-REVIEWED.
• www.science.org/doi/10.1126/...
• www.scripps.edu/news-and-eve...

I Know What You Did Last Supper

Are these designated cycle paths? Thinking about taking the family.

Moderna's combo Covid and flu mRNA shot outperforms current vaccines in large trial A combination shot would make it easier for people to get vaccinated against Covid and the flu at the same time.

Nobel Prize winning science.

Four Oxford researchers elected to the US National Academy of Last week, the National Academy of Sciences in the US announced the election of 120 members and 30 international members in recognition of their distinguished and continuing achievements in original

Congratulations to

- Prof Véronique Gouverneur
- Prof Desmond King
- Prof Andrew McMichael
- Prof Gero Miesenböck

Read more ⬇️

As a first #ImmunoSky post, I’m excited to share our latest pre-print, by the fantastic Mitchell Zheng!

www.biorxiv.org/content/10.1...

Our group is interested in virus-specific CD4 T cells, which means we often use in vitro stimulations (like the AIM assay) to find our cells of interest. 🧵 1/9

Happy to report that Mitchell's paper on CD4 T cells and AIM assays is now available online at Science Advances!

www.science.org/doi/10.1126/...

Thanks to all our collaborators in @viralvaxlab.bsky.social and @thedohertyinst.bsky.social, including @assocprofashhaque.bsky.social lab

CXCR4 down-regulation deconvolutes TCR-independent activation in the AIM assay
www.science.org/doi/10.1126/...

OMIP-114: A 36-Color Spectral Flow Cytometry Panel for Detailed Analysis of T Cell Activation and Regulation in Small Human Blood Volumes
onlinelibrary.wiley.com/doi/full/10....

In today's episode of the Night Science Podcast we talk with Martin Schwartz from Yale about the importance of stupidity in science: while learning science makes you feel smart, true scientific discovery often involves feeling stupid, because it means venturing into the unknown.

@arseblog.com a very…

Semiconductor quantum dots: Technological progress and future challenges A review highlights advances in the synthesis of colloidal quantum dots that have enabled numerous applications.

Quantum dots feature widely tunable and distinctive optical, electrical, chemical, and physical properties. They span energy harvesting, illumination, displays, cameras, and more.

Read more on #WorldQuantumDay: scim.ag/4j6gJOl

The diversity of CD8+ T cell dysfunction in cancer and viral infection - Nature Reviews Immunology Beyond exhaustion, CD8+ T cells can adopt various dysfunctional states, including tolerant, anergic, senescent, ignorant and dying states, that compromise their ability to eradicate viruses or tumours...

Our @natrevimmunol.bsky.social review with @abhishekgarglab.bsky.social, @deadoc80.bsky.social and Kellie Smith is out!

We attempt to integrate the data on CD8 T cell dysfunction into a new framework of hypofunctionality in cancer and chronic infections!

www.nature.com/articles/s41...

Can’t they just make Randy Pandas?

Spheromers reveal robust T cell responses to the Pfizer/BioNTech vaccine and attenuated peripheral CD8+ T cell responses post SARS-CoV-2 infection Our understanding of T cell responses in COVID-19 and vaccination is incomplete. Gao et al. examine SARS-CoV-2-specific T cell responses to infection and vaccination, revealing disparate kinetics betw...

Robust T cell responses to Pfizer/BioNTech vaccine compared to infection and evidence of attenuated peripheral CD8+ T cell responses due to COVID-19

Sounds ominous, but is it? Good vaccine responses but less viral responses..... or is context needed?

(X, Mar 20, 2023)

www.cell.com/immunity/ful...

I am super happy to share our latest article entitled "Decoding the molecular interplay of CD20 and therapeutic antibodies with fast volumetric nanoscopy", now published in Science www.science.org/doi/10.1126/...
It was my postdoctoral work mit @sauer-lab.bsky.social Congrats to all the co-authors!

THIS IS HUGE! Researchers at Scripps Research Institute have developed a groundbreaking UNIVERSAL coronavirus vaccine that triggers STONG immune responses AND shows promise in neutralizing MULTIPLE coronaviruses, including those responsible for causing COVID-19, MERS, AND even the “common cold.” 🧪🧵⬇️