Aaron Storey

Isotopically labeled internal standard peptides are unreasonably effective at establishing analytical measuring ranges for peptides liberated from tryptic digests. It’s unfortunate that IS panels are so expensive, otherwise more scientists would use internal standards to ground their measurements.

YouTube video by AFFINISEP Affinisep webinar "Implementation of a mass spectrometry typing assay in clinical proteomics"

Thanks Affinisep for the invitation to talk about our work. And thanks for making great desalting plates.
m.youtube.com/watch?v=v8t-...

Did you miss #asms2025 like me and wish you could ask a press correspondent how it was? Well look no further, #THEProteomicsShow with me and @proteomicsnews.bsky.social does just this! Find it wherever you find fine podcasts.

Science is more than a body of knowledge. It is a way of thinking; a way of skeptically interrogating the universe with a fine understanding of human fallibility. If we are not able to ask skeptical questions, to interrogate those who tell us that something is true, to be skeptical of those in authority, then, we are up for grabs for the next charlatan (political or religious) who comes rambling along. Carl Sagan

Dr. Anthony Fauci urges Americans not to accept "Normalization of Untruths".

That kind of skeptical, questioning, "don't accept what authority tells you" attitude of science - is also nearly identical to the attitude of mind necessary for a functioning democracy. They have very consonant values and approaches, and I don't think you can have one without the other.

A transformer model for de novo sequencing of data-independent acquisition mass spectrometry data A core computational challenge in the analysis of mass spectrometry data is the de novo sequencing problem, in which the generating amino acid sequence is inferred directly from an observed fragmentat...

A transformer model for de novo sequencing of data-independent acquisition mass spectrometry data
www.biorxiv.org/content/10.1...

The Need for Better Validation: Evaluating Aptamer and Proximity Extension Assays for Large-Scale Clinical Proteomics Studies - PubMed The Need for Better Validation: Evaluating Aptamer and Proximity Extension Assays for Large-Scale Clinical Proteomics Studies

Andy Hoofnagle and I wrote a comment about the paper also in Clin Chem pubmed.ncbi.nlm.nih.gov/40272410/

Correlations Within and Between Highly Multiplexed Proteomic Assays of Human Plasma - PubMed Precision of these 2 proteomics platforms in human plasma has diverged as the coverage has increased. These results highlight the need for careful consideration in platform selection based on specific...

This paper from Rooney et al is definitely worth a read if you are considering experiments with either Somascan or Olink. pubmed.ncbi.nlm.nih.gov/40172053/

Carl Sagan when asked, "Are you a Socialist?"

Panel 1: character crying at a desk Text: this IS hard Panel 2: character is now drawing determinedly Text: but I can do it

Little gentle reminder to all the human creatives out there 💪

On the cover: The blood-brain barrier is a defining feature of brain tumors, shaping their biology and therapeutic response. In this issue, Bejarano et al. use single-cell RNA sequencing and spatial analysis to construct a vascular atlas of primary and metastatic brain tumors, revealing distinct alterations in endothelial and mural cells. Glioblastomas undergo substantial vascular remodeling compared to low-grade gliomas, while brain metastases exhibit even greater permeability and increased immune cell interactions. This unveiling of vascular heterogeneity is depicted as a red pencil coloring in the intricate details of tumor-associated blood vessels within a map of the human brain wherein a tumor is surrounded by diverse immune cells. Image by Joana C. Carvalho.

Excited to see our image on the cover of #Immunity today 🤩

Congrats to the amazing @lbejarano.bsky.social & all authors for our #brain #tumor #vasculature study

www.cell.com/immunity/ful...

Huge 🙏 🙏 to the v talented @joanagcc.bsky.social for creating this super cover 🧠🧪

@cp-immunity.bsky.social

Tissue-like multicellular development triggered by mechanical compression in archaea The advent of clonal multicellularity is a critical evolutionary milestone, seen often in eukaryotes, rarely in bacteria, and only once in archaea. We show that uniaxial compression induces clonal mul...

That's dramatic (and cool): "archaea species can also form multicellular tissue–like structures when compressive forces are applied... ... These results establish multicellularity as a feature of all three domains of life" www.science.org/doi/10.1126/...

From the article: “Laboratory testing for this antigen [NDNF] by immunostaining or mass spectrometry can help identify cases of MN that are secondary to syphilis, prompting treatment with penicillin, which has shown to be effective in patients with both primary and secondary syphilis.”

Spectrum of Kidney Pathology in Patients with Syphilis Syphilis has been associated with multiple kidney diseases, primarily studied within small series. A large cohort of patients with active syphilis who underwent kidney biopsy were examined to evaluate...

Spectrum of Kidney Pathology in Patients with Syphilis - Kidney International Reports www.kireports.org/article/S246...

Arkana laboratories is seeking a highly motivated and detail-oriented Mass Spectrometry Lab Technician to join our newly established laboratory team. Please share and learn more in the link below.

Senior Scientist, Translational Proteomics, Global Mass Spectrometry Senior Scientist, Translational Proteomics, Mass Spectrometry Introduction to role Do you have a passion for Translational Proteomics and Mass Spectrometry? We are looking for a bold scientist to impa...

Our Translational Proteomics team is recruiting a Senior Scientist!
We're based in Gaithersburg, MD, USA.
A great opportunity to apply your skills to clinical trial samples, helping to develop new oncology drugs.
Please share!
astrazeneca.wd3.myworkdayjobs.com/Careers/job/...

This has only been possible through NIH funding.

Funding biomedical science through the NIH has been one of the greatest accomplishments of the United States of America for America, and for the world.

We are ALL better for the work that passionate scientists funded by the NIH have been able to do.

It pays to keep an open mind, but not so open your brains fall out.

It pays to keep an open mind, but not so open your brains fall out.

Ordered a cathode ray tube to demonstrate the principle of mass spectrometry. But wouldn’t you know it, I’m struggling to get an ion beam on the new instrument. Any ideas on getting a sufficient voltage across the terminals?

Francis Collins, the NIH Director for 12 years, led the Human Genome Project and other NIH efforts for 32 years, resigned today. Key words from his resignation letter
www.nytimes.com/2025/03/01/u...

NIH ban on renewing senior scientists adds to assaults on its in-house research Policy follows firings of tenure-track scientists and suspension of training programs

Of the 4000 researchers at NIH, only 20% are tenured. They’re going to fire all the rest as their contracts come up for renewal, absolutely decimating research at the NIH. This is catastrophic, not just for Americans, but for the whole world.

www.science.org/content/arti...

Was very proud of the US HUPO leadership who held an impromptu "Town Hall" during the US HUPO Conference this week to discuss the recent uncertainties and challenges in science. There were lots of anxieties, difficult questions, but sincere conversations. Most conversations were not centered 1/2

Did they have it?

National Protein day Our bodies crave protein. Here's a look at how one of the body's most basic building blocks works.

Happy National Protein Day! Cheers to my favorite cellular heteropolymer.

nationaltoday.com/national-pro...

Extraordinary claims require extraordinary evidence.

Extraordinary claims require extraordinary evidence.

A POGase is a huge advance in the glycomics field, which has typically relied on chemical or incomplete enzymatic releases (no Sia).
Expect a lot of multi-omic studies enabled by this (N-glycomics, O-glycomics, and proteomics) since the peptide backbone is left intact.
www.nature.com/articles/s41...

I had a wonderful time at my first #USHUPO conference. Great posters and great talks by great people who are driving the field to a better understanding of disease. Y’all are awesome. Thanks to Affinisep for the opportunity to talk about our work, and thanks to all for sharing their insights.

Sad but true.