Haselbach Lab

🚀 Today we're launching the 'Night Science Institute', a non-for-profit organization to lead a cultural shift in science! You may say we're dreamers 😉, but we think we're not the only ones. Perhaps today you will join us to make the Day Science and Night Science parts of the process live as one!

Thrilled to announce the nexy Austrian Cryo-EM Symposium, taking place 11th-12th November at @istaresearch.bsky.social, with speakers inc. @grigoriefflab.bsky.social, @oliveiramann.bsky.social, @bjgreber.bsky.social @svenklumpe.bsky.social @sebastianglatt.bsky.social ‪‬https://tinyurl.com/cryo2025

Great Day for science in Heidelberg - really sad for the VBC we will certainly miss you here.

AlphaFold predictions are valuable hypotheses and accelerate but do not replace experimental structure determination - Nature Methods An analysis of AlphaFold protein structure predictions shows that while in many cases the predictions are highly accurate, there are also many instances where the predicted structures or parts of pred...

Great work highlighting how important it is to carry out experimental structure determination instead relying on AlphaFold predictions. #crystallography #structure #biology #chemistry

www.nature.com/articles/s41...

Indeed it was a rather eventful meeting with a lot of great science

We are happy to release LocScale2.0: a tool for context-aware, confidence-weighted cryoEM map optimisation.

Taking two half maps as input, LcoScale-2.0 produces feature-enhanced maps along with a robust confidence score that guides objective map interpretation.

cryotud.github.io/locscale/

The 'RNA meets Protein Decay' @embo.org workshop was really a blast. Thanks to everyone for the lively discussions!
I am especially honoured that my poster was awarded with one of the two poster prizes 😇

It was amazing to do a Night Science workshop today at the Karolinska Institute in Stockholm!

@science.org 🧫🧬❄️🔬 Molecular basis of influenza ribonucleoprotein complex assembly and processive RNA synthesis | Science www.science.org/doi/10.1126/...
@yiweichang.bsky.social www.yiweichanglab.org @jiwasa.bsky.social #virology #Influenza #Cryo-EM #StructuralBiology #RNA #polymerase

Congrats to @lgrundmann.bsky.social for winning a poster award at #EMBORNAmeetsproteindecay

If u ever had to depict proteins as schematics in papers, u know the dilemma: how to create a pretty “blob”?
If structure ends up big & central, u’ll render it with PyMol et al, but for a truly schematic depiction u want a biorender-style icon, but for YOUR protein.
Think I’ve finally found a way.

Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social,
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social

🔗 Preprint here tinyurl.com/3a74uanv

I started on this around 2006 so only taken 20 years! The project itself started over 30 years ago. Great to finally see the elusive structure.

Thank you Khaled :)

Thanks a lot. As ubiquitin and the proteasome are well conserved across all eukaryotes I believe it will look very similar in plants :)

Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...

Structural basis of K11/K48-branched ubiquitin chain recognition by the human 26S proteasome Beyond the canonical K48-linked homotypic polyubiquitination for proteasome-targeted proteolysis, K11/K48-branched ubiquitin (Ub) chains are involved in fast-tracking protein turnover during cell cycl...

similar results have been obtained earlier this year by @pdracakowski.bsky.social using a different kind of K11/K48 branched chain. See also their preprint: www.biorxiv.org/content/10.1... 9/9

This was a great team effort and we thank our Funders: @boehringerglobal.bsky.social, @fwf-at.bsky.social @wwtf.at @erc.europa.eu, the NIH. We also thank for the great support of the @impvienna.bsky.social and the help of the great facilities at VBCF 8/9

Finally, we tested the interfaces genetically with our collaborators in the @johanneszuber.bsky.social lab 7/9

The K11 branch uses a new binding interface between RPN2 and RPN10, further ubiquitins of this branch are pointing towards the solution and may not further interact with the proteasome 6/9

With our collaborator Nick Brown from UNC we were able to generate K11/K48 branched ubiquitin chains that are the chains predominantly used for cell cycle control. 5/9

The 4th ubiquitin is thereby having the most interactions explaining why it is this one that boosts the degradation at least in vitro. 4/9

This enabled us to solve the structure of a ubiquitin chain bound proteasome with cryoEM. All four ubiquitins are clearly visible. Unexpectedly, we see the ubiquitin chain wrap around the UIM1 motif of the ubiquitin receptor RPN10 in a spiral like fashion. 3/9

Our collaborators @klanglab.bsky.social generated homogeneous tetraubiquitin chains with K48 linkage that could not be cleaved by the proteasome. 2/9

Structural basis for the ubiquitin chain recognition of the human 26S proteasome Proteasomal degradation is a fundamental process for all eukaryotic life. A protein destined for degradation is first tagged with a polyubiquitin chain, which is selected by the proteasome. Different ...

New Preprint: We have solved the structure of the proteasome in complex with two different ubiquitin chains: www.biorxiv.org/content/10.1... This work is spearheaded by PhD Student Sascha Amann
Let me walk you through it 🧵 1/9

Amazing! Congratulations!

🚨 Hiring Alert – Senior Scientist (Biology) 🚨
My lab is looking for a senior scientist to lead functional genomics efforts and decode how small molecules reshape biology
🧬 Lead screens
🤝 Collaborate with top-tier AI/chemistry/biology minds

Interested? then apply: cemm.onlyfy.jobs/job/t7lhh1kz

We are looking for PhD students using time resolved cryoEM or cryoET on topics of the ubiquitin proteasome system, fertility and immunity. However, there is freedom to shape projects in own ways.

my bad - then I agree

Okay really nice video, but except that the instrument is now black it does not give any detail on the Krios5.