Isabelle Busseau

The ORB2 RNA-binding protein represses translation of its target transcripts during the Drosophila maternal-to-zygotic transition via its functionally conserved Zinc binding 'ZZ' domain https://www.biorxiv.org/content/10.1101/2025.07.10.664187v1

Direct observation of translational activation by a ribonucleoprotein granule - Nature Cell Biology Chen et al. perform single-molecule imaging of translation at ribonucleoprotein (RNP) granules. They show that RNP granule surfaces are sites of nanos mRNA translation, whereas the granule interior is...

Chen, R., Stainier, W., Dufourt, J., Lagha, M., Lehmann, R. (2024). Direct observation of translational activation by a ribonucleoprotein granule. Nat Cell Biol, 26(8):1322-1335 www.nature.com/articles/s41...

Why a database of bug genes could be one of Trump’s most devastating cuts at Harvard FlyBase, the world's only fruit fly database, is in the crosshairs of Trump's attack on Harvard. Here's why it could matter for your health.

MassLive.com recently interviewed several Harvard FlyBase people and wrote this news article about the importance of FlyBase: tinyurl.com/4chpts9f

Images from the linked preprint showing Wingless stainings in the Drosophila wing imaginal disc. Targeting vps15 with two indepependent RNAi lines (paper also shows the same with a third line) does not result in a phenotype. In contrast, targeting vps15 with two independent sgRNA constructs and Cas9 results in strong accumulation of Wingless protein in perturbed cells.

Why use #CRISPR in #Drosophila when RNAi works so well? Because for some genes well is not good enough.

Check out new preprint from the lab demonstrating a role for Vps15 in Wingless trafficking. Something that was missed in several RNAi screens.

www.biorxiv.org/content/10.1...

Tired of having to explain this again and again, I made an info- graphic of #Drosophila stock keeping. Any comments? @bdsc.bsky.social @flybase.bsky.social

"We should include eugenics in our undergraduate classes, reminding students that scientists must speak out when others lie about science and use it to further their political views," says professor @peiferlabunc.bsky.social.

www.cell.com/trends/genet...

@cp-trendsgenetics.bsky.social

I am pleased to announce the upcoming thematic school on small regulatory RNAs. It will take place in Sète from October 6–10, 2025. Pre-registration is open until June 6. For further information, please refer to the attached flyer and visit: internat25.sciencesconf.org. @cnrsbiologie.bsky.social

Play this Crossword - Each answer is the full name of a fly gene Each answer is the full name of a fly gene

FlyBase made a fly gene themed crossword puzzle for #Dros25. If you missed getting one (we have lots at the FlyBase booth), or you couldn’t be here, it’s also available online. puzzleme.amuselabs.com/pmm/crosswor...

#StandUpForScience a rassemblé près de 15 000 personnes partout en France. Des scientifiques, équipes, laboratoires, ou des associations citoyennes ont affiché leur soutien par des photographies prises sur leur lieu de réunion ou de travail. Nous les avons assemblées 👇
standupforscience.fr/photos/

A photo of a young Lilian Vaughan Morgan, sitting with a book on her lab staring into the distance.

A photo of an older Lilian Vaughan Morgan, sitting at a lab bench with a microscope and dozens of glass milk bottles bunged with cotton. Lilian is in the middle of applying ether to anaesthetize a bottle of flies.

This #InternationalWomensDay I want to tell the story of Lilian Vaughan Morgan. I learned of Lilian this past year - what a badass.

Lilian was a leading scientist when women weren't welcome in the lab, nor even the department. But she contributed immensely to the genetics of sex determination

1/n

💪🇺🇸🇫🇷

A guide to the biogenesis and functions of endogenous small non-coding RNAs in animals - Nature Reviews Molecular Cell Biology Many new small RNA species and their derived fragments have been recently identified in animals. This Review discusses the biogenesis and functions of both structural small RNAs, such as tRNAs and sma...

Our review on small endogenous RNAs in animals is online: www.nature.com/articles/s41.... We thank @martinjsimard.bsky.social, @svobodalab.bsky.social and the third, anonymous, reviewer for helping us improve our manuscript. @lbmcinlyon.bsky.social @cnrsbiologie.bsky.social

Wikipedia, trop libre pour le libertarien Musk.

J'ai donné, et je vais passer au don mensuel.

Il dépend de chacun de sauver Wikipedia.

Calling #Drosophila #grad students #postdoc and #pretenure #faculty to participate in FlyCROSS and be matched by a #mentor of your preferences to gain guidance to your unique needs !
#genetics #dros25 #phdlife

@flybase.bsky.social @bdsc.bsky.social

We are thrilled to announce that IRL JEDI meetings will restart in 2025.
You can already register at flyjedi.sciencesconf.org where you'll find a preliminary program.
Looking forward to meeting you and restart this network of fly people!

Come with me as I troubleshoot this very high GC PCR toward clonable homology arms in an actinobacteria. Testing DMSO, Betaine 1M, and Propylene Glycol to see if we get amplification. Arms are 1000bp. Full length with arms and gene is 2800bp. Let's go!

🧵 1/n

Fresh off the press, our work on wing deployment in Drosophila 🪰:
www.nature.com/articles/s41...
Work by: Simon Hadjaje, Ignacio Andrade-Silva, Marie-Julie Dalbe and Raphaël Clément

"We report that numerous “miRNAs” previously reported in EMT and cancer contexts are not incorporated into RISC and are not capable of endogenously silencing target genes, despite the fact that hundreds of publications in the cancer field describe their roles"

www.biorxiv.org/content/10.1...

Our new preprint is out - please share!

We asked: How can we make CRISPR knockouts in vivo more efficient? And set up an assay that enables detection of CRISPR cutting with 100s-1000s of sgRNAs over entire chromosome arms in living animals.

Grab a ☕ and let’s dive in. 🧵🧪 1/n
#CRISPR #geneediting

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Bottom of e and top right of g

Exciting highlights of the recent complex life of #RNA symposium @embl.org featuring Matt Disney, Juan Valcarcel, @lapassmore.bsky.social, Herve Le Hir, Andreas Meyer, Yiliang Ding, Seitz Herve, Maria Hondele, @juliusbrennecke.bsky.social, Bahareh Torkzaban

www.embl.org/news/science...

Happy FlyDay (11/18 → w1118)! 🎉

Let's take a moment to thank (and follow!) our colleagues at
@flybase.bsky.social ky.social and @bdsc.bsky.social y.social for their invaluable contributions to the Drosophila research community.

De toute façon ce sont deux choses différentes. Les labos ne sont pas forcément dans la même section que les chercheurs et inversement. Ça n’a pas d’incidence particulière. L’important est que la section soit capable d’évaluer votre travail.

Coucou, il y a du monde ici?
Je crois que je vais être de plus en plus actif sur cette appli où le ciel est bleu. On va parler astronomie, sciences et apprendre en s'émerveillant!

N'hésitez pas à partager pour recréer une belle communauté ici!!

D’aillleurs j’en profite pour vous rappeler que ce choix de section lors des élections est le moyen le plus facile pour changer de section puisque vous serez automatiquement rattaché à la section dans laquelle vous avez voté #CoNRS

Screenshot of Table 1 from https://doi.org/10.1002/dvg.23600. Shows off-target expression for 11/12 Gal4 driver lines tested. But shoutout to Ptth-Gal4 for being a real one.

I hope this paper gets the attention it deserves in the #Drosophila community! Many common "tissue-specific" Gal4 driver lines may have more off-target expression than originally reported. Behold this table and weep for your favorite Gal4. doi.org/10.1002/dvg....