Kashish Singh

Our latest work, in collaboration with the terrific @kashishsingh.bsky.social in the Carter lab @mrclmb.bsky.social is out. So proud of this study and everyone involved...

In-cell structure of a LINC complex reveals the molecular basis for membrane remodelling and head-to-tail coupling in sperm cells In eukaryotic cells, LINC complexes physically bridge the two nuclear membranes to cytoskeletal filaments, transmitting mechanical forces to the nucleus. Due to their dynamic and membrane-embedded nat...

Fantastic work by the group of @matteoall.bsky.social : a SUN5:Nesprin3 complex at the head:tail junction of sperm cells. 🤯 #teamtomo

www.biorxiv.org/content/10.1...

Check out our latest work now out on biorxiv www.biorxiv.org/content/10.1...

Check out this exciting new study from the @rgassmann.bsky.social lab that we are thrilled to be part of — revealing that CDR2 is a dynein adaptor that regulates ER sheet organization!!

Now hiring: Research Group Leader Structural Studies Division To develop artificial intelligence/machine-learning methods to further our understanding of biology at molecular and/or cellular scales Apply by 18th March

Join our Structural Studies Division, leading a research group developing computational methods to address questions in molecular & cellular biology!
You will be supported by core funding, excellent facilities & enthusiastic colleagues.
Apply by 18 MAR
www.nature.com/naturecareer...

Today in #journalclub, we looked at this fantastic new method from the @doudna-lab.bsky.social in Nature Biotechnology! They show how CRISPR–Csm enables single-molecule live-cell imaging of endogenous RNAs. Super cool!
www.nature.com/articles/s41...

Senior Administrator (Divisional Administrator) - Structural Studies - LMB 2565 at MRC Laboratory of Molecular Biology Start your UK & international job search for academic jobs, research jobs, science jobs and managerial jobs in leading universities and top...

We are looking for a senior administrator to take care of the Structural Studies Division at @mrclmb.bsky.social. Come and work for the benefit of human health in a fantastic environment. Happy to chat!

www.jobs.ac.uk/job/DLY086/s...

PITHD1: An Endogenous Inhibitor of the 26S Proteasome During Cellular Dormancy Cellular dormancy represents a state of regulated growth arrest essential for diverse biological processes, from reproduction to cancer progression. While mechanisms controlling protein synthesis in d...

The second of our two new preprints addresses the question of how proteasomes can be kept dormant for instance in oocytes. This study was spearheaded by first author Sascha Amann. biorxiv.org/cgi/content/... (1/6)🧵

A cryo-electron tomography study of ciliary rootlet organization pubmed.ncbi.nlm.nih.gov/39641991/

This scheme shows the cargo adaptor FHF in the middle with arrows pointing to distinct microtubule and actin-based motors (dynein-dynactin, KIFC1, KIF1C and myosins V, IX and X as well as Tropomyosin 1). It also shows a link to Rab5 marked early endosomes. The hypothesis is that FHF coordinates multi motor binding at the early stages of endocytosis. The PhD project would address the hierarchy of motor binding and how these motors coordinate to allow the newly formed vesicle to transition from the actin to MT cytoskeletal network.

If you’re interested in how molecular motors coordinate to power intracellular trafficking events (e.g. endocytosis), I’ve got a PhD position available to study this at vibrant Bristol uni. In this project, we will capture highly dynamic events by #cryoEM, single molecule imaging & cell biology. 🔬❄️🧪

A multivalent adaptor mechanism drives the nuclear import of proteasomes Nuclear protein homeostasis, including the turnover of transcription factors, critically depends on nuclear proteasomes. After each cell division, proteasomes need to be re-imported into the newly for...

We are very excited to share our recent preprint on the nuclear import mechanism of the proteasome, driven by the multivalent adaptor AKIRIN2! www.biorxiv.org/content/10.1... 🧵 1/6

The intraflagellar transport cycle - Nature Reviews Molecular Cell Biology Intraflagellar transport (IFT) ensures delivery of selected proteins into cilia. The IFT protein complexes IFT-A and IFT-B polymerize at the base of the cilium to form an anterograde train that facili...

Our review of Intraflagellar transport is out now! We cover lots of the recent advances in our understanding of IFT, and where we think there are still some gaps.
www.nature.com/articles/s41...

We have three PhD projects with motor-cytoskeleton theme though collaborations with three new(ish) PIs at Bristol Biochemistry supported by SWBio_DTP with Berni Carroll (lysosome transport), @abidalilab.bsky.social (endocytosis) and Jessica Cross (protein design)
see swbio.ac.uk/biomolecular...