Wieczorek Lab

Cryo-EM resolves the structure of the archaeal dsDNA virus HFTV1 from head to tail This structure of an archaeal tailed virus (arTV) provides detailed insights into arTV assembly and infection mechanisms.

Out in Science Advances: Our #cryoEM structure of HFTV1, a virus infecting the halophile #archaea. *First full atomic structure (containing all structural proteins) of any tailed virus!* Congrats and thanks to all co-authors and our fantastic collaborators! www.science.org/doi/10.1126/...

Introducing the first 3DEM Model-Map percentile slider to the wwPDB validation report Introducing the latest wwPDB Percentile slider based on the Q-score model-map validation metric

Today, @rcsbpdb.bsky.social introduces Q-score in EM validation reports. Q-score assesses model-map fit. A new percentile slider will compare an entry’s average Q-score to the full archive and a resolution-matched subset. This would help judge whether the fit is typical for the reported resolution.

Huge congratulations to Girish and team on this beautiful study!

Paper alert! 📢 How do cells strike the perfect balance during meiosis? ⚖️ A new study from the Matos lab in @natureportfolio.nature.com uncovers how Holliday junctions and the synaptonemal complex safeguard crossovers ➡️ tinyurl.com/2k4mmpzu

@univie.ac.at
@meduniwien.ac.at

Feeling excited & grateful 🌟

Honored to receive the Falling Walls Science Breakthrough 2025 (Life Sciences) — for our team’s live ovulation imaging.

Thanks to Tabea Lilian Marx, Christopher Thomas & our lab!
www.mpinat.mpg.de/5118437/pr_2...
#FallingWalls #ScienceSummit25 #ScienceBreakthroughs

Exciting new study from new-PI Mohinder Pal on the role of CCDC103 and it's links to PCD as the most recent dynein assembly factor (DNAAF19). Congratulations to the entire team.

@Mohinder Pal (currently not on Bluesky)
@mike-wieczorek.bsky.social
@laurencepearl.bsky.social

CCDC103-mediated assembly of the R2C complex links RUVBL1-RUVBL2 to Primary Ciliary Dyskinesia Primary ciliary dyskinesia (PCD) is a genetic disorder caused by defective cilia motility, powered by axonemal dynein motors. Assembly of these motors is facilitated by the molecular chaperone HSP90, ...

New pre-print on the role of CCDC103/DNAAF19 in dynein assembly highlighting it's link to PCD.

www.biorxiv.org/content/10.1...

CCDC103-mediated assembly of the R2C complex links RUVBL1-RUVBL2 to Primary Ciliary Dyskinesia https://www.biorxiv.org/content/10.1101/2025.09.11.675549v1

Asgard archaea were first found in mud at the bottom of the North Sea. Today, cellular biologists Martin Pilhofer and Christa Schleper are exploring one of their more elusive structural elements: a totally tubular system that looks a lot like our own.
www.quantamagazine.org/tiny-tubes-r...

Patrick's paper is finally out, a label-free method to measure the composition of multicomponent biomolecular condensates!

Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators.

www.nature.com/articles/s41...

Excited to see this work published online at @cp-cell.bsky.social today!

www.cell.com/cell/fulltex...

🚨
New Preprint!
Ex-dSTORM resolves fine details of the molecular architecture of CCPs, the 8-nm periodicity of microtubules, and the docking site of synaptic vesicles at the presynapse of hippocampal neurons.

▶️ www.biorxiv.org/content/10.1...

Cells can form patterns within themselves just like embryos do. How? Connie Yan's new preprint shows how the anterior-posterior cytoskeleton pattern in Stentor is dictated by regionalized scaffolding proteins

www.biorxiv.org/content/10.1...

Check out 🧐 our new preprint 📜:
www.biorxiv.org/content/10.1...
Congrats🎉 to @vasilgaisin.bsky.social and Corina Hadjicharalambous, who studied contractile injection systems directly in hot spring♨️ bacterial mats🦠
#microsky #teamtomo #Chloroflexota

Check out our latest work now out on biorxiv www.biorxiv.org/content/10.1...

Cryo-EM structures of Egl-BicD-RNA complexes reveal how diverse mRNAs are selected for subcellular localization www.biorxiv.org/content/10.1101/2025.08.02.668268v1 #cryoEM

YouTube video by MRC Laboratory of Molecular Biology Structure Prediction and Design using AlphaFold – Sami Chaaban

Great talk by @sami-c.bsky.social about the proper use and caveats of AlphaFold2/3 predictions, highly recommended to watch www.youtube.com/watch?v=z7vy...

Exciting Aug 1️⃣ for two incredible people- Gregor Weiss and Jingwei Xu! Both are starting their independent labs 🔬🧪 TODAY 🥳
I am incredibly thankful 🙏 for their contributions to the lab and that I could be part of their journey
-Martin
@xujwet.bsky.social
@weiss-lab.bsky.social
It was a wild ride👇

A conserved mechanism of membrane fusion in nuclear pore complex assembly https://www.biorxiv.org/content/10.1101/2025.07.21.665908v1

Happy to share our latest work on the structure and assembly of holocentric kinetochores! Huge thanks to Ines for a very fruitful collaboration, Claudio for all the support, and congratulations to Christine and all co-authors!

www.biorxiv.org/content/10.1...

Paumier, Sauvanet, Gigant @mikicalab.bsky.social highlight work from Muñoz-Hernández, Xu et al @mike-wieczorek.bsky.social (rupress.org/jcb/article/...) which describes new insight into the means by which NEDD1 protein recruits the γ-TuRC to microtubule-organizing centers rupress.org/jcb/article/...

Thanks to the Gigant lab for writing this insightful spotlight that discusses our recent work on how NEDD1 binds to and might recruit the γ-TuRC to MTOCs:

Figure showing microtubule-nucleating activity of CDK5RAP2 scaffolds with γ-TuRCs.

Manolo Rios, Jeffrey Woodruff and colleagues use purified human proteins to define the minimal modules for centrosome scaffold assembly and microtubule aster nucleation.

journals.biologists.com/jcs/article/...

Excited and honored to be joining the EMBO Member community 🥳 Congratulations also to all the other newly elected members! -Martin

After 5 amazing years @pilhoferlab.bsky.social I'm starting my lab @embl.org Grenoble this November embl.org/wollweber
We'll image Asgard archaea and many other strange microbes ( #archaeasky, #protistsonsky..) to understand eukaryogenesis
First job ad: #teamtomo scientist!🔬❄️ tinyurl.com/2rdu2ze6

Spatiotemporal orchestration of mitosis by cyclin-dependent kinase - Nature Experiments in fission yeast show that cyclin-dependent kinase is first activated in the nucleus, from which the mitotic signal is propagated from CDK at the centrosome to the cytoplasm.

I'm very excited that my first postdoc paper is out now at @nature.com!
www.nature.com/articles/s41...

I developed new single cell CDK sensors to investigate how CDK orchestrates mitosis including investigating bistability/hysteresis, how activity is propagated, and the role of centrosomes.

🚨 New preprint!
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...

Cell state-specific cytoplasmic density controls spindle architecture and scaling Nature Cell Biology - Kletter et al. show that cell state-specific cytoplasmic density controls spindle architecture and scaling in neural differentiation, suggesting that the physical properties...

✨Thanks to amazing students @tobiaskletter.bsky.social, postdocs @biswashere.bsky.social & collaborators @vasilyzaburdaev.bsky.social‬ & ALMF @embl.org ✨

👉Cytoplasmic material properties control spindle architecture and scaling 👉 out today @natcellbio.nature.com rdcu.be/eqPr8

Muñoz-Hernández, Xu, et al. @mike-wieczorek.bsky.social @ethz.ch report #cryoEM structures revealing how NEDD1’s C terminus forms an α-helical tetramer that docks onto the γ-TuRC without inducing major conformational changes in the complex during its recruitment to MTOCs rupress.org/jcb/article/...