Rupert Faraway

Collective homeostasis of condensation-prone proteins via their mRNAs - Nature The authors discover a homeostatic process termed interstasis, in which an increased concentration of proteins within RNA–protein condensates induces the sequestration of their own mRNAs.

This is a fascinating paper that particular types of RNA binding proteins with IDRs that target nuclear speckles also can recruit their own RNAs to nuclear speckles as a negative feedback mechanism for condensation the authors call "interstasis" www.nature.com/articles/s41...

Molecular basis of polyadenylated RNA fate determination in the nucleus Eukaryotic genomes generate a plethora of polyadenylated (pA+) RNAs[1][1],[2][2], that are packaged into ribonucleoprotein particles (RNPs). To ensure faithful gene expression, functional pA+ RNPs, in...

How are RNAs sorted for export vs. degradation in the nucleus? In collaboration with @heick.bsky.social’s lab we (@clemensplaschka.bsky.social and @juliusbrennecke.bsky.social labs) discovered a direct mechanistic link between the export and decay machineries: www.biorxiv.org/content/10.1... (1/x)

Integrative profiling of condensation-prone RNAs during early development Complex RNA–protein networks play a pivotal role in the formation of many types of biomolecular condensates. How intrinsic RNA features contribute to condensate formation however remains unclear. Here...

I am thrilled to share the first manuscript from my group on identifying sequence and structural features promoting the condensation of RNAs that are organising highly interconnected RNA networks, thereby expanding our understanding of higher-order transcriptome assemblage.
shorturl.at/pmLIj #RNASky

I would rather avoid radioactivity, here.

Has anyone spiked in a fluorescent or clickable ATP into an in-vitro poly-adenylation reaction to label some RNA? Or anything similar (e.g. clickable nucleotide)? Preferably using E. coli PAP. I don't care if it acts as a chain terminator - that might actually be preferred.

A multivalent adaptor mechanism drives the nuclear import of proteasomes Nuclear protein homeostasis, including the turnover of transcription factors, critically depends on nuclear proteasomes. After each cell division, proteasomes need to be re-imported into the newly for...

We are very excited to share our recent preprint on the nuclear import mechanism of the proteasome, driven by the multivalent adaptor AKIRIN2! www.biorxiv.org/content/10.1... 🧵 1/6

1/10
Thrilled to share our latest research on 3′UTR-derived RNAs, now published in BMC Biology! doi.org/10.1186/s129...
Here’s what we found:

Can MS2 loops stall reverse transcription? Does anyone know? I'm using Superscript IV at 50C.

All the best ideas come from staring at IGV until your eyes rot.

Thanks! I went and tracked it down anyway. Cool paper!

For me, the link doesn't work. Just a heads up! Maybe it's specific on my end, but it looks like the URL got truncated.

Luckily for you, Bluesky is inactive enough that it can't take up much time.

Do we just have to write #RNAbiology in a message to access the feed?

Created an #RNAbiology feed to talk RNA science! Let's use it.

@onamy.bsky.social @mgblango.bsky.social @graveley.bsky.social @nickingolia.bsky.social @hogglab.bsky.social @quaidmorris.bsky.social @snf.bsky.social @lucksmith.bsky.social

I read every Arjun Raj tweet at least 10 times, so seeing it both on BlueSky and Twitter saves me time, if anything.

Why not bleeting?

I read that you're supposed to join feeds by posting some keyword or something? I dunno I'm boomer-pilled.

I'll be looking to hire another research associate (>$100k/year + benefits & retirement) shortly. Experience in molecular biology and pharmacology would be ideal, but not essential. Job ad will go up in a bit, but if anyone knows anyone looking I would be happy to chat with them.