Swaminathan Lab

The nuclear periphery confers repression on H3K9me2-marked genes and transposons to shape cell fate - Nature Cell Biology Marin et al. report the role of lamin proteins and the lamin B receptor (LBR) in chromatin positioning at the nuclear periphery. Knockout of all lamins and LBR in mouse embryonic stem cells leads to h...

Another paper bluetorial! Today: how does the spatial location of genes influence their function? (1/n) www.nature.com/articles/s41...

Amazing work Abby! Congrats to all authors

YouTube video by Science Communication Lab The Most Beautiful Experiment: Meselson and Stahl

Just learned that Frank Stahl (of the Meselson and Stahl DNA replication experiment ("the most beautiful experiment in biology") died at the beginning of April, to no fanfare. Here's a lovely video of them reminiscing: www.youtube.com/watch?v=7-tn...

Amazed Inspired GIF Alt: Amazed Inspired GIF

The feeling when you get back to the lab after a week at a gordon conference - totally inspired and energised after hearing incredible science and meeting amazing people. Thank you @tanentzapflab.bsky.social and @sarawickstrom.bsky.social for GRC Fibronectin, Integrins and related molecules.

as usual beautiful data. big congrats!

Fantastic surprise to have Reinhard Fässler visit Lund to talk about kindlins, integrins and mitosis.

Postdoc in Experimental Oncology - Lund (Stad), Skåne (SE) job with Lund University | 12838990 Lunds universitet, 009999 LUNDS UNIVERSITET, 310000 MED Lund University was founded in 1666 and is repeatedly ranked among the world’s top universi...

Join us for innovative explorations of the tumor microenvironment🔬 Learn cutting-edge technologies and grow in a dynamic environment💡 The postdoc will work alongside a doctoral student from @adaptmet.bsky.social, gaining access to collaborative partners across 🇪🇺.
Apply now👇
tinyurl.com/4mby45hf

Holy wow-- this is amazing!! Congratulations!

RNA neoantigen vaccines prime long-lived CD8+ T cells in pancreatic cancer - Nature In a phase 1 trial, patients with pancreatic ductal adenocarcinoma who were treated with surgery and bespoke neoantigen mRNA vaccines combined with anti-PD-L1 and chemotherapy exhibited marked lo...

Seems like a good time to share and appreciate this incredible
window to future cancer treatments and the opportunities ahead (and also appreciate its journey from test tubes to bacteria to cell culture to animal models to covid vaccine and now to this-)
www.nature.com/articles/s41...

Colorado CDC researcher terminated amid federal budget cuts while working on Lyme disease projects Colorado research biologist Joe Cardiello was excited about being involved in what could become lifesaving research for people who have been impacted by Lyme disease.

So many heartbreaking stories right now coming from the US. Joe was a post-doc fellow here at WCMM Lund University and moved back to Fort Collins for family and career.
www.cbsnews.com/colorado/new...

Dear federal scientists: If you have been affected by the probationary firings and would be willing to speak with Science, please reach out to me by email (klangin@aaas.org) or on Signal. We can guarantee annonymity.

Big first for our lab on Friday as Wesley became its first PhD graduate. Congrats Wesley and a big thanks to an amazing committee including @bengoult.bsky.social (opponent), @sciezgin.bsky.social , Gunilla Westergren-Thorsson, Catharina Hagerling and Daniel Engelbertsen.

what a fantastic lineup!!

🚨🚨🚨Come and join us at the Wallenberg Center for Molecular Medicine- tenure track positions in broad areas of 1)basic research and 2)advanced therapy in regenerative medicine.
🚨Deadline Feb 10th. DM me if you have any questions
lu.varbi.com/en/what:job/...
lu.varbi.com/en/what:job/...

Amazing and beautiful work as usual from @alushinlab.bsky.social

This work has led us now into a whole range of new projects and collaborations that we are very excited about. This project was a collaboration with the groups of Adam Simonsen at SDU, Cathy Merry and Amanda Wright at Nottingham and Pontus Nordenfelt

This work was inspired and stands on the shoulder of amazing work from several labs that have been developing alginate based hydrogel system and/or have been working on ECM stiffness-mechanotransduction pathways in epithelial structures for decades

Mechanistically, using antibodies, inhibitors etc, Kabilan found that this process of in-situ stiffening induced ECM remodelling and invasion of normal mammary acini was driven by beta1 integrin-FAK-Piezo1 pathway with Piezo1 specifically being downstream of integrin activation and signalling.

Kabilan then followed the process of in-situ stiffening induced changes in acini morphology and ECM properties over time and found interesting and "dynamic" changes in relative ECM enrichment which coincided with different stages of growth and the onset of invasion

Interestingly, he found several differences in basement membrane and ECM(Fibronectin) enrichment and organisation between acini embedded in in-situ stiffened gels compared to acini formed from single MCF10A cells embedded in stiff gels from Day 1 (see below)

Here Kabilan first embedded single MCF10A cells in a soft interpenetrating network hydrogel system and allowed it to form a normal mammary acini with its own native ECM environment. He then in-situ stiffened these gels thus mimicking tumour growth and invasion on normal 3D structures-

β1 Integrin–FAK–Piezo1 signalling axis drives in-situ stiffening mediated ECM remodelling and invasion of 3D breast epithelium Stiffening of tissue is a hallmark of cancer progression, driving invasive phenotypes through complex interactions between cells and their extracellular matrix (ECM). However, the mechanisms linking m...

I am very excited to (delayed) share our preprint which has been led by Kabilan Sakthivel on mechanisms by which ECM stiffening of normal 3D tissues in their native environment drives invasive phenotypes
www.biorxiv.org/content/10.1...

For early career researchers interested in the Fibronectin, Integrins and Related Molecules GRS/GRC this May, don’t forget to sign up! The deadline for submitting an abstract to be considered for an oral presentation for the GRS is less than a week away! Register here: www.grc.org/fibronectin-...

Mammary tumor tissue stained with collagen (green) and nuclei (red)

Are you working on #ECM or mechanobiology in the mammary gland or in breast cancer? Together with Jason Northey we are guest editing a collection on the topic for the Journal of Mammary gland biology and Neoplasia. Submission of articles is now open! link.springer.com/collections/...

This is a scientific life changing experience-

this is beautiful. Fantastic work.--

Courses Intent to Apply Form Cold Spring Harbor Laboratory Meetings & Courses -- a private, non-profit institution with research programs in cancer, neuroscience, plant biology, genomics, bioinformatics.

Hey PIs, do your trainees need to learn light microscopy and image analysis? Send them to us! Financial aid is available.
meetings.cshl.edu/coursesinten...

Genetic Analysis of a Sarcoma Accidentally Transplanted from a Patient to a Surgeon | NEJM Modern concepts of cancer immunology originated from the classic observations by Jensen, Loeb, Tyzzer, and Little in the early years of the 20th century of the rejection of transplanted allogeneic ...

Crazy!- A rare case of malignant tumor transplantation from patient to surgeon:
www.nejm.org/doi/full/10....

β1 Integrin–FAK–Piezo1 signalling axis drives in-situ stiffening mediated ECM remodelling and invasion of 3D breast epithelium Stiffening of tissue is a hallmark of cancer progression, driving invasive phenotypes through complex interactions between cells and their extracellular matrix (ECM). However, the mechanisms linking m...

Sakthivel K, et al. {beta}1 Integrin-FAK-Piezo1 signalling axis drives in-situ stiffening mediated ECM remodelling and invasion of 3D breast epithelium. bioRxiv posted 8 January 2025 doi:10.1101/2025.01.07.631639. www.biorxiv.org/content/10.1...

In all seriousness,use CiteAb and Antibodypedia, look for KO/KD validated antibodies, best - recombinant, second best - monoclonal, and if possible do your own KO/KD controls, or induction by ligand/small molecule,if not - compare hyperexpressing with low expressing cells (from depmap/protein atlas)