Bronwyn Lucas

Nominations for the June Almeida award for Mid/Senior-Career Women in Cryo-EM are now open! Submit your nomination via this link www.biophysics.org/awards-fundi... by November 14th to recognize an exceptional woman in our community.

Muyuan Chen has turned structural biology into an immersive experience with his new video game Meowtabolism, now available on Steam.
Try the demo here: store.steampowered.com/app/4045010/...
Give Muyuan feedback: steamcommunity.com/app/4045010
#ScienceGaming #StructuralBiology #CryoEM #STEMOutreach

It makes sense to me that this would work. Nice Oli

🚀 Check out our new preprint on extending the scope of single-particle cryo-EM with 2D template matching!
Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching is now available on bioRxiv!
www.biorxiv.org/content/10.1...

Fabulous Oli!

EU and Australia open talks on closer research partnership through Horizon Europe The European Commission and the Australian Government have begun discussing their scientific cooperation through Australia’s association with Horizon Europe

EU and Australia open talks on closer research partnership through Horizon Europe poke @katequinlan.bsky.social @merlincrossley.bsky.social @marnieblewitt.bsky.social @joelpmackay.bsky.social @pseudokinase.bsky.social

www.openaccessgovernment.org/eu-and-austr...

This would be huge.

Welcome to California! Are you stopping by Berkeley while you are here?

Priya Moorjani promoted to MCB Associate Professor

Congrats to MCB's Priya Moorjani on her promotion to MCB Associate Professor of Genetics, Genomics, Evolution, and Development! 👏🥂

mcb.berkeley.edu/news-and-eve...

Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging

Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social

www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social

New software from @bronwynalucas.bsky.social Lab for high-resolution 2D template matching of macromolecules inside cells 🔍🔬. #TeamTomo 🧶🧬🧪

Special mention to @joshdcryoem.bsky.social and Matthew Giammar who have become driving forces within the project - I'm excited to see community driven open source flourish in cryo-EM, let's see where things go! 🥰

@lainahall.bsky.social contributed lamellae and images. Keep an eye out for more from her in the not to distant future

Thanks Alister! Being a part of broader comminity efforts like teamtomo.org has made it easier to build modular tools and not have to reinvent the wheel every time. Full credit to Matthew and @joshdcryoem.bsky.social

Happy for this to be out! We have documentation and tutorials here. Please give it a try and let us know what you think. lucaslab-berkeley.github.io/Leopard-EM/

This work exists as part of a broader ecosystem of modular tools in the cryo-EM community with the goal of making it easy for new users and developers to customize for their specific application. Especially in the early days of in situ structural biology, rapid iteration is important!

@joshdcryoem.bsky.social got us connected with #teamtomo, worked alongside Matthew to contribute to the code, built the simulator and adapted Leopard-EM to perform pixel size optimization and constrained search.

This project was driven by Comp Bio PhD student Matthew Giammar who designed the structure and wrote most of the code. He separated the core functions from the user facing programs to make it easy to build new functionality with minimal changes to the user interface.

Leopard-EM: An extensible 2DTM package to accelerate in situ structural biology The ability to generate high-resolution views of cells with cryogenic electron microscopy (cryo-EM) can reveal the molecular mechanisms of biological processes in their native cellular context. The re...

First pre-print from the Lucas Lab led by Matthew Giammar and @joshdcryoem.bsky.social online today! We develop a new, extensible Python implementation of 2DTM and apply it to build pixel size optimization, constrained search and characterize molecular motions in situ www.biorxiv.org/content/10.1...

Congratulations Francis!!

To tilt or not to tilt? Strategies for in situ cryo-EM data collection Recent breakthroughs in single-particle cryogenic electron microscopy (cryo-EM) and protein structure prediction have transformed our ability to resol…

If you are going to M&M, also see postdoc @joshdcryoem.bsky.social's poster about tilted vs untilted data collection for in situ structural biology www.sciencedirect.com/science/arti....

PhD student @lainahall.bsky.social will be giving a talk about our measurements of FIB-milling damage in cryo-lamellae (with new data!) at M&M on Tuesday next week.

Reducing the effects of radiation damage in cryo-EM using liquid helium temperatures | PNAS The physical limit in determining the atomic structure of biological molecules is radiation damage. In electron cryomicroscopy, there have been num...

@joshdcryoem.bsky.social will also be giving a talk about his prior work as a PhD student with Chris Russo developing strategies to take advantage of the reduced radiation damage at liquid Helium temperatures for cryo-EM. You can find their paper here: www.pnas.org/doi/abs/10.1...

To tilt or not to tilt? Strategies for in situ cryo-EM data collection Recent breakthroughs in single-particle cryogenic electron microscopy (cryo-EM) and protein structure prediction have transformed our ability to resol…

If you are going to M&M, also see postdoc @joshdcryoem.bsky.social's poster about tilted vs untilted data collection for in situ structural biology www.sciencedirect.com/science/arti....

PhD student @lainahall.bsky.social will be giving a talk about our measurements of FIB-milling damage in cryo-lamellae (with new data!) at M&M on Tuesday next week.

Several Lucas lab members giving talks and posters at meetings this summer! If you are going to ACA2025 this week, see graduate student Matthew Giammar talk about his work building an extensible software architecture for 2DTM in Python (pre-print dropping soon) @acastructuralsci.bsky.social

Thanks! You are spot on. We are working on other strategies for applying constraints but for now that is the only way to do it.

With appropriate constraints in location and orientation, and a well structured 25 kDa. Yes.

I am checking in to share this review in case you missed it. This is the first manuscript out of the lab, written together with postdoc @joshdcryoem.bsky.social, and I think it is an important starting point for designing in situ structural biology experiments

Thank you Alexis!