It was a pleasure hosting 𝗜𝘃𝗮𝗻𝗮 𝗧𝗶𝗺𝗸𝗼𝘃𝗮 (Center for Interdisciplinary Sciences at Pavol Jozef Šafárik University in Košice, Slovakia) at Loschmidt Labs! Ivana worked with David Kovar, mastering ultrasensitive fluorescence assays 🔦🧪— skills she'll use back home.

📷 Ivana Timkova

Celebrating Loschmidt Labs' recent achievements:
- Congrats to Daniel Pluskal (Government Prize) & Martin Sitte (Best Poster)
- Kaja Sedlackova delivered her first presentation at the congress
- Our research has been accepted in top journals
- All instruments from the #CLARA project are installed

We successfully held our 𝗼𝘂𝘁𝗿𝗲𝗮𝗰𝗵 𝘀𝗲𝗺𝗶𝗻𝗮𝗿 on October 10, 2025, at St. Anne's University Hospital in Brno! The day featured excellent talks and deep discussions on the progress of the #CLARA project. We wrapped up with fun team-building at Game Center, followed by a great gathering at Pivní Burza 🍻.

Hear What Participants Say: 🗣️
📌 "This was the most informative course I have ever been to... I now have a solid foundation for my future work in enzyme and protein design."
📌 "This course is a must-attend for non-bioinformaticians (like me) looking to break into computational protein engineering!"

Registration is now open for the 𝟳𝘁𝗵 𝗛𝗮𝗻𝗱𝘀-𝗼𝗻 𝗖𝗼𝗺𝗽𝘂𝘁𝗮𝘁𝗶𝗼𝗻𝗮𝗹 𝗘𝗻𝘇𝘆𝗺𝗲 𝗗𝗲𝘀𝗶𝗴𝗻 𝗖𝗼𝘂𝗿𝘀𝗲! 🚀

Ready to become a protein design wizard? 🧙‍♂️🧙‍♀️ This course gives you hands-on experience and a solid foundation, regardless of your background.

Secure your spot:
🔗 loschmidt.chemi.muni.cz/academy/

Excited to share our new article in @febsopenbio.bsky.social!

We created optimized biochemical tests 🧪 to rapidly screen and validate clot-dissolving drugs (for stroke, pulmonary embolism, etc.).

🔗 febs.onlinelibrary.wiley.com/doi/10.1002/...

Zuzana Zelenková, Tereza Patlejchová a Daniel Pluskal jsou čerstvými držiteli Ceny vlády nadanému studentovi Náměstek ministra pro vědu, výzkum a inovace Pavel Doleček předal v úterý 30. září v prostorách Hrzánského paláce v Praze Cenu vlády nadanému studentovi za rok 2024. Cena vlády je udělována ve třech k...

More here:
vlada.gov.cz/cz/media-cen...

The 𝗚𝗼𝘃𝗲𝗿𝗻𝗺𝗲𝗻𝘁 𝗔𝘄𝗮𝗿𝗱 𝗳𝗼𝗿 𝗧𝗮𝗹𝗲𝗻𝘁𝗲𝗱 𝗦𝘁𝘂𝗱𝗲𝗻𝘁𝘀 𝟮𝟬𝟮𝟰 goes to our doctoral student, Daniel Pluskal! Congratulations to Daniel and his supervisor, Martin Marek, on this achievement! 👏

📷 Office of the Government of the Czech Republic

🔗 Course: loschmidt.chemi.muni.cz/academy/
📸 Photos: loschmidt.chemi.muni.cz/academy/foto...
🧬 Protein Engineering Portal: loschmidt.chemi.muni.cz/portal/
🏛 Loschmidt Labs: loschmidt.chemi.muni.cz/peg/

✨ Exciting News! ✨ We are thrilled to invite you to the 𝟳𝘁𝗵 𝗛𝗮𝗻𝗱𝘀-𝗼𝗻 𝗖𝗼𝗺𝗽𝘂𝘁𝗮𝘁𝗶𝗼𝗻𝗮𝗹 𝗘𝗻𝘇𝘆𝗺𝗲 𝗗𝗲𝘀𝗶𝗴𝗻 𝗖𝗼𝘂𝗿𝘀𝗲!
📅 9–13 February 2026 | 🌐 Online
🔔 Registration opens on 14 October!
🚀 This interactive 5-day training blends essential enzyme engineering theory with hands-on practice using cutting-edge tools.

𝗡𝗼𝗰 𝘃𝗲̌𝗱𝗰𝘂̊ 𝟮𝟬𝟮𝟱 na téma Bohatství 💰 je za námi a rozhodně se vydařila!
Děkujeme všem návštěvníkům, kteří se u nás zastavili, vyzkoušeli si naše experimenty a ptali se na to, co je zajímalo. ⚗️🧪🔬 Byla to skvělá příležitost ukázat, že věda může být nejen poučná, ale i zábavná! ✨
Těšíme se zase příště!

Quantum computing for faster enzyme discovery and engineering - Nature Catalysis Quantum computing is a promising technology to solve complex challenges that would take classical computers an impractical amount of time. This Perspective discusses the current state of quantum computing and possible applications in enzyme engineering and biocatalysis.

Read the full perspective here: www.nature.com/articles/s41...

Our article, "𝗤𝘂𝗮𝗻𝘁𝘂𝗺 𝗖𝗼𝗺𝗽𝘂𝘁𝗶𝗻𝗴 𝗳𝗼𝗿 𝗙𝗮𝘀𝘁𝗲𝗿 𝗘𝗻𝘇𝘆𝗺𝗲 𝗗𝗶𝘀𝗰𝗼𝘃𝗲𝗿𝘆 𝗮𝗻𝗱 𝗘𝗻𝗴𝗶𝗻𝗲𝗲𝗿𝗶𝗻𝗴," accepted by 𝗡𝗮𝘁𝘂𝗿𝗲 𝗖𝗮𝘁𝗮𝗹𝘆𝘀𝗶𝘀! 🥳 This perspective co-authored with P. Kouba & J. Sivic (CIIRC CTU in Prague), explores how quantum algorithms can tackle biocatalysis challenges that are impossible for classical computing.

Univerzitní kampus Bohunice | Noc vědců Noc, kdy vám věda a vědci ukážou svou pravou tvář

📍 Univerzitní Kampus, Bohunice, budova C13 | ⏰ 17-23 h
www.nocvedcu.cz/misto/562-un...

Už dnes! 𝗡𝗼𝗰 𝘃𝗲̌𝗱𝗰𝘂̊ je tady! 🔬

Přijďte poznat neviditelný svět! Chcete zjistit, jak vypadají miliardy bakterií 🦠, které nosíte na sobě? Ukážeme vám, jak si je vypěstovat z vlastního těla! Máme i neobvyklou ochutnávku toho, čím se mikrobi živí.

Přijďte se podívat, ochutnat a zkusit! Těšíme se! 😉

𝗡𝗼𝗰 𝘃𝗲̌𝗱𝗰𝘂̊ 𝟮𝟬𝟮𝟱 - už tento pátek!
Nezapomeňte se zastavit do Loschmidtových laboratoří v Univerzitním kampusu v Bohunicích. Dozvíte se, proč světluška svítí 💡, jak se vyrábí umělé maso 🥩, nebo si uděláte otisk prstu na živnou půdu 🧫. Těšíme se na Vás 𝟮𝟲.𝟵. 𝗼𝗱 𝟭𝟳 𝗵𝗼𝗱𝗶𝗻! 🕓

We recently said goodbye to Anthony Legrand, moving on from his postdoctoral research role at LL. We celebrated his time with a Hawaiian-themed barbecue. We're grateful for his contributions and wish him the best in his future endeavours!

📸 Karolina Sedlackova

Our new article, focusing on the effects of N-terminal His-tagging on staphylokinase (SAK) variants, has been accepted by the 𝗜𝗻𝘁𝗲𝗿𝗻𝗮𝘁𝗶𝗼𝗻𝗮𝗹 𝗝𝗼𝘂𝗿𝗻𝗮𝗹 𝗼𝗳 𝗕𝗶𝗼𝗹𝗼𝗴𝗶𝗰𝗮𝗹 𝗠𝗮𝗰𝗿𝗼𝗺𝗼𝗹𝗲𝗰𝘂𝗹𝗲𝘀!

🔗 Read the full article here:
www.sciencedirect.com/science/arti...

Find out more about the symposium on their website: htcd2026.sciencesconf.org

𝗭𝗯𝘆𝗻𝗲𝗸 𝗣𝗿𝗼𝗸𝗼𝗽 is a plenary speaker at the 𝗛𝗶𝗴𝗵-𝗧𝗵𝗿𝗼𝘂𝗴𝗵𝗽𝘂𝘁 𝗖𝗮𝘁𝗮𝗹𝘆𝘀𝘁𝘀 𝗗𝗲𝘀𝗶𝗴𝗻 (#𝗛𝗧𝗖𝗗) 𝗶𝗻𝘁𝗲𝗿𝗻𝗮𝘁𝗶𝗼𝗻𝗮𝗹 𝘀𝘆𝗺𝗽𝗼𝘀𝗶𝘂𝗺 in Lille, France! Don’t miss his lecture on the latest advances in microfluidics combined with machine learning for discovering novel biocatalysts on March 30-31, 2026!

🔥Shout-out to everyone who taught at our Young Developmental Biologist Workshop @harnoslab.bsky.social, Pavel Kohout from @loschmidtlab.bsky.social, Viktória Parobková from @ceitec.eu, Irina Poverennaya from @meduniwien.ac.at, and Petr Vodička & David Drutovič from IAPG @czechacademy.bsky.social

The new semester is here, and with it come courses taught by experts from Loschmidt Laboratories!

Join us for the 𝗟𝗶𝗳𝗲 𝗦𝗰𝗶𝗲𝗻𝗰𝗲 𝗦𝗲𝗺𝗶𝗻𝗮𝗿 𝗦𝗲𝗿𝗶𝗲𝘀 lecture featuring our guest speaker, Prof. 𝗠𝗮𝗿𝗰𝗼 𝗙𝗿𝗮𝗮𝗶𝗷𝗲 from the University of Groningen.

He will discuss his research on the enzymology of redox enzymes, enzyme discovery & engineering, and biocatalysis.

Make sure to save the date: 𝗡𝗼𝘃𝗲𝗺𝗯𝗲𝗿 𝟲𝘁𝗵, 2025.

LL successfully participated in the 𝗕𝗶𝗼𝗰𝗵𝗲𝗺𝗶𝘀𝘁𝗿𝘆 𝗖𝗼𝗻𝗴𝗿𝗲𝘀𝘀 𝟮𝟬𝟮𝟱!

👉 Karolina Sedlackova and Zbynek Prokop delivered great talks, and Martin Marek co-chaired the "Enzymes" session.

👉 Daniel Pluskal and Martin Sitte presented posters, and Martin received the 𝗕𝗲𝘀𝘁 𝗣𝗼𝘀𝘁𝗲𝗿 𝗔𝘄𝗮𝗿𝗱! 🏆

📸 Martin Marek

Our researchers 𝗔𝗹𝗮𝗻 𝗦𝘁𝗿𝘂𝗻𝗴𝗮, 𝗛𝗮𝗻𝗮 𝗙𝗮𝗹𝗱𝘆𝗻𝗼𝘃𝗮, and 𝗠𝗶𝗰𝗵𝗮𝗹 𝗩𝗮𝘀𝗶𝗻𝗮 are at ETH Zürich developing new lab-on-a-chip methods to improve the enzyme staphylokinase for treating ischemic stroke. A big thank you to Stavros Stavrakis and Eunhee Cho for the collaboration!

📸 Michal Vasina & Hana Faldynova

Our new paper is out in 𝗔𝗖𝗦 𝗖𝗵𝗲𝗺𝗶𝗰𝗮𝗹 𝗡𝗲𝘂𝗿𝗼𝘀𝗰𝗶𝗲𝗻𝗰𝗲! Did you know that certain pathogens might influence the development of #Alzheimer's Disease? Our research explores this link by looking at how proteins from pathogens affect Aβ aggregation.
Read the article: www.sciencedirect.com/org/science/...

We hosted 𝗔𝗿𝗺𝗲𝗻 𝗦𝗮𝗿𝗴𝘀𝘆𝗮𝗻 from the 𝗦𝗰𝗶𝗲𝗻𝘁𝗶𝗳𝗶𝗰 𝗮𝗻𝗱 𝗣𝗿𝗼𝗱𝘂𝗰𝘁𝗶𝗼𝗻 𝗖𝗲𝗻𝘁𝗿𝗲 "𝗔𝗿𝗺𝗯𝗶𝗼𝘁𝗲𝗰𝗵𝗻𝗼𝗹𝗼𝗴𝘆" 𝗡𝗔𝗦 𝗥𝗔, who spent 2 months with us developing amidohydrolase variants using our HotSpot Wizard & FireProtASR software. His training was made possible with the Young Scientists Support Program and COZYME - COST.

Members of the LL theoretical teams recently attended the #PragueBioML, hosted by @iocbprague.bsky.social.
👉 This two-day event brought together leading researchers and professionals to discuss the latest advancements in machine learning and data-driven research in biochemistry 💻 📅.

📸 Jan Mican

Sun, sand, and friends! 🌞 🏐 What a perfect way to spend a Friday afternoon — playing beach volleyball at Riviera in Brno.
A huge thanks to Martin Sitte for organising such a great event!

📸 Irena Halikova

Decoding Protein Stabilization: Impact on Aggregation, Solubility, and Unfolding Mechanisms Modern computational tools can predict the mutational effects on protein stability, sometimes at the expense of activity or solubility. Here, we investigate two homologous computationally stabilized haloalkane dehalogenases: (i) the soluble thermostable DhaA115 (Tmapp = 74 °C) and (ii) the poorly soluble and aggregating thermostable LinB116 (Tmapp = 65 °C), together with their respective wild-type variants. The intriguing difference in the solubility of these highly homologous proteins has remained unexplained for three decades. We combined experimental and in-silico techniques and examined the effects of stabilization on solubility and aggregation propensity. A detailed analysis of the unfolding mechanisms in the context of aggregation explained the negative consequences of stabilization observed in LinB116. With the aid of molecular dynamics simulations, we identified regions exposed during the unfolding of LinB116 that were later found to exhibit aggregation propensity. Our analysis identified cryptic aggregation-prone regions and increased surface hydrophobicity as key factors contributing to the reduced solubility of LinB116. This study reveals novel molecular mechanisms of unfolding for hyperstabilized dehalogenases and highlights the importance of contextual information in protein engineering to avoid the negative effects of stabilizing mutations on protein solubility.

Our new research explores 𝘁𝘄𝗼 𝗰𝗼𝗺𝗽𝘂𝘁𝗮𝘁𝗶𝗼𝗻𝗮𝗹𝗹𝘆 𝘀𝘁𝗮𝗯𝗶𝗹𝗶𝘀𝗲𝗱 𝗲𝗻𝘇𝘆𝗺𝗲𝘀 to understand why one remains soluble while the other aggregates. We used experimental techniques 🥼 🧪 and molecular dynamics simulations 💻 to identify the key factors that lead to reduced solubility.

pubs.acs.org/doi/10.1021/...