Sam Daly

Excited to share some recent work tracking the 3D movement of individual molecules inside the organelles of living cells (💡+ 🦠 + ⚽️💨) with the best team @joechambers.bsky.social
@jamesdmanton.bsky.social
@jsbeckwith.bsky.social @dgershlick.bsky.social @stevethechemist.bsky.social

Big big thanks to all CMM participants & sponsors #CMM2025

A short break while I present and chair a session… and we’re back! Now with an open table discussion on: MICROSCOPY! Chaired by @jsbeckwith.bsky.social, with great insights from @jamesdmanton.bsky.social and @stevethechemist.bsky.social ! #CMM2025

Thank you!

Next up we have Bin Fu discussing analysis methods for 3D imaging and then Yuetong Jia on their autofocus method for fluorescence microscopy! #CMM2025

CMM 2025 day 1

Day 2 of #CMM2025! We're kicking off the first session with our third invited speaker Prof Tuomas Knowles presenting on "Probing protein self-assembly and phase transitions using microfluidics". @cmicrosocpymeeting.bsky.social

And it’s a wrap for Day 1 of @cmicrosocpymeeting.bsky.social! Loads of fantastic talks, looking forward to Day 2! But for now: PUB! #CMM2025

And our second keynote/final talk of the day, Siân Culley (@superresolusian.bsky.social) from KCL! Talking here about "The Good, The Bad and The Ugly in Fluorescence Microscopy" #CMM2025

Next up, "Resolving Parkinson's Disease Pathology with Spatial Proteomics" from Marc Oeller. Literally cutting and sticking on a cellular level! #CMM2025

We're now well into the second session of the first day, looking at further method development! We're listening to James Simon who is discussing the use of conductance microscopy in battery research, and Stefan Oswald on tracking battery ions with microscopy! #CMM2025

a man in a tie is making a funny face with his hands in the air and the words `` so excited ! '' ALT: a man in a tie is making a funny face with his hands in the air and the words `` so excited ! ''

Wooo!

A room full of microscopy enthusiasts, Cambridge Microscopy Meeting 2025. #CMM2025

On to Session 1 now! "Method Development (Advanced illumination)", kicking off with Rebecca McClelland on accessible SIM, then Jacob Lamb on Oblique Plane Microscopy! #CMM2025

The lecture theatre is filling up with people ready for CMM2025!

Let's get the hashtag #CMM2025 for CMM2025 (@cmicrosocpymeeting.bsky.social) to get the discussion rolling!

And we’re off! Starting with Steven Lee’s opening remarks before handing over the Dr Lisa-Maria Needham to talk about label free detection of biomolecules!

We’re busy setting up for Cambridge Microcopy Meeting 2025 this week! Looking forward to exciting talks, but first setting up space for sponsor exhibits! #cmm2025 @cmicrosocpymeeting.bsky.social @jsbeckwith.bsky.social @chemlibcam.bsky.social

We’re thrilled to announce our exhibition, Space: Could Life Exist Beyond Earth?, opening 16 May 2025. 🚀

Rooted in the latest cutting-edge scientific research from the Museum, the exhibition will uncover evidence that life could exist beyond our planet.

MINFLUX achieves molecular resolution with minimal photons - Nature Photonics The authors review MINFLUX super-resolution microscopy, outlining its advantages and limitations, recent progress, and an outlook for future developments.

By putting a donut between our faces and each article we read, we were able to extract more information than ever before about MINFLUX. You can read the new review article from @maxperutzlabs.bsky.social by Lukas Scheiderer, myself, and @jonasries.bsky.social at www.nature.com/articles/s41...

High-density volumetric super-resolution microscopy - Nature Communications Current approaches for volumetric super-resolution microscopy can yield large and complex PSF spatial footprints. Here, the authors show a super-resolution microscopy approach using a hexagonal microl...

Excited to see that my first, first author paper out of my PhD was selected among the Top 25 Physics Research papers of 2024 by Nature Communications! @naturecomms.bsky.social www.nature.com/articles/s41...

If you're interested in advanced 3D super-resolution microscopy then there is an exciting new job opportunity in TheLeeLab here in Cambridge.

Happy to answer any questions!

🔬🧪🦠

www.jobs.cam.ac.uk/job/50547/

Cambridge risks losing ‘unbelievable talent’ amid PhD funding cut Warning by vice-chancellor Deborah Prentice comes as ‘Silicon Valley’ planned between Oxford and Cambridge

“I make a lot less than I made in the US, and I wasn’t even head of an institution in the US, right?”

That yearly salary could fund over 30 PhDs… that’s our problem.

www.theguardian.com/education/20...

does anyone know of good repositories for (e.g.) nice scalable vector graphics of a microscope objective? Or have a nice svg they'd be willing to part with? @hohlbeinlab.bsky.social @jamesdmanton.bsky.social @samofmidworld.bsky.social #chemsky #microscopy

First panic attack of the year! Managed to make it until the 6th Jan—good going Sam!

Was I there? Yes. Am I still immensely excited for 4:10? Yes!

Festive science activity

Have a go at these 24 FREE #FestiveScience activities from #DrJoScience and share what you get up to!
#ScienceIsEverywhere #BeCurious #STEMActivities #Advent #AdventCalendar #PrimaryScience
bio.link/DrJoScience

Single molecule nerds come work with us! www.jobs.cam.ac.uk/job/49742/

3D Super-Resolution Imaging of PSD95 Reveals an Abundance of Diffuse Protein Supercomplexes in the Mouse Brain PSD95 is an abundant scaffolding protein that assembles multiprotein complexes controlling synaptic physiology and behavior. Confocal microscopy has previously shown that PSD95 is enriched in the postsynaptic terminals of excitatory synapses and two-dimensional (2D) super-resolution microscopy further revealed that it forms nanoclusters. In this study, we utilized three-dimensional (3D) super-resolution microscopy to examine the nanoarchitecture of PSD95 in the mouse brain, characterizing the spatial arrangement of over 8 million molecules. While we were able to identify molecular arrangements that have been previously reported, imaging in 3D allowed us to classify these with higher accuracy. Furthermore, 3D super-resolution microscopy enabled the quantification of protein levels, revealing that an abundance of PSD95 molecules existed outside of synapses as a diffuse population of supercomplexes, containing multiple copies of PSD95. Further analysis of the supercomplexes containing two units identified two populations: one that had PSD95 molecules separated by 39 ± 2 nm, and a second with a separation of 94 ± 27 nm. The finding that there exists supercomplexes containing two PSD95 units outside of the synapse suggests that supercomplexes containing multiple protein copies assemble outside the synapse and then integrate into the synapse to form a supramolecular nanocluster architecture.

First paper out of my postdoc has just dropped! We map the 3D spatial relationship of PSD95 in mouse brain using DHPSF super-resolution microscopy… and in just enough time for the last #FluorescenceFriday before Christmas! 🎄 pubs.acs.org/doi/10.1021/...

Lead the way!

A PowerPoint slide saying ‘welcome! UK membrane trafficking meeting’

Excited to attend (my first proper biology conference at) UK Membrane Trafficking Meeting! With @dgershlick.bsky.social @awolowczyk.bsky.social @joechambers.bsky.social