Fromme Lab

Check out our new biosensor technology to study DDR kinase signaling: ProKAS.

We combine:
-proteomics
-engineered peptide sensors
-a new concept of amino acid barcodes

ProKAS tracks kinase signaling with spatial resolution and produces highly quantitative data.

Just published today: rdcu.be/ePNo0

Sarah and the Cohen lab are doing amazing work!!!

Important article from @marymunson4.bsky.social @joann-trejo.bsky.social @needhibhalla.bsky.social

🎉 Huge congrats to Maya Schuldiner from the Weizmann Institute (Israel) for the 🏅 Otto Warburg Medal 2026!
Her work on how proteins find their way to organelles and how these organelles talk to each other has reshaped how we think about cells 🧬✨
#OWM #WeizmannInstitute @elsevierconnect.bsky.social

Science history: Scientists use 'click chemistry' to watch molecules in living organisms — Oct. 23, 2007 Carolyn Bertozzi and colleagues laid out a way to make paradigm-shifting "click-chemistry" compatible with living cells, opening up a window into living organisms.

Move over, mole day: happy #bioorthogonal copper-free click chemistry day to all who celebrate! 🎉 www.livescience.com/chemistry/sc... @carolynbertozzi.bskyverified.social

The Golgi vesicle tether p115/USO1 can bind directly to the ER exit site organiser Sec16A Newly-made secretory and membrane proteins exit the endoplasmic reticulum (ER) in COPII vesicles that form at specialised ER exit sites. These exit sites are typically near to the early Golgi compartm...

New preprint from Sean Munro @cellbiol-mrclmb.bsky.social! Golgi tether p115 directly binds Sec16A at ER exit sites. Mutations blocking this binding reduce secretion. A rich new mechanistic insight into physical coupling of ERES–ERGIC/Golgi.
www.biorxiv.org/content/10.1...

Thanks to the Cornell Chronicle and @research-and-innovation.cornell.edu for highlighting our new small molecules targeting lipid-binding proteins in cancer! news.cornell.edu/stories/2025...

Postdoctoral Research Assistant - MCDB - UOD2016 Closing date: Thursday 6 November 2025, 23:59

Attention membrane traffickers! I'm recruiting a post-doc to my lab in Dundee. We're dissecting the functions of intrinsically disordered domains of COPII coat proteins. We think they control timing of coat assembly and morphology of carriers.

www.dundee.ac.uk/work-for-us/...

Discovery, Optimization, and Anticancer Activity of Lipid-Competitive Pleckstrin Homology Domain-Containing Family A Inhibitors Phosphoinositide signaling is a major cellular mechanism controlling cancer cell viability, proliferation, and survival. Yet, inhibition of lipid kinases that produce oncogenic phosphoinositides has afforded only a limited number of efficacious drugs attributed in large part to on-target toxicity resulting from the pleiotropic effects of these signaling lipids. Targeting the specific phosphoinositide effector pathways via competitive inhibitors of phosphoinositide-recognizing pleckstrin homology (PH) domains represents a relatively unexplored means to achieve greater specificity. Herein, we present the discovery from in silico screening, structure–activity relationship (SAR) optimization, and cellular characterization of novel phosphoinositide-competitive inhibitors of the pleckstrin homology domain-containing A (PLEKHA) family. These compounds induce cytotoxic effects in BRAF and NRAS mutant melanoma cells, consistent with on-target inhibition, and the most potent compound is activated by endogenous esterase activity, suggesting that prodrug esters represent a viable strategy for targeting the phosphoinositide-binding pockets of the PLEKHA family of PH domains.

A while back we found that the lipid-binding protein PLEKHA4 boosts Wnt/β-catenin signaling and drives melanoma growth in vivo. Now, we (Nathan Frederick) identify small-molecule inhibitors of PLEKHA4 & related proteins with anticancer activity in vitro! pubs.acs.org/doi/10.1021/....

The mechanistic basis of cargo selection during Golgi maturation Resident proteins of the Golgi recycle in vesicles and the protein GOLPH3 enables the COPI vesicle coat to accomplish this.

The mechanistic basis of cargo selection during Golgi maturation. From Rebecca Taylor, @katciazynska.bsky.social, @jggkaufman.bsky.social & @grigorytagiltsev.bsky.social at @mpibiochem.bsky.social with David Owen and Sean Munro's group @cellbiol-mrclmb.bsky.social | www.science.org/doi/10.1126/...

How do cells sense & respond to lipid imbalances? What happens when a disease-relevant enzyme is blocked? Shiying Huang investigates phosphoinositide lipids with the Balla lab & discovers an integrated cellular response that boosts alternate lipid synthesis pathways! www.biorxiv.org/content/10.1...

Phosphatidylserine and RhoB connect phosphatidylinositol 4-phosphate and phosphatidic acid metabolism at the plasma membrane https://www.biorxiv.org/content/10.1101/2025.09.30.679611v1

Identification of a RAB32-LRMDA-Commander membrane trafficking complex reveals the molecular mechanism of human oculocutaneous albinism type 7 Nature Communications - Membrane trafficking is essential for organelle biogenesis. Here, the authors discover an alternative assembly of the Commander trafficking complex and show its role in...

Latest endosomal publication with @brettcollins.bsky.social: Rebeka Butkovic's beautiful work identifying a new Commander assembly, the RAB32-LRMDA-Commander, and its role in melanosome biogenesis. Reveals molecular mechanism of human oculocutaneous albinism type 7. www.nature.com/articles/s41...

Tenure Track Faculty - Molecular Biology and Genetics - Ithaca, New York job with Cornell University | 675236 Tenure Track Faculty, Molecular Biology and Genetics Weill Institute for Cell and Molecular Biology Department of Molecular Biology and Genetics Co...

We are hiring, come be our colleague!

jobs.sciencecareers.org/job/675236/t...

COPI-dependent intra-Golgi recycling at an intermediate stageof cisternal maturation https://www.biorxiv.org/content/10.1101/2025.09.20.677526v1

@weillinstitute.bsky.social
first Trainee Works in Progress Seminar of the fall semester with Mingqin Chang of @fromme-lab.bsky.social

The Eukaryotic homology search complex distorts donor DNA structure to probe for homology Homologous recombination (HR) is a DNA double-strand break repair pathway that facilitates genetic exchange and protects damaged replication forks during DNA synthesis. As a template-based repair proc...

Just throwing this out there as a significant conceptual advance in the field

www.biorxiv.org/content/10.1...

Wonderful to see our paper on the #organelle signatures of #neurons and #astrocytes out in final form - congratulations, Shannon Rhoads and team!🎉 t.co/BPxKlbU6Ou

Dylan's study is now out in @jacs.acspublications.org! Key new experiments by Yuan-Ting Cho support a model for why a rare three-tailed lipid, NAPE, might accumulate during stroke & heart attack: as a protective response to promote lactate export as cells shift to glycolysis doi.org/10.1021/jacs...

Mechanistic adaptation of the metazoan RabGEFs Mon1-Ccz1 and Fuzzy-Inturned The molecular comparison of related RabGEFs reveals adaptation mechanisms of a functional module for specific cellular tasks.

Thrilled to share our newest publication in Science Advances!
We uncovered how two RabGEF complexes — Mon1-Ccz1 and Fuzzy-Inturned — adapt to regulate distinct Rab GTPases despite their structurally conserved catalytic core.
www.science.org/doi/full/10....

Plant cell wall mechanics inform how to grow usable forms | Cornell Chronicle Cornell scientists work toward engineering plants and other organisms to grow into usable, biodegradable forms.

New research from @roederlab.bsky.social: Plant cell wall mechanics inform how to grow usable forms news.cornell.edu/stories/2025...

We are proud to announce our newest adventure!

Introducing the Diffuse Project, a community-driven effort to predict protein dynamics using the subtle diffuse scattering signal from protein crystals. This collaboration marks a new era for the Ando lab!

as.cornell.edu/news/philant...

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Very excited to announce that my first, first author paper, is now on BioRxiv! In this paper we unambiguously show #COPII coated vesicles in unperturbed human cells for the very first time!!

Make my day and check it out!
www.biorxiv.org/content/10.1...

#Cryo-ET #TeamTomo #Cryo-CLEM ❄️🔬❄️🔬❄️🔬❄️🔬

@cellbiol-mrclmb.bsky.social @briggsgroup.bsky.social

Wow! GOLPH3 binding to COPI is competitive with binding of canonical -KKXX cargos, providing an elegant mechanism for differential sorting of COPI cargos during Golgi maturation. Congratulations to all the authors on an impressive and important study:

www.biorxiv.org/content/10.1...

Our neighbors are the best!!!

We love you, Mariena❣️

I shall feast on the lipid remnants of my enemies and see their membranes ground to dust!!

Happy #GolgiDay to all who celebrate!! Made a Golgi “stack” to celebrate 😉🥞

(featuring my beloved and elusive Golgi tubules..!)

Crazy how sharing a new paper at this moment feels insignificant in relation to all that is happening, but also feels important to keep doing our jobs and be proud of the work we all do