Dan Levy 🤔🏊☕️🚴🎶 👟

2) We also demonstrate diverse responses, primarily decreased macrophage signaling following cortical spreading depression. Tour de force imaging and data analysis work led by postdoc Simone Carneiro-Nascimento.

1) Using 2P imaging of meningeal macrophages in awake Pf4:GCaMP6s mice, we show diverse Ca2+ signaling at steady state, including very intriguing functional coupling between dural perivascular macrophages and dura vasomotion.

Meningeal macrophages exhibit diverse calcium signaling at steady-state and in response to aberrant cortical hyperexcitability in awake mice The meninges, which envelop and protect the brain, host a large number of resident macrophages that play a crucial role in regulating homeostasis and neuroinflammation. Intracellular Ca2+ signaling me...

A new preprint out from the Levy lab, adding knowledge to the exciting world of brain border macrophages and neuroimmunology.
www.biorxiv.org/content/10.1...

Meningeal macrophages exhibit diverse calcium signaling at steady-state and in response to aberrant cortical hyperexcitability in awake mice https://www.biorxiv.org/content/10.1101/2025.10.01.679335v1

Couldn't find any pot of gold.

Gone for some summer hiking.

Dear SDB Members, NIH is planning to cap APC (Article Processing Charges) from grant funds for publications, starting in January 2026. https://grants.nih.gov/grants/guide/notice-files/NOT-OD-25-138.html Proposed Policy Options for Keeping Publication Costs Reasonable: Option 1: Disallow all publication costs. Option 2: Set a $2,000 limit on allowable costs per publication. Option 3: Set a $2,000 limit on allowable costs per publication and allow up to $3,000 when peer reviewers are compensated. Option 4: Set an 0.8% limit on the total amount of an award that can be spent on publication costs, up to $20,000. For example, $250,000 direct cost grant = $2,000 per year for publications. Option 5: Set a $6,000 limit on both the per publication cost and the total amount of an award (0.8% up to $20k) that can be spent on publications. The NIH is requesting comments by Sept 15. It is also possible to contribute comments that are not about any of the 5 options. Comments should be submitted electronically to the following webpage: https://osp.od.nih.gov/comment-form-maximizing-research-funds-by-limiting-allowable-publishing-costs/. Responses are voluntary and may be submitted anonymously. The SDB encourages you to submit your opinions on this important issue. Best, Richard Behringer President, SDB

NIH is considering changes in supporting publication costs--some ideas are quite radical and would endanger our ability to publish at all. I don't love the cots from the big for-profits but it does cost$ to run a journal. Please repost. Comment at:
osp.od.nih.gov/comment-form....

The Dissection and Reconstruction of Music From the Past As Performed By the Inmates of Lalo Schifrin's Demented Ensemble As a Tribute To the Memory of the Marquis De Sade by Lalo Schifrin on Apple Mu... Album · 1966 · 10 Songs

My first encounter with Lalo Schifrin. RIP genius.
music.apple.com/us/album/the...

Who else is joining tomorrow for lunch?

Paper accepted, will soon have comments added.

Friday, Judge ruled in Harvard's favour, Paper accepted after minor revisions. Can I go home now?

Cortical astrocyte activation triggers meningeal nociception and migraine-like pain - preLights Seeing stars – astrocytes in primary visual cortex drive migraine pain.

Seeing stars ✨

Astrocytes in primary visual cortex drive migraine pain.

Check out the new preLights post from Vanessa Ehlers highlighting a recent study from Dara Bree, Dan Levy (@danlevy.bsky.social) & team. #preprint

#preLight ⬇️ 👀
👉 prelights.biologists.com/highlights/c...

A distributed coding logic for thermosensation and inflammatory pain - Nature Functional imaging and multiplexed in situ hybridization were combined to investigate how trigeminal neurons encode heat and mechanical stimuli, revealing distinct cellular mechanisms for continuing p...

co-author @dimacd.bsky.social pointed out that the link was truncated in my post. Hope this works: www.nature.com/articles/s41...

Meningeal regulatory T cells inhibit nociception in female mice T cells have emerged as orchestrators of pain amplification, but the mechanism by which T cells control pain processing is unresolved. We found that regulatory T cells (Treg cells) could inhibit nocic...

Meningeal regulatory T cells inhibit nociception in female mice | @science.org www.science.org/doi/10.1126/...

Woodstock.Bio 2 & Night Science is the meeting where organizers register & pay like others! If you want to be a part of this unforeseen, unique, unbelievable, unforgettable, unpredictable & untouchable meeting, there's dozens of places left. It's 230 PAYING! Beyond that is the waiting list.

Many of us use 2p scopes to image 3D volumes of brain. But then we analyze the data plane by plane, resulting in duplicated neurons, missed neurons, and low s/n. Let's go 3D!

Suite3D: Volumetric cell detection for two-photon microscopy
by @haydari.bsky.social & team.
www.biorxiv.org/content/10.1...

Neural ensembles that encode nocifensive mechanical and heat pain in mouse spinal cord - Nature Neuroscience Zhang et al. identify unimodal neural representations in the spinal cord of cutaneous mechanical and heat stimuli gated by a shared feed-forward local inhibitory neuron type and a neural transition du...

How is the input of the different kinds of primary sensory neurons responding to heat and mechanical stimuli summarized in the spinal cord? Check out our recent study.

www.nature.com/articles/s41...

If you wish to attend a conference like no other, feed your creativity, present your research to people who don't come to your typical meetings, enjoy Prague & Bohemian woods, music, fire, some beer, then this meeting may be for you. Registration & info here: woodstock.img.cas.cz
90/230 places left

The Conference full stop

‘Travel agencies charge far more than it would cost an academic to arrange their own travel and accommodations - sometimes staggeringly more.’

I'm in.

Weird algorithm, didn't see you posting on the meeting.

It was :)

No clue

Friends! The science that we love is under threat. World-wide, there're forces seeking to make it corporate-like, focused on deliverables, or even the enemy of society. Take a stand & celebrate science & music! #theconferencetoendallconferences - Registration starts NOW!
woodstock.img.cas.cz

Do we need bear spray for the woods?

Looking forward

Scratching promotes allergic inflammation and host defense via neurogenic mast cell activation Itch is a dominant symptom in dermatitis, and scratching promotes cutaneous inflammation, thereby worsening disease. However, the mechanisms through which scratching exacerbates inflammation and wheth...

New research in Science uncovers the dual nature of scratching an itch; although it can worsen skin inflammation, it can also boost immune defenses against bacterial infections at the injury site.

Learn more: scim.ag/40RtBjF

We also think so.

IT'S HAPPENING! The Woodstock of Biology 2.0 meets Night Science @nightsciencepod.bsky.social
10-13 of June in Prague & the Woods of Bohemia...
Be there or be REJECTED. #theconferencetoendallconferences