Satpal (Sati) Jhujh

A conceptual illustration of two people talking against background of leafless trees with green vines growing inside them. Text reads: How I confronted my growing cynicism about academia—and rekindled my sense of purpose.

"… as I helped this bright young scholar rediscover hope in her journey, I rediscovered my own." #ScienceWorkingLife https://scim.ag/473CI43

1) often people now are unwilling to listen to a view or point that runs counter to what they already believe.

2) probably all bots 😂

I'm not 100% sure where I land on that tbh. While normally I think it would be good to challenge the misinformation and perhaps try to provide an opposing view there are 2 things in the way of that imo:

Science research gets more engagement on Bluesky than X, study finds Bluesky posts referencing scholarly articles ‘find substantially higher levels of interaction’ than on Elon Musk’s platform

It would seem for science and scientific discussion Bluesky is the place to be. I deleted my X accounts last summer as it was genuinely terrifying to me the level of hatred and misinformation on there.

www.theguardian.com/technology/2...

Strand- and replication timing-dependent functions of DNA polymerase η in human DNA replication and mutagenesis. Efficient eukaryotic DNA replication relies on the coordinated actions of replicative and error-prone polymerases, with the latter providing flexibility at the cost of mutagenesis. DNA polymerase η (P...

Our preprint on Pol-η usage during genome replication has been uploaded. It addresses the biased use of Pol-η in the lagging strand and late-replicating regions, which may be correlated with cancer mutagenesis.
www.biorxiv.org/content/10.1...

DDIAS is a single-stranded DNA-binding effector of the TOPBP1-CIP2A complex in mitosis https://www.biorxiv.org/content/10.1101/2025.09.09.675193v1

NIPBL dosage shapes genome folding by tuning the rate of cohesin loop extrusion Cohesin loop extrusion is a major driver of chromosome folding, but how its dynamics are controlled to shape the genome remains elusive. Here we disentangle the contributions of the cohesin cofactors ...

New preprint with @gfudenberg.bsky.social

We find the rate of cohesin loop extrusion in cells is set by NIPBL dosage and tunes many aspects of chromosome folding.

This provides a molecular basis for NIPBL haploinsufficiency in humans. 🧵👇

www.biorxiv.org/content/10.1...

DNA2 enables growth by restricting recombination-restarted replication - Nature DNA2 suppresses recombination-restarted replication and checkpoint activation at stalled forks, and its loss triggers recombination-dependent synthesis, checkpoint signalling and cell-cycle exit, high...

My first post on bsky to share our latest research in @nature.com: “DNA2 enables growth by restricting recombination-restarted replication” - defines DNA2’s essential function & elucidates links with primordial dwarfism and cancer. Thanks to the lab, collaborators & @ukri.org @acmedsci.bsky.social

Some seriously nice biochemistry and cryo-EM of FAN1-PCNA-flapDNA complexes! www.nature.com/articles/s41...

Slow RNAPII elongation enhances naive-pluripotency rewiring while preserving replication fork speed https://www.biorxiv.org/content/10.1101/2025.05.12.653265v1

3rd edition of the fantastic UK DNA replication meeting is happening next September in Edinburgh. Don't miss out 🧬🏴󠁧󠁢󠁳󠁣󠁴󠁿

www.biochemistry.org/events-and-t...

🎉 Congratulations! Great to see this out in print! 👏

Done!

Pls repost. Postdoc role on post-replicative ssDNA gap processing. Interesting models that should float replication aficionados boats. DM or c.staples@bangor.ac.uk for info. Until Dec26 but will strive for funding to provide continuity.
Fantastic place to live+work
www.jobs.ac.uk/job/DMG682/r...

Nuclear and genome dynamics underlying DNA double-strand break repair Nature Reviews Molecular Cell Biology - Changes in nuclear and genome organization promote the repair of DNA double-strand breaks and genome stability. Processes that are involved include the...

How are chromosome breaks repaired and how is their repair influenced and facilitated by nuclear and genome dynamics? Fantastic initiative and collaboration with @karimmekhail.bsky.social, @chiololab.bsky.social, and @lablegube.bsky.social. @natrevmcb.bsky.social Link to article: rdcu.be/edViW

Resolution of collapsed forks is separate from completion of DNA synthesis Replication fork collapse at single-strand DNA breaks (SSBs) poses a serious threat to genome stability. Using Xenopus egg extracts, we show that a replication fork encountering an SSB on either the l...

🧬⛓️‍ Our latest preprint examines the fate of collapsed forks in vertebrates 🐸 and our colleagues in @heathmurraylab.bsky.social asked similar questions in bacteria 🦠 to reveal how evolution shapes the response to fork collapse (see🧵)
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...

✅ Done!

TRIP13 protects pancreatic cancer cells against intrinsic and therapy-induced DNA replication stress https://www.biorxiv.org/content/10.1101/2025.01.26.634889v1

Telomeric SUMO level influences the choices of APB formation pathways and ALT efficiency https://www.biorxiv.org/content/10.1101/2025.01.16.633463v1

Okay, the list is far from complete, but it is a first attempt.☺️Here you can find our starter pack on #research organizations and #academies, in #Germany and worldwide. Who else do you think should be included? go.bsky.app/DGGtVu6

New discovery from our #CDlab reported in Cell @cp-cell.bsky.social today:
SMC motor proteins have a left-right ‘gearbox’ and can switch direction in DNA loop extrusion!

www.sciencedirect.com/science/arti...

1/

The PST repeat region of MDC1 is a tunable multivalent chromatin tethering domain https://www.biorxiv.org/content/10.1101/2025.01.10.632395v1

Identification of critical factors of the replication stress response in human cells https://www.biorxiv.org/content/10.1101/2025.01.12.632149v1

Cell cycle progression of under-replicated cells Abstract. Cell cycle checkpoints are the regulatory mechanisms that secure the strict order of cellular events for cell division that ensure genome integri

Fascinating paper! No G2/M checkpoint for under-replicated cells!
academic.oup.com/nar/article/...

Hypoxia promotes tumor immune evasion by suppressing MHC-I expression and antigen presentation | The EMBO Journal imageimageHypoxia contributes to cancer resistance to both standard therapies and immunotherapy. This work demonstrates that tumor hypoxia promotes immune evasion by autophagy-mediated downregulation ...

Great start to 2025. Tumor hypoxia promotes immune evasion by autophagy-mediated downregulation of MHC class I expression leading to reduced antigen presentation. Great work from @halaestephan.bsky.social www.embopress.org/doi/full/10....

MAPK14/p38α shapes the molecular landscape of endometrial cancer and promotes tumorigenic characteristics Using unbiased CRISPR-Cas9-based genetic screens, Joseph et al. identify MAPK14/p38α as a dependency and vulnerability of high-grade endometrial cancer (HGEC) metastatic spheroids. MAPK14/p38α critically programs the phosphoproteome, transcriptome, and metabolome of HGEC spheroids, promoting cancer stemness and tumorigenicity.

Great paper about p38 and endometrial cancer from a long-term collaborator and friend, Cyrus Vaziri, just published in Cell Reports:

www.cell.com/cell-reports...

Hi, done!

EXO1 and DNA2-mediated ssDNA gap expansion is essential for ATR activation and to maintain viability in BRCA1-deficient cells Abstract. DNA replication faces challenges from DNA lesions originated from endogenous or exogenous sources of stress, leading to the accumulation of singl

Our last article about ssDNA gaps and BRCA1 👇🏻👇🏻
doi.org/10.1093/nar/...

YouTube video by WEHImovies DNA Break Repair by Homologous Recombination (2024) Drew Berry wehi.tv

Delighted to publish my new molecular animation:

DNA Break Repair by Homologous Recombination

youtu.be/Xe-83tBcxhs

Hi, of course, it's been added 👍🏽