Luke Greenhough

Been waiting for this! Looking forward to reading it

24.06.2025 08:46 — 👍 1    🔁 0    💬 0    📌 0

Schematic illustrating the structural changes undergone by MukBEF SMC complex during DNA capture and ingestion.

How do SMC complexes capture DNA? 🧪

Led by Frank Bürmann, Jan Löwe’s & Mark Dillingham’s groups have identified the directional loading mechanism the bacterial SMC complex MukBEF uses to capture & ingest DNA, & found a phage inhibitor to this pathway.

Read more: tinyurl.com/4s8nrrkk

#LMBResearch

31.03.2025 14:40 — 👍 62    🔁 32    💬 1    📌 1

Structural mechanism of strand exchange by the RAD51 filament. Homologous Recombination (HR) preserves genomic stability by repairing double-strand DNA breaks and ensuring efficient DNA replication. Central to HR is the strand-exchange reaction taking place within the three-stranded synapsis wherein a RAD51 nucleoprotein filament binds to a donor DNA. Here we present the cryoEM structure of a displacement loop of human RAD51 that captures the synaptic state when the filament has become tightly bound to the donor DNA. The structure elucidates the mechanism of strand exchange by RAD51, including the filament engagement with the donor DNA, the strand invasion and pairing with the complementary sequence of the donor DNA, the capture of the non-complementary strand and the polarity of the strand-exchange reaction. Our findings provide fundamental mechanistic insights into the biochemical reaction of eukaryotic HR. ### Competing Interest Statement The authors have declared no competing interest.

Over five years of work has gone into our new preprint! The #cryoEM structure of a human RAD51 filament caught at the point of strand exchange 🧬
www.biorxiv.org/content/10.1...

27.03.2025 15:12 — 👍 17    🔁 6    💬 0    📌 1

Nuclear and genome dynamics underlying DNA double-strand break repair Nature Reviews Molecular Cell Biology - Changes in nuclear and genome organization promote the repair of DNA double-strand breaks and genome stability. Processes that are involved include the...

🚀 New Paper Alert! Our latest @natrevmcb.bsky.social explores how nuclear and genome organization drive DNA double-strand break repair! 🧬

📖 Free access: rdcu.be/edViW

Please 🔄 ❤️

18.03.2025 13:49 — 👍 57    🔁 24    💬 1    📌 0

Check out our review on DNA end resection! With Raphael Ceccaldi, www.nature.com/articles/s41...

25.03.2025 16:00 — 👍 66    🔁 25    💬 2    📌 2

Been excited for this for a while! Excited to chat about all things paralog related

25.03.2025 07:21 — 👍 1    🔁 0    💬 0    📌 0

Excited to see the final published form of this amazing @drewberry.bsky.social‬ animation of DNA repair go live! Watch your favourite friends BRCA1/2, RPA, RAD51, Exo1, BLM, PCNA, MRN, primase and DNA polymerase perform their recombination magic. Cool educational & scientific tool for our community.

04.12.2024 01:46 — 👍 37    🔁 8    💬 2    📌 0

Predictomes, a classifier-curated database of AlphaFold-modeled protein-protein interactions Schmid and Walter train a classifier that discerns functionally relevant structure predictions in proteome-wide protein-protein interaction (PPI) screens using AlphaFold-Multimer, and they use this co...

Very excited to share that my thesis work is out in Molecular Cell! We trained a Structure and Omics informed Classifier (SPOC) to score binary AlphaFold multimer (AF-M) predictions by structural quality and consistency with experimental omics datasets. www.cell.com/molecular-ce...

26.02.2025 18:14 — 👍 24    🔁 8    💬 3    📌 1

Thank you to Roger Greenberg and Hilda Pickett for organising a fantastic 2025 Mammalian DNA Repair GRC in Ventura, California.

Looking forward to the next time.

07.02.2025 00:37 — 👍 15    🔁 5    💬 0    📌 0