End-to-end localized deep learning for Cryo-ET
Cryo-electron tomography (cryo-ET) enables 3D visualization of cellular environments. Accurate reconstruction of high-resolution volumes is complicated by the very low signal-to-noise ratio and a rest...
I have been using CryoLithe recently as DeepDeWedge and cryoCARE erase many of the periplasmic complexes that I want to pick.
arxiv.org/abs/2501.15246
If you are unfortunate and have to manually pick particles. I have had the most success using the WARP deconvolution filter.
11.07.2025 00:01 β π 2 π 0 π¬ 1 π 0
My total reliance on Nvidia GPUs is a bit concerning.
27.05.2025 23:22 β π 0 π 0 π¬ 0 π 0
Has anyone encountered the issue of DM3 crashing and taking TIA with it during long tomo collections? If so, did you find a solution?
#Teamtomo #CryoET
30.11.2024 04:58 β π 0 π 1 π¬ 0 π 0
It seems like, no matter what technique you use, it's Fourier transforms all the way down.
27.11.2024 11:48 β π 0 π 0 π¬ 0 π 0
For tomography and STA, I feel like EMAN2 was ahead of its time. I am still confused as to why it isn't used by more people.
10.11.2024 08:32 β π 1 π 0 π¬ 0 π 0
Computational Cryo-EM/ET at Genentech
Love people, the outdoors and building stuff
Complexity is the enemy
All opinions my own
Not-for-profit publisher of Development, Journal of Cell Science, Journal of Experimental Biology, Disease Models & Mechanisms and Biology Open. Host of community sites the Node, preLights and FocalPlane. Supporting biologists and inspiring biology.
structural cell biologist @EMBL π¨π¦
a senior scientist and manager at the New York Structural Biology Center, SEMC & NCCAT - https://nccat.nysbc.org - Views are my own, Reposts/Likes do not imply endorsement.
Dpt. for #MolecularSociology @mpibp.bsky.social
https://www.biophys.mpg.de/molecular-sociology
Asst Prof of Molecular Cell Biology and Computational Biology at UC Berkeley
Building a new lab capturing close-ups of cells with electrons. lucaslab.science
Attempting to "dwell (somewhat) patiently in the variety and complexity of organisms."
Rubisco phase separation + Cryo-ET
PhD @ Engel & Hondele Lab Biozentrum Basel ππ
#TeamTomo
Research Assistant Professor at OHSU.
Structure biology| Drug resistance| Transporter| Cryo-EM| Membrane proteins|Microbiology|
Past: #UCSF, Research Specialist II | Bio5 postdoctoral fellow @uaarizona | #ICGEB #JNU #MSU
CryoEM, Structural Cell Biology. Group Leader@University of Melbourne. #NHMRC Emerging Leadership Fellow. Formerly at @Caltech, @MRC_LMB.
Staff scientist @biozentrum.unibas.ch using algorithms to look at tiny stuff inside cells, or #cryoET if you prefer. Here I talk mostly about my research. Views my own. π§π· in π¨π
Researcher in situ structural biology using CryoET (Melb, Australia). Father, Greyhound companion, coffee enthusiast, and willing but not able golfer.
Cryo-electron tomography and subtomogram averaging. Pushing buttons, turning knobs, and furiously smashing keyboard. Assistant prof at Vanderbilt University; I ask other people to pipette things, often viruses.
Try / Fail / Learn / Try again, cryoEM and all EM related things, maybe a bit of wood working / cycling / fishing
PhD student at Enninga Lab - Institut Pasteur Paris | cryo electron tomography & Mtb π§«π¬
Postdoc in the Abraham Lab @HMS | Viruses & CryoET/EM | she/they π³οΈβπ
Action is the offspring of dark pessimism.
All things #cryoEM #Electronmicroscopy at Thermo Fisher
MRC-LMB #cryoET #biofilms #S-layers #antibiotics
https://www2.mrc-lmb.cam.ac.uk/groups/bharat/
