Sarah Bigot

📢 Recrutement d’un(e) Maître(sses) de conférence en Biochimie générale, spécialité bactériologie moléculaire à MMSB

🔬Recherche
*Immunité anti-phages
*Réponse au stress, modulation de la croissance bactérienne
✉️ christophe.grangeasse@cnrs.fr

📚Enseignement : Université Lyon I
✉️ patrice.gouet@ibcp.fr

Un poste de Maître de Conférence est ouvert pour rejoindre notre unité à Lyon !

Possibilité de rejoindre mon équipe pour travailler sur:
- caractérisation des mécanismes de défense anti-phage
- transfert horizontal et interactions entre éléments génétiques mobiles

N'hésitez pas à me contacter

Great new story from Sophie Helaine and Molly Sargen!

www.helainelab.com

PlasAnn: a curated plasmid-specific database and annotation pipeline for standardized gene and function analysis Abstract. Conjugative plasmids are key drivers of bacterial adaptation, enabling the horizontal transfer of accessory genes within and across diverse micro

Big week: welcomed a new baby boy Harris Lopatkin, AND our PlasAnn paper is finally out: academic.oup.com/nar/article/... (obviously the first more important than the second 🥰). Currently on leave but if anyone has the need to annotate large plasmids, go check it out!

New paper out in @pnas.org, and it made the cover! 👁️

We represent plasmids as circles and mutations as dots, resembling an eye, because in this paper we literally 𝑤𝑎𝑡𝑐ℎ plasmids evolve.

‼️Check Paula’s 🧵 and the paper👇

𝗣𝗹𝗮𝘀𝗺𝗶𝗱 𝗺𝘂𝘁𝗮𝘁𝗶𝗼𝗻 𝗿𝗮𝘁𝗲𝘀 𝘀𝗰𝗮𝗹𝗲 𝘄𝗶𝘁𝗵 𝗰𝗼𝗽𝘆 𝗻𝘂𝗺𝗯𝗲𝗿
www.pnas.org/doi/10.1073/...

New publication from the Lesterlin (TacC) team at MMSB! Congratulations to all authors. In collaboration with Pierre Bogaerts (UCL Belgium) and J. Villa (UB Barcelona)

doi.org/10.1093/nar/...

#microsky
Massive update of preprint with @polardlab.bsky.social!
Bacteria have evolved two systems to recombine extracellular DNA
www.biorxiv.org/content/10.1...
Kudos to lead authors Léo Hardy, Violette Morales and Clothilde Rousseau, and to outstanding Dalia's lab and @epcrocha.bsky.social
🧵⬇️

🚨L'ENS de Lyon recrute un.e maître de conférence qui rejoindra le CIRI pour développer ses recherches sur l'immunité bactérienne, les transferts horizontaux et les MGE.

📖 Une très belle opportunité d'enseigner à des étudiants brillants tout en menant des recherches excitantes !

Bacteria chromosomes contain Genomic Islands that provide virulence, antibiotic resistance, MGE-defence,... They transfer between cells, but the mechanism of most remains elusive.

Here we explore the conjugative capacity of these mysterious Genomic Islands.

www.biorxiv.org/content/10.6...

My team at @cbitoulouse.bsky.social is recruiting a postdoc #bioinformatics with solid experience in metagenomic analyses.
Interest in evolution, ecology & MGEs is important.
The offer stands until the perfect candidate is found, and it could be you 🫵

🔁 🙏

#microSky #phagesky #UTIsky
@cnrs.fr

Neisseria gonorrhoeae carries an unusually large number of restriction–modification systems (up to 16!), many of which are phase-variable. How does this affect plasmid transmission in gonococci? doi.org/10.64898/202...

Une opportunité unique de former des étudiants talentueux à la biologie des bactéries à l'ENS Lyon et mener des recherches sur les phages, l'immunité bactérienne, le transfert génétique horizontal (HGT) et/ou les éléments génétiques mobiles au sein du département de bactériologie du CIRI.

📢 Recruitment of an Associate Professor (Maître de conférences) in Bacteriology at CIRI and ENS de Lyon.
Research at @ciri-lyon.bsky.social @francoisrousset.bsky.social
@labxc.bsky.social
Anti-phage Immunity, Horizontal gene/ MGE transfer
📚 Teaching at ENS de Lyon within the Department of Biology.

Coupling DNA processing to early gene expression drives antibiotic resistance plasmid dissemination The rapid global spread of antibiotic resistance is mediated by conjugative plasmids, yet how these elements establish immediately after entering new bacterial hosts remains poorly understood. Here we...

New bioRxiv preprint from our Lesterlin lab
How early gene expression coupled to DNA processing boosts resistance plasmid spread.
With @nfrk92.bsky.social in collab with Y. Yamaichi lab
👉 doi.org/10.64898/202...

Bacterial conjugation can restructure biofilms and increase their resilience while constraining host cell dispersal Winans et al. show how plasmid transfer can reorganize bacterial communities into dense aggregates, conferring antibiotic and phage tolerance to otherwise sensitive host cells. Conjugation-dependent a...

Beautiful work from Jay Winans in the Nadell Lab @dartmouthartsci.bsky.social showing IncF plasmid pED208 spreading like wildfire through E. coli biofilms www.cell.com/current-biol...

Streptococcus pneumoniae S protein activates PBP1a to regulate peptidoglycan remodelling and cell division Nature Microbiology - S protein interacts with and activates PBP1a during cell division, as part of a larger GpsB-associated multiprotein complex that coordinates peptidoglycan remodelling in...

New publication from the Grangeasse's team at MMSB! Congratulations to all authors. In collaboration with Cecile Morlot (IBS, Grenoble) and David Rooper (Warwick university).
To know more : rdcu.be/eVsin

Pili are essential for conjugation also in many Gram-positive bacteria Type IV secretion systems (T4SS) enable the spread of antibiotic resistance and other virulence factors. In Gram-positive bacteria, T4SSs have long been thought to lack VirB2-like proteins that form c...

📣New preprint with @rberntsson.bsky.social👇We overturn the long-standing view that Gram-positive bacteria lack conjugative pili! We identify pili-forming proteins in a wide range of bacteria and show they're essential for the spread of #antibioticresistance #AMR 🧵1/6
www.biorxiv.org/content/10.6...

Poly-immunity arrays associated with Rhs toxins confer wide protection against competitors Martinkus et al. show that Photorhabdus and Xenorhabdus species encode arrays of immunity genes whose protein products neutralize toxins secreted by competitors. These arrays, often linked to Xer pass...

We propose immuno-centric view of secreted polymorphic toxin diversification and involvement of novel XPC systems in this process! Fantastic work of @jmartinkus.bsky.social with lab of @cascaleslab.bsky.social !
www.cell.com/current-biol...

Plasmid streamlining drives the extinction of antibiotic resistance plasmids under selection for horizontal transmission Conjugative plasmids play an important role in the spread of antimicrobial resistance (AMR) genes among pathogenic bacteria. This study shows that AMR loss in evolving populations is associated with t...

Our story on plasmid streamlining is now published in PLoS Biology! With @andrewmatthews.bsky.social and @sonjalehtinen.bsky.social
#MicroSky #Mevosky
journals.plos.org/plosbiology/...

Beyond RNA modification: a novel role for tRNA modifying enzyme in oxidative stress response and metabolism Abstract. RNA modifications play a fundamental role in regulating essential cellular processes, including translation fidelity and stress adaptation. While

Our new paper on DusB’s role in redox metabolism in V. cholerae is now out in @narjournal.bsky.social linking tRNAmodif enzymes and metabolic adaptation.

Beyond RNA modification: a tRNA-modifying enzyme shaping oxidative stress resilience and metabolism in V. cholerae #rnasky #rnabiology

😊🦠💫

Selective elimination of donor bacteria enables global profiling of plasmid gene expression at early stages of conjugation Abstract. Conjugative plasmids are a major driving force for the dissemination of antimicrobial resistance. During conjugation, plasmid DNA is transferred

academic.oup.com/nar/article/... Collaboration with Y. Yamaichi. Killing donor bacteria in conjugation mixes using water enables transcriptomic profiling of early plasmid genes ! Superb tool for studying zygotic induction of these early genes, which include anti-SOS and anti-RM factors. #microsky

The tRNA epitranscriptomic landscape and RNA modification enzymes in Vibrio cholerae Author summary This study charts the first genome-wide map of transfer RNA (tRNA) modifications in the cholera pathogen, Vibrio cholerae, revealing how chemical marks on tRNAs shape translation and st...

Our new paper maps the tRNA modification landscape in Vibrio cholerae! 💫
We describe differences from E. coli and discuss links to decoding of stress-related codons 🦠
Huge thanks to amazing co-authors and collaborators!
@plos.org #rnasky #microsky #tRNAmodifications

A tripartite protein complex promotes DNA transport during natural transformation in Firmicutes | PNAS Natural genetic transformation is a conserved mechanism of bacterial horizontal gene transfer, which is directed entirely by the recipient cell and...

Pleased to share our recent article in PNAS - a collaboration with @jessicaandreani.bsky.social & Pablo Radicella, with important roles played by many members of each team.

A tripartite protein complex promotes DNA transport during natural transformation in Firmicutes www.pnas.org/doi/10.1073/...

What is the best strategy to win any contest?

Eliminate your opponents of course.

Recently, my friend @fernpizza.bsky.social showed how plasmids compete intracellularly (check out his paper published in Science today!). With @baym.lol, we now know they can fight.

www.biorxiv.org/content/10.1...

Intracellular competition shapes plasmid population dynamics From populations of multicellular organisms to selfish genetic elements, conflicts between levels of biological organization are central to evolution. Plasmids are extrachromosomal, self-replicating g...

Hot off the press! Our latest paper led by @fernpizza.bsky.social, understanding how plasmids evolve inside cells. These small, self-replicating DNA circles live inside bacteria and carry antibiotic resistance genes, but also compete with one another to replicate. 1/
www.science.org/doi/10.1126/...

PhD studentship opportunity! Join us at St Andrews to study the factors controlling plasmid transmission in the gut. Competition-funded as part of the EASTBIO DTP, co-supervised with Dr Jaclyn Pearson. Please share & pass on to anyone interested! 🦠🧫 Deadline 15th December👇

Happy to share our latest NAR paper on Rel toxins targeting M. tuberculosis anti-SD region, with Tim Blower’s team (@durham.ac.uk @nebiolabs.bsky.social) and Laurent Falquet
Thanks to FRM @frm-officiel.bsky.social and CNRS @cnrsbiologie.bsky.social
academic.oup.com/nar/article-...

Specialized shuttle proteins recognize Type IX secretion signals and target effectors to their final destinations in Flavobacterium johnsoniae Communications Biology - Bacteroidota use the Type IX secretion system to secrete proteins with a conserved C-terminal domain (CTD) secretion signal domain. Type B CTDs require specific shuttle...

Happy to share the last paper form the lab:
Specialized shuttle proteins recognize
T9SS signals and target
effectors to their final destinations. Great work by @maellepllt.bsky.social, led by @thicoz.bsky.social in collaboration with @audebertstephane.bsky.social

www.nature.com/articles/s42...

What if even the core of bacterial nanomachines wasn’t static?

We found that the T3SS core protein SctD in Yersinia dynamically exchanges subunits — and this flexibility is essential for proper assembly & function.

🔗 www.nature.com/articles/s41...

@kit.edu @t3sss.bsky.social

Everything you need to explain antimicrobial resistance to family and friends, also in Spanish-5
@pablolaborda.bsky.social

How we can fight AMR

imili.org/new_gallerie...

@sbigot.bsky.social