Kenneth Ehses

Figure 1 from the preprint

Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️

www.biorxiv.org/content/10.1...
#teamtomo

What happens when the cell's powerhouse gets stressed out? 💪🥵 Great visualization in this #Mitochondria #TeamTomo study! Congrats @denkenny.bsky.social @ruferbus.bsky.social & crew 🧪 🧶🧬 🔬

Thanks Max!!!

✨Excited that the main project of my PhD is now available as a pre-print on #bioRxiv

Here, we used #CryoET to visualise mitochondrial proteostatic stress and together with SPA #CryoEM shed light into the functional cycle of the Hsp60:10 chaperone system. #TeamTomo

🔗 www.biorxiv.org/content/10.1...

🚀 My first Cryo-EM structures are OUT on the PDB!

🎉Super excited about this milestone as PhD, it’s been a journey, and I’m grateful to finally have these as my first Cryo-EM structures! 🙌

🔁Thank you for sharing!❤️

Read @daaninthelab.bsky.social et. al.: www.science.org/doi/10.1126/...

⬇️ 2nd video

Assistant/Associate Professor in Structural Biology, Biophysics or Biological Imaging

www.biozentrum.unibas.ch/open-positio...

#biozentrum #University #Basel #Professor #Structural #Biology #Biophysics #Biological #Imaging

Check out our new preprint on an integrated pipeline combining in situ #cryo-ET with MALDI #MSImaging for single-cell identification and classification from previously analysed EM-grids.
Link: www.biorxiv.org/content/10.1...

A molecular-resolution look into the near-native architecture of the spinach chloroplast🌱. This one was a long time in the oven, but we're happy to finally share our "version of record". What long-standing debates did we settle? Check back for a short thread🧵 on Monday. #TeamTomo #PlantScience 🧪🧶🧬🔬🌾

The small GTPase Ran defines nuclear pore complex asymmetry Nuclear pore complexes (NPCs) bridge across the nuclear envelope and mediate nucleocytoplasmic exchange. They consist of hundreds of nucleoporin build…

My PhD paper is in press @cellpress.bsky.social and now online: www.sciencedirect.com/science/arti...!

Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more!

Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo

Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug Nature Structural & Molecular Biology - Integrated structural biology approach leveraging in situ cryo-electron tomography reveals molecular details of the native malarial translation...

Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH

A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo

Repost appreciated:
Open #PhDposition in structural virology in my group! Join us in Umeå, Sweden to uncover how arboviruses remodel infected cells. In situ #cryoET and #cryoEM combined with #virology, #cellbiology, and #biophysics.
Deadline 7 September. More info: www.carlsonlab.se/join/

Check out 🧐 our new preprint 📜:
www.biorxiv.org/content/10.1...
Congrats🎉 to @vasilgaisin.bsky.social and Corina Hadjicharalambous, who studied contractile injection systems directly in hot spring♨️ bacterial mats🦠
#microsky #teamtomo #Chloroflexota

We are excited to announce the first in-cell structure of a LINC complex within a native nuclear envelope at subnanometer resolution!! #In-cell cryo-ET + #atomistic MD simulations! @tomdendooven.bsky.social et al!!
www.biorxiv.org/content/10.1...

Congratulations @kev-in-situ-et.bsky.social and the whole team on in situ cryo-ET images of OA-induced mitochondria damage and Parkin-dependent mitophagy out now tinyurl.com/25b82cp4. We couldn't have done it without in situ guru @wilflinglab.bsky.social and mito guru @samlewis.bsky.social .‬

Very excited to announce that my first, first author paper, is now on BioRxiv! In this paper we unambiguously show #COPII coated vesicles in unperturbed human cells for the very first time!!

Make my day and check it out!
www.biorxiv.org/content/10.1...

#Cryo-ET #TeamTomo #Cryo-CLEM ❄️🔬❄️🔬❄️🔬❄️🔬

New preprint: In situ Architecture of #Plasmodesmata.

Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores.

🔗 doi.org/10.1101/2025...

🧪🧵1/n
#teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM

Proud to share our latest paper. doi.org/10.1016/j.cr...

Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue.

Structural biology in tissue is well and truly here!

@rosfrankinst.bsky.social

BioEmu now published in @science.org !!

What is BioEmu? Check out this video:
youtu.be/LStKhWcL0VE?...

CryoET tomogram with the gap junction (green), ribosomes (cyan) and microtubules (pink)

In our latest preprint on bioRxiv, we present the in situ structure of
the human gap junction at 14 Å resolution using cryo-ET.

The structure provides a blueprint for investigating how connexin
regulation shapes intercellular communication in health and disease.

Cryo-EM structure of a natural RNA nanocage - Nature Nature - Cryo-EM structure of a natural RNA nanocage

The Fang Lab and @korostelevlab.bsky.social‬
present cryo-EM structures of two natural RNA nanocages found in bacterial and phage genomes. These findings could inform design of engineered RNA nanocages as delivery vehicles for research and therapeutic applications.

Latest preprint from the lab, many years in the making!

By combining #cryoEM with #AlphaFold3 modelling, we propose that norovirus NS3 forms a transmembrane RNA translocase.

This could have big implications for our understanding of viral replication & assembly (🧵)

www.biorxiv.org/content/10.1...

We’ve uncovered Asgard chromatin structures formed by a Hodarchaeal histone : closed hypernucleosome conserved in archaea and an open form resembling the H3-H4 eukaryotic octasome. Fantastic work by @harshranawat.bsky.social!
www.biorxiv.org/content/10.1...
#Chromatin #Asgard #Archaea #cryoEM

Very nice and generous summary of our recent preprint by @ewanbirney.bsky.social #cryoem #cryoet #proteinfolding #translocation

Molecular basis of influenza ribonucleoprotein complex assembly and processive RNA synthesis Influenza viruses replicate and transcribe their genome in the context of a conserved ribonucleoprotein (RNP) complex. By integrating cryo–electron microscopy single-particle analysis and cryo–electro...

Excited to share our new @science.org paper! Led by postdocs Ruchao Peng and Xin Xu, we used cryo-EM/ET to reveal the influenza ribonucleoprotein complex structure and its strand-sliding mechanism for RNA synthesis, paving the way for new antivirals.
www.science.org/doi/10.1126/...

A new paper from my new colleagues in Lausanne! Microsecond time-resolved cryo-EM rumbles closer day by day. Very cool work!

You want to start tomography? Solve structures inside cells? Reach Nyquist 😳 ? @phaips.vd.st and I have a website for you! tomoguide.github.io
You'll find a tutorial on how to reconstruct tomograms, pick particles and do subtomogram averaging, using different software!
Hope it will be useful !

🦠🧠 MemBrain update! 🧠🦠
We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics.
🔗 Preprint: doi.org/10.1101/2024...
🔗 Code: github.com/CellArchLab/...
🧵(1/6) #TeamTomo

Figure 4. MemBrain v2 end-to-end workflow detects periodic phycobilisome organization. A: Raw tomogram slice of EMD-31244. B: Out-of-the-box MemBrain-seg segmentation (light blue). C: A single membrane instance can be visualized in Surforama and manually annotated with GT phycobilisome positions (magenta). D: MemBrain-pick localizes particles (trained with data from C) on all membranes in the tomogram. E: MemBrain-stats computes Ripley’s O statistic using the positions from D with a bin size of 5nm. The distance between peaks (35 nm) was measured to estimate chain unit spacings.

We have updated our #MemBrain v2 preprint with a lot more details about the MemBrain-pick and MemBrain-stats modules, as well as some application examples!

Stay tuned for the upcoming thread by lead author @lorenzlamm.bsky.social! 🧠🧵

#CryoET #TeamTomo
www.biorxiv.org/content/10.1...

I'm thrilled to share that our story is now out in Science Advances! 🎉 We use quantitative imaging to map the mito central dogma, define translation hubs in the mitochondrial matrix, and show that they're replaced by Mitochondrial Stress Bodies (MSB) when mtRNA processing is perturbed 1/4

(1/3) Together with the labs of Valerie Doye @vdoye.bsky.social and Hans-Georg-Kräusslich, we analyzed NPC structure in Nup133 null mESCs. We found heterogenous NPCs featuring non-canonical symmetries and missing subunits. Now online @naturecellbiology.bsky.social.
www.nature.com/articles/s41...