Wildtype One

Why colds are personal—finally explained with a really satisfying mechanism!

Differential epithelial innate signaling (not viral load alone) driving outcomes makes the case for targeting host pathways like NLRP1 to stop severe responses

Congrats, @julienamat1.bsky.social and thanks for sharing.

This makes ‘sleep on it’ feel very literal 👀

Really elegant work—it’s the structure of replay, not its frequency, that predicts map stabilization

Also linking impaired rest to inflexible behavior makes the circuit-level deficit feel actionable

Congrats @shipleysj.bsky.social & thanks for sharing

When the genetic code gets creative 🎨🧠

It's a really clean look at how mistranslating tRNAs propagate through the proteome and phosphoproteome rather than just causing isolated errors

Congrats, @matthewberg22.bsky.social and thanks for sharing.

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As always: Wildtype One is not affiliated with these tools. This is an objective assessment based on researchers' experience and feedback 🥼 ✊

— Wildtype One 🧬

(9/9)

Pick CellEngine (CellCarta) if your main goal is speed, automation, and repeatable high-throughput pipelines with API integration, while accepting a smaller academic user base and the need to confirm that exports and audit trails fully meet publication requirements.

(8/9)

Pick Kaluza (Beckman Coulter Life Sciences) if your lab is already embedded in the Beckman ecosystem and wants a stable desktop tool for routine multicolor analysis, while accepting limited innovation in automation and weaker support for large, cloud-based collaborations.

(7/9)

Pick FCS Express (De Novo Software) if you need reliable classic gating plus strong reporting and flexible licensing for shared or offline use, but are comfortable with a feature-dense interface and potential friction from dongle or shared-license workflows.

(6/9)

Pick OMIQ (OMIQ, Inc.) if you want a modern cloud platform with clear academic pricing, fast collaboration, and Prism-friendly outputs, while accepting that it is less universally recognized by reviewers and depends on institutional cloud and data-governance rules.

(5/9)

Pick Cytobank (Beckman Coulter Life Sciences) if you prioritize cloud collaboration, shared data management, and integrated high-dimensional analysis across big studies, but are willing to invest time in strict metadata organization and pay more than for classic desktop tools.

(4/9)

Pick Cytolution (Cytolytics GmbH) if you run high-dimensional panels on large cohorts and want automated, reproducible analysis that scales well and reduces analyst bias, while accepting a smaller user community and the need to carefully validate automated results against known biology.

(3/9)

Pick FlowJo (BD Biosciences) if your lab mainly does classic manual gating and needs maximum reviewer familiarity and shared lab knowledge, but accept that it is relatively expensive and less efficient for large, automated, or highly collaborative studies.

(2/9)

FlowJo should not be the default! 🙅‍♂️

How can you choose the best flow cytometry software for your case?

Here's your rule of thumb:

(1/9)

Researchers, don't choose the default just because everyone uses it. Here's how to pick the ideal flow software for your case (see today's post on LinkedIn 👇)

www.linkedin.com/posts/wildty...

Quantitative imaging without genetic tags is huge. Super-resolution imaging being truly quantitative rather than just comparative seems more doable.

Thanks for sharing @sulianamanley.bsky.social.

Quantitative imaging without genetic tags? Huge

A nice step toward making super-resolution imaging truly quantitative rather than just comparative.

Congrats @lycasworks.bsky.social and thanks for sharing.

🧫 Join 700+ researchers getting weekly lab hacks and productivity tools (it’s free) 👉 wildtypeone.substack.com/about

Remember: If your band disappears with a new lot, it was never evidence.

— Wildtype One 🧬

(3/3)

Same catalog number

Different epitope affinity

Different background

Different story

🛠️ Low-awareness fix:

• Validate each new lot
• Run KO/KD control once, not forever
• Never trust a single antibody

(2/3)

Researchers,

If your blot only works in your hands, it’s not evidence yet. The silent killer? Antibody lot change

(1/3)

Ignore these 10 reviewer comments “What reviewers should be doing is judging whether the conclusions follow from the premises—not whether they would have done the work differently.” — Sydney Brenner, Nobel Prize Laureate Dear Research...

Remember: Peer review is a human process, and some comments can harm your work 🔬

Wildtype One looked at true stories from researchers

We found 10 reviewer comments you should absolutely ignore

Read today's Wildtype Weekly to find them out 👇

It was likely used because researchers wanted to go home and sleep rather than wait for the antibody!

You can get results that are as good (or even better) if you incubate 4 hours at room temperature

Sometimes 3h is enough

You'll gain a day

...and finish your experiment faster

— Wildtype One 🧬

Quick tip 🔬🧑‍🔬

If you do Western blots, immunofluorescence, or any technique that requires antibody staining

Remember:

Overnight incubation at 4°C is not always the best protocol ⚠️

(1/2)

Thanks for spreading the wors @uniofnomi.bsky.social 🙌🏻

Evolutionary physiology eating well this year!

Congratulations on the publication, @seiliez.bsky.social. A nice mechanistic link between central CMA activity and feeding behavior

It adds an unexpected but compelling layer to how satiety and metabolic regulation are controlled

A compelling mechanistic bridge between EBV exposure, MS genetics, and pathogenic T cell responses

The convergence of cross-reactive CD4 clonotypes, HLA restriction, and inflammatory cytokine output makes the mimicry model feel unusually concrete here

Thanks for sharing @jackamatica.bsky.social

Linking impaired presynaptic LTP and disrupted local translation at mossy fibers makes a strong case that spatial control of protein synthesis is essential for long-term plasticity

Congrats on the paper and the elegant demonstration with subcellular mRNA localization, @npanayotis.bsky.social

All 8 Western blot failures and how to prevent them (2026 Guide) Western blotting is a defeating experiment. Unlike Flow cytometry failures, Western blot issues need a bit more head scratching.

Here's a full catalog of horror stories:

"Read this if you're having a bad day in the lab" kinda story

@ishier.bsky.social @lucygdornan.bsky.social

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