Nimesh Chahare

Congratulations Isabela 🎉 🎉

Happy to share that my PhD has finally taken the shape of a paper! ✨

Huge thanks to all authors, especially @davidbrueckner.bsky.social & @ehannezo.bsky.social for leading the modeling and @raimonsunyer.bsky.social & @xaviertrepat.bsky.social for their invaluable supervision.

🧵👇

Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells - Nature Cell Biology McCreery, Stubb et al. show that mechano-osmotic changes in the nucleus induce general transcriptional repression and prime chromatin for cell fate transitions by relieving repression of specific differentiation genes.

How do #stemcells integrate information to coordinate fate decisions? Delighted to finally see our work showing how growth factors regulate the mechano-osmotic state of the #nucleus and #chromatin to control #pluripotency exit out! www.nature.com/articles/s41... see 🧵 👇

Gut epithelium modifies enteric behaviors during nutritional adversity via distinct peptidergic signaling axes Gut epithelium modulates output from distinct enteric circuits by altering secretion of insulin and noninsulin peptides.

Very excited to share this finding from my postdoctoral work that is now published in #ScienceAdvances. We show how the gut’s epithelium modifies enteric behaviors during nutritional adversity via distinct peptidergic signaling axes.

www.science.org/doi/10.1126/...

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Mechanosensitive and Reversible Chromatin-Lamina Dewetting Triggers Cellular Contraction During Wound Healing Tissue wounding causes rapid mechanical changes in the epithelium, but how these changes affect intracellular organization remains unclear. Using the Drosophila embryonic epidermis, we show that wound...

1/ Link: www.biorxiv.org/content/10.1...
✂️ Wounding in the fly epidermis makes chromatin compact + detach from the lamina.
⏱️ Minutes later it re-expands & re-attaches.
A reversible, nuclear “dewetting” cycle right at the wound edge.

👀🚨 Fantastic preprint from @andreuion.bsky.social

There's a lot going on inside the nucleus. #EpithelialMechanics

We know tissue mechanics matter in healing, but what happens at the chromatin level?
Our new preprint shows that chromatin briefly “de-wets” from the lamina, then re-wets to kick off repair.
Mechanics→chromatin→contraction→healing.
Led by amazing PhD students Guillermo Bengoetxea & Lenka Backová 🙌

If you are interested in cell compression in general (not only in epithelial systems) in how to control it in vitro, in the associated mechanotransduction mechanisms and on their consequences, you can check our latest review!
bsky.app/profile/epim...

We believe that the combination of various in vitro tools, can complement in vivo observations. By doing so, Conti et al. showed that stem vs differentiated cancer cells present different mechano-phenotypes, suggesting a differential metastatic potential.
bsky.app/profile/epim...

Ever wondered how to control stretch/compression in your epithelial system? Do you know why it’s important, which mechanotransduction mechanisms could be involved? I am @valeriaventurini.bsky.social and today I will guide you through this journey.

Incredible!!! 😍

It allows shaping the electric field, from single cells (first video) to sculpting whole tissues (movie below). This was several years of Yubin and Jeremy's lives; it's now changing what we know and do with electricity to control cell behaviors. It's so cool--please use it, we will help/share!

SCHEPHERD--the bioelectric cell herding platform built for YOU. Single cells, monolayers, organoids--this herds them all + new tricks. Plz try it-- we will *give* you parts! Teaser here of a steering a single cell. GS Yubin Lin's lifeblood with J. Yodh on piano; Celeste R. and Paul K. Thread 1/N

Tissues transition between solid & fluid states in development & disease; cytoplasm turns crowded or inert to literally 'solidify'. Any connections? Turns out nothing's known. @sameerthukral.bsky.social & @bipashadey29.bsky.social pulled this review off brilliantly with a conceptual synthesis.

How can we use cell shapes and geometry to study mechanics of tissues?

I'm @juliaeckert.bsky.social, and with this thread, I would like to show you the secrets of cells and tissues. Let’s connect biology with a little bit of physics. Don't be scared and get inspired 😀. #EpithelialMechanics

📣 New preprint! We present SpinePy, a modular framework that enables automated 3D spatiotemporal quantification of #gastruloids & other multicellular in vitro systems. Spearheaded by @cryaaa.bsky.social @mpi-cbg.de. Check out his thread 👇 & the paper:
www.biorxiv.org/content/10.1...

The actin cortex acts as a mechanical memory of morphology in confined migrating cells - Nature Physics Cells often navigate through confined spaces. Now it is shown that cells retain a mechanical memory of previous confinement events, which makes them more efficient at migrating through narrow microenv...

🤔 Can migrating cells 'remember' their past trajectories?

In collab w/ @sgabriele.bsky.social & @kyohalie.bsky.social, we address this question:

Confined cells undergo geometry-sensitive morphology switches, and these switches depend on the past migration history!

www.nature.com/articles/s41...

Compression causes cancer cells to start spreading The switch between proliferation and invasion is triggered by mechanical force.

new out in Nature www.nature.com/articles/d41...

Super excited that our group will be supported by an ERC Starting Grant!

In project "InfoFate" we will study how cells use information in dynamical, neighborhood & mechanical signals to make decisions.

We'll have PhD and Postdoc positions available, please get in touch if interested!

The last paper from my PhD is now online! 🥳

👉 pubs.aip.org/aip/rsi/arti...

We have designed a new device based on the elastic micropillar array assay to measure the detachment forces between spread cells.

@leidenphysics.bsky.social

Congratulations Tom!!! 😍🎉🎉

Check out the preprint of my main work in the lab of @xaviertrepat.bsky.social @IBECBarcelona. Many thanks to all the people involved!

New preprint! 🚨 We uncover a slow adaptation to stretch that links star-bundling of keratin filaments with nuclear escape from its keratin cage. Led by @tomgolde.bsky.social‬ 🙌
@IBECBarcelona
www.biorxiv.org/content/10.1...

🚨wonderful thread by @benswedlund.bsky.social!
Tissue engineering via self assembly 👀

Are different cell types like oil and water? Over a century ago, researchers found that dissociated sponges and embryonic tissues can self-assemble into functional structures. Join me, @benswedlund.bsky.social to explore the differential adhesion hypothesis & its applications in tissue engineering!

Collective cell motion has many forms, but rotation is the coolest of them all.

I'm @onenimesa.bsky.social , and in this short🧵, I'll highlight some instances of global tissue rotation like this one from @BauschLab

🚨 New paper out in @pnas.org! 🚨

We show that self-generated gradients allow heterogeneous cell mixtures to co-migrate efficiently over long distances —while optimizing their physical interactions!

(See below for 🧵)

www.pnas.org/doi/10.1073/... 🧪

Congratulations 🎉 🎉
very useful tool for #epithelialmechanics

Now published in @natcomms.nature.com! 🥳

👉 rdcu.be/eATn3

We developed image analysis tools to capture the nematic orientation field of 3D tissue surfaces. Tested on epithelial aggregates, zebrafish hearts, myoblasts on spheres & micro-vessels, we combined soft matter physics with exp. biology.

Synthetic materials accumulate damage with every mechanical challenge. Epithelial tissues are regularly loaded, yet healthy epithelia rarely rupture. Why?

We are @epmech.bsky.social and @baldaufsci.bsky.social and in this thread we introduce you to cyclic loading.

bsky.app/profile/epim...