Bryjalab

MILESTONE International Conference | MILESTONE International Conference CK1 | MUNI SCI CK1 protein kinase family: From cell signaling to therapeutic targets in human disease

🎉 We’re thrilled to announce that the MILESTONE International CK1 Conference is happening July 21–23, 2025, in Brno, CZ! 🫛 Join us to explore CK1 kinases in cell signaling, cancer, neurodegeneration, therapy & more!
Registration’s open now! Looking forward to meeting you all there! 🤗 ck1.sci.muni.cz

Mechanisms of transcription attenuation and condensation of RNA polymerase II by RECQ5 The elongation rates of RNA polymerase II (RNAPII) require precise control to prevent transcriptional stress, which can impede co-transcriptional pre-mRNA processing and contribute to many age- or dis...

🍾 New preprint alert! We were happy to methodologically participate in the project that targets the regulation of RNA Polymerase II transcription by RECQ5-mediated LLPS. 🧊🔬 Huge thanks to the Richard Štefl Lab and @labvacha.bsky.social for this opportunity! 🤗
www.biorxiv.org/content/10.1...

🤗 A huge thanks to the Czech Science Foundation and the National Infrastructure for Chemical Biology for supporting our work on CK1 δ/ε degraders! Stay tuned for more CK1 biology updates! 👩‍🔬

📌AH078 is a powerful tool for studying CK1 δ/ε biology in cells, enabling rapid depletion of both kinases—something challenging with KO systems due to cellular dependency on CK1 δ/ε activity!

👉 The PROTAC AH078 is characterized through in vitro and cellular assays, demonstrating on-target activity, selectivity, and a VHL-dependent mechanism of the CK1 degradation in cells.

Development and Discovery of a Selective Degrader of Casein Kinases 1 δ/ε Members of the casein kinase 1 (CK1) family have emerged as key regulators of cellular signaling and as potential drug targets. Functional annotation of the 7 human isoforms would benefit from isoform...

🥳New paper from the lab!🍾 Excited to share our work with the Stefan Knapp lab (Goethe Uni, @thesgc.bsky.social & DKTK), published in J. Med. Chem. @pubs.acs.org. Here, we present the first degraders (PROTACs) targeting CK1 δ/ε—highly selective across the kinome & within the Casein kinase 1 family. 🧵

📢 Greetings from Dundee! Our PhD student Tomáš Gybeľ sends his best from his internship in the Sapkota lab at the University of Dundee, where he is studying CK1 and the Fam83 protein family in Wnt signaling. 🌍🔬 Big thanks to Prof. Sapkota and his team for their warm welcome and support! 🤗

Bryjalab attended the 25th Prague Hematology Days 🩸, presenting our work on ROR1+ B cells in CLL, CK1 inhibitors in AML, and the effect of CK1 inhibition + Venetoclax on specific AML clones. 👩‍⚕️👩‍🔬 Huge thanks to the organizers for the chance to share our research with scientists and clinicians! 🤗

Our PhD student @petrakompanikova.bsky.social is helping organize this amazing conference with a really impressive lineup of invited speakers. Follow her and @v4sdb.bsky.social for updates, and hope to see you all in the High Tatras!

Today, during the Open Day at the Faculty of Science, Masaryk University, I had the chance to help my department showcase our research and guide prospective bachelor students through our labs. 🔬🧪 I hope we sparked some interest and inspired a future scientist! ✨👩‍🔬👨‍🔬

Postdoc in Cancer Biology Open postdoctoral position at the Institute of Biophysics of the Czech Academy of Sciences in Brno, Czech Republic The research group, headed by Dr. Karel Souček at the Institute of Biophysics of t…

We are #hiring!
#Postdoc in #cancerBiology
Read more: researchjobs.cz/VwOxh
#postdoctoral #jobs #Brno #cellBiology #molecularBiology #biochemistry #postdocjobs

Lastly, we would like to thank the Czech Science Foundation, National institute for cancer research,
@ciisbinstructcz.bsky.social and e-infra.cz for their support and resources. 🤗
Stay tuned for more updates! 🐟🔬✨
#Wnt #PCP #CellBiology #DevelopmentalBiology #CancerResearch #NGEU

This work wouldn’t have been possible without our excellent team—especially our incredible co-shared first authors,
@kgomory.bsky.social, Nikodém Zezula and Tomasz Radaszkiewicz

Why does this matter?
The Wnt/PCP pathway plays a critical role in developmental biology, tissue organization, and the hallmarks of cancer. Dysfunctions in this pathway can result in severe developmental defects or contribute to cancer progression.

This study not only uncovers novel components of the Wnt/PCP pathway but also provides a comprehensive resource for further exploration of Wnt/PCP function in vertebrates. 🌟

Further, we analyzed these proteins in melanoma cell lines. Loss of the endogenous level of their expression lead to the inhibition of the cancer cells migration, further validating our results.

Among the candidates, three stood out as essential:
•RAI14
•EPHA2
•PHACTR4
These proteins are key for Wnt/PCP processes like:
✅ Zebrafish convergent extension
✅ Orientation of lateral organ cells

By analyzing individual preys across baits and their subcellular locations, we identified 30 candidate proteins that might play a role in Wnt/PCP signaling. To validate these, we performed loss-of-function studies in zebrafish. 🐟

We used an advanced technique called proximity-dependent biotinylation (BioID) to map the intracellular interactome of the key Wnt/PCP components ROR1, ROR2, VANGL2, DVL3 and PRICKLE1.

Proximity interactomics identifies RAI14, EPHA2 and PHACTR4 as essential components of Wnt/planar cell polarity pathway in vertebrates Wnt/planar cell polarity (Wnt/PCP) pathway is an evolutionarily conserved signaling cascade playing an inevitable role in cell biology. Deregulation of Wnt/PCP leads to severe developmental defects or...

🚨Preprint alert🚨 We're excited to share our latest findings on the Wnt/planar cell polarity (Wnt/PCP) pathway, a critical signaling cascade for cell biology, development, and disease.
🧵👇
www.biorxiv.org/content/10.1...

🩸We explore how to target Wnt signaling by inhibition of casein kinase 1 (CK1). We develop novel CK1 tools and analyze their impact on cell migration and interactions with the tumor microenvironment particularly in leukemia and melanoma. We aim to uncover therapeutic targets with real-world impact.🤗

🐭 We investigate the Wnt/Planar Cell Polarity (PCP) pathway, crucial for cell polarity, migration, formation of branched structures, and cancer metastasis. Our tools? Advanced proteomics, microscopy, scRNAseq, PCP-deficient cell lines, zebrafish and mouse models.

🔍 Our research dives into Dishevelled (DVL) proteins, which act as a key switch in Wnt signaling. Using CRISPR, proteomics, and structural biology methods, we aim to decode how DVL phosphorylation, localization, and conformation drive cellular communication.

Hi Bluesky! 🌌 We are Bryjalab – the Laboratory of Cellular Communication 🧬 at Masaryk University, Brno, Czech Republic. From development 🐣 to disease 🩺, we explore the mechanisms and effects of Wnt signaling cascades. 🧵 Follow us for more Wnt updates! 🚀