Bryjalab

🎉 Pleased to collaborate again with Buchtová lab, where @petrakompanikova.bsky.social’s bioinformatics skills contributed to uncovering new roles of LGR5 in the formation of teeth 👩‍💻🐁

🤗 This work would not have been possible without the fantastic collaboration between our lab and K. Tripsianes’ lab at @ceitec.eu. Also, huge thanks to our funding sources — the Czech Science Foundation & the Ministry of Education, Youth and Sports. 👏

🧐In a nutshell: CK1-mediated multisite phosphorylation of Dishevelled dramatically alters its bulk electrostatics, promoting DVL intramolecular coupling between the positively charged cleft of the DEP domain and the adjacent phosphorylated disordered region.

A Wnt-induced conformational phospho-switch in DVL3 controls interaction with Frizzled receptors and Wnt/β-catenin signaling When Wnt ligands bind to Frizzled (FZD) receptors, Dishevelled protein (DVL) gets multiphosphorylated by Casein kinase 1 (CK1). Although, it is well known that DVL phosphorylation relays Wnt signals f...

Excited to share our new preprint on bioRxiv. 🎉
We uncovered a Wnt-induced phospho-switch of Dishevelled. This intramolecular coupling is mutually exclusive with FZD engagement and functionally required for the Wnt/β-catenin signaling. 🧵
doi.org/10.1101/2025...

Many thanks @chriswaudby.bsky.social , and also to @michaelranes.bsky.social and Lucie Wolf, for coming all the way to Brno and doing such an excellent job as examiners. 👏

Big day for Bryjalab yesterday! Mirek Micka & Tomáš Gybeľ nailed their defences — welcome to the PhD club! 🎓🔥 Thanks to everyone who supported them along the way and came to celebrate! 🎉🤗

Day 2 of our Milestone minisymposium at @ceitec.eu in Brno was packed with excellent talks by Lucie Wolf from Stephane Angers’ lab at @utoronto.ca, followed by @cajaneklab.bsky.social and Víťa as the closing speaker. 🔥

The science marathon continues. 🏃
Straight off #EBBM2025, BryjaLab is co-organizing with Tripsianes’ lab at @ceitec.eu the Milestone Minisymposium on Cell Signalling, kicking off today with great talks by @chriswaudby.bsky.social & @michaelranes.bsky.social. More talks & two PhD defenses tomorrow 🎓

Grind stays real – Víťa and @petrakompanikova.bsky.social went straight from the V4SDB Meeting 2025 to the European Brain Barrier Meeting in Bern, where Petra presented her work on the hindbrain choroid plexus. 🐁 Big thanks to the organizers and community for the warm first-time welcome! 😇 #EBBM2025

Big shout-out to @sumbalovakoledova.bsky.social, Marcela Buchtová, Alexandra Špirková & our PhD student @petrakompanikova.bsky.social, who also presented a poster on her Choroid Plexus development story, for organizing such an excellent meeting 🔥

Last week we joined the @v4sdb.bsky.social Meeting 2025 in the beautiful High Tatras 🌄 with an amazing lineup of speakers and posters 🔥 So good to see this society still rocking!

And huge thanks for the support @biologists.bsky.social, @ceitec.eu, @uebscimuni.bsky.social, Milestone & CasInvent Pharma 🤗🎉

First-ever CK1 Protein Kinase Family conference took place in Brno, bringing together nearly 80 researchers from around the world to share the latest findings on CK1 biology, disease links, and therapeutic potential. Thanks for the inspiring talks, posters & great networking – see you next time! 🎉🥳

MILESTONE International Conference | MILESTONE International Conference CK1 | MUNI SCI CK1 protein kinase family: From cell signaling to therapeutic targets in human disease

🎉 We’re thrilled to announce that the MILESTONE International CK1 Conference is happening July 21–23, 2025, in Brno, CZ! 🫛 Join us to explore CK1 kinases in cell signaling, cancer, neurodegeneration, therapy & more!
Registration’s open now! Looking forward to meeting you all there! 🤗 ck1.sci.muni.cz

Mechanisms of transcription attenuation and condensation of RNA polymerase II by RECQ5 The elongation rates of RNA polymerase II (RNAPII) require precise control to prevent transcriptional stress, which can impede co-transcriptional pre-mRNA processing and contribute to many age- or dis...

🍾 New preprint alert! We were happy to methodologically participate in the project that targets the regulation of RNA Polymerase II transcription by RECQ5-mediated LLPS. 🧊🔬 Huge thanks to the Richard Štefl Lab and @labvacha.bsky.social for this opportunity! 🤗
www.biorxiv.org/content/10.1...

🤗 A huge thanks to the Czech Science Foundation and the National Infrastructure for Chemical Biology for supporting our work on CK1 δ/ε degraders! Stay tuned for more CK1 biology updates! 👩‍🔬

📌AH078 is a powerful tool for studying CK1 δ/ε biology in cells, enabling rapid depletion of both kinases—something challenging with KO systems due to cellular dependency on CK1 δ/ε activity!

👉 The PROTAC AH078 is characterized through in vitro and cellular assays, demonstrating on-target activity, selectivity, and a VHL-dependent mechanism of the CK1 degradation in cells.

Development and Discovery of a Selective Degrader of Casein Kinases 1 δ/ε Members of the casein kinase 1 (CK1) family have emerged as key regulators of cellular signaling and as potential drug targets. Functional annotation of the 7 human isoforms would benefit from isoform...

🥳New paper from the lab!🍾 Excited to share our work with the Stefan Knapp lab (Goethe Uni, @thesgc.bsky.social & DKTK), published in J. Med. Chem. @pubs.acs.org. Here, we present the first degraders (PROTACs) targeting CK1 δ/ε—highly selective across the kinome & within the Casein kinase 1 family. 🧵

📢 Greetings from Dundee! Our PhD student Tomáš Gybeľ sends his best from his internship in the Sapkota lab at the University of Dundee, where he is studying CK1 and the Fam83 protein family in Wnt signaling. 🌍🔬 Big thanks to Prof. Sapkota and his team for their warm welcome and support! 🤗

Bryjalab attended the 25th Prague Hematology Days 🩸, presenting our work on ROR1+ B cells in CLL, CK1 inhibitors in AML, and the effect of CK1 inhibition + Venetoclax on specific AML clones. 👩‍⚕️👩‍🔬 Huge thanks to the organizers for the chance to share our research with scientists and clinicians! 🤗

Our PhD student @petrakompanikova.bsky.social is helping organize this amazing conference with a really impressive lineup of invited speakers. Follow her and @v4sdb.bsky.social for updates, and hope to see you all in the High Tatras!

Today, during the Open Day at the Faculty of Science, Masaryk University, I had the chance to help my department showcase our research and guide prospective bachelor students through our labs. 🔬🧪 I hope we sparked some interest and inspired a future scientist! ✨👩‍🔬👨‍🔬

Lastly, we would like to thank the Czech Science Foundation, National institute for cancer research,
@ciisbinstructcz.bsky.social and e-infra.cz for their support and resources. 🤗
Stay tuned for more updates! 🐟🔬✨
#Wnt #PCP #CellBiology #DevelopmentalBiology #CancerResearch #NGEU

This work wouldn’t have been possible without our excellent team—especially our incredible co-shared first authors,
@kgomory.bsky.social, Nikodém Zezula and Tomasz Radaszkiewicz

Why does this matter?
The Wnt/PCP pathway plays a critical role in developmental biology, tissue organization, and the hallmarks of cancer. Dysfunctions in this pathway can result in severe developmental defects or contribute to cancer progression.

This study not only uncovers novel components of the Wnt/PCP pathway but also provides a comprehensive resource for further exploration of Wnt/PCP function in vertebrates. 🌟

Further, we analyzed these proteins in melanoma cell lines. Loss of the endogenous level of their expression lead to the inhibition of the cancer cells migration, further validating our results.

Among the candidates, three stood out as essential:
•RAI14
•EPHA2
•PHACTR4
These proteins are key for Wnt/PCP processes like:
✅ Zebrafish convergent extension
✅ Orientation of lateral organ cells