Stefano Arfè

I am very grateful to the great mentors and collaborators whom I have worked with Geneviève Almouzni Jean-Pierre Quivy Eran Meshorer Tina Karagyozova, PhD David Mazaud Dominic Bingham Hatem Hmidan Patricia Le Baccon Audrey Forest and, if you have any questions, don't hesitate to drop me a line!

Why does this matter? Because chromatin disorganization is a hallmark of cancer and developmental disorders. Our findings point to a new layer of genome regulation—one that could be harnessed to keep stem cells healthy, or even to design new therapies for diseases where genome stability breaks down.

But here's the kicker: when we disturbed this balance, stem cells showed abnormal nuclear shapes, struggled during cell division, and often died. This reveals that the interplay between H3.1 and H3.3 is critical for maintaining genome stability and healthy cell division.

Using cutting-edge microscopy, genome sequencing, and engineered cell models, we found that this balance shifts dynamically during the cell cycle and varies between stem cells and differentiated cells.

We focused on two key players: H3.1, which is deposited during DNA replication, and H3.3, which is incorporated independently of replication. These histones compete for space at chromocenters, specialized nuclear compartments essential for chromosome stability.

H3.3 deposition counteracts the replication-dependent enrichment of H3.1 at chromocenters in embryonic stem cells - Nature Communications In mammals, two major non-centromeric histone H3 (H3.1/2 and H3.3) variants, display distinct nuclear distribution patterns across the genome and use distinct deposition pathways. Here the authors rev...

🔬✨ Excited to share a major milestone from my PhD research at Institut Curie, where I am a first author in a recently published paper in Nature Communications!
www.nature.com/articles/s41...

My drop contribution to the ocean.

I’ve just done my #eXit! Thanks to #HelloQuitX I've registered my previous followers to #BlueSky. Join us on app.helloquitx.com! A mai piu' #X

Ho Lab enjoyed a great #ALS Network Research Summit in San Francisco!

Institut Pasteur decides to leave X The Institut Pasteur, a research organization which for more than 130 years has been committed to tackling infectious diseases, sharing knowledge and defending science, has decided to leave X because ...

"Institut Pasteur has decided to leave X because of serious abuses observed on the platform... oppose[s] the campaign by Musk to weaken democracies and destabilize Europe's institutional foundations" www.pasteur.fr/en/home/pres...