Sagar Khavnekar

STOPGAP: an open-source package for template matching, subtomogram alignment and classification STOPGAP, an open-source package for subtomogram averaging that is designed to provide users with fine control over each of the steps in the image-processing workflow, is described.

As @cellarchlab.com said template based approaches are powerful! However we are limited to roughly 200-300 kDa and abundant targets. Have a look at journals.iucr.org/d/issues/202... to get an idea and a systematic analysis from Beata’s team www.nature.com/articles/s41...

This has been a journey with Abhay Kotecha and @cellarchlab.com
From conception to the preprint 😇🥳
And couldn’t have started it without @wojwie.bsky.social @florentwaltz.bsky.social

Towards community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii In situ cryo-electron tomography (cryo-ET) has emerged as the method of choice to investigate structures of biomolecules in their native context. However, challenges remain in the efficient production...

Hey #teamtomo checkout our #teamscience approach to #cellbio #cryoET

I can’t thank @cellarchlab.com enough for this one 😍😍

Awesome team science from @mpibiochem.bsky.social @biozentrum.bsky.social Thermo Fisher Scientific and more

#firstpost on Bluesky

www.biorxiv.org/content/10.1...