David Turner

Figure 4 WT1 is expressed in the primitive streak, emerging mesoderm, neuromesodermal progenitor cells and LPM in E8.5 mouse embryos

New insights directly link WT1 and smooth muscle cell lineages

Read this Research Highlight showcasing work from Suad Alsukari, Huei Teng Ng, @gastruloids.bsky.social, Bettina Wilm @bwilm.bsky.social and colleagues:
journals.biologists.com/dev/article/...

Dynamic WT1 expression during gastrulation specifies peritoneal smooth muscle fate independently of mesothelial fate Highlighted Article: Genetic lineage tracing and expression analyses reveal insights into the developmental lineage of smooth muscle cells in the intestine and mesentery, specified by WT1 expression d...

Very happy to announce that this article is now published @dev-journal.bsky.social @biologists.bsky.social
journals.biologists.com/dev/article/...
With Suad Alsukari and Huei Teng Ng and @gastruloids.bsky.social

An englishman, down and out in Paris…..trying to read the news and laughing....at a french joke?
@gastruloids.bsky.social‬

Huge thanks to‬ @cci-liv.bsky.social for the amazing microscopes and technical support throughout this work!

Domain-specific AI segmentation of IMPDH2 rod/ring structures in mouse embryonic stem cells - BMC Biology Background Inosine monophosphate dehydrogenase 2 (IMPDH2) is an enzyme that catalyses the rate-limiting step of guanine nucleotides. In mouse embryonic stem cells (ESCs), IMPDH2 forms large multi-prot...

Look at this amount of work that has gone into the analysis of these beautiful (yes, we are biased) images of rods and rings. Now you can automatically classify these structures which is rather tedious to do manually. 🔬📷🖼️💻📄 bmcbiol.biomedcentral.com/articles/10....

I cannot help but think of these rod-rings as oh so pretty. amazing work by some of our @cci-liv.bsky.social users.

Great preprint out from the MicroAge team in
@livuni-ilcams.bsky.social! www.biorxiv.org/content/10.1...

Excellent work supporting #modularity of the mammalian #embryo, a role for #hypoxia & how development exploits these features. It shows what we can learn from assemblies of #gastruloids @ spatio-temporal coordination of the modules in AP organization. Putting modules together, extremely informative

The modularity of the mammalian #embryo in display. Controlled assembly of classic #gastruloids and uncommitted precursors in #hypoxia, patterns the AP axis
www.biorxiv.org/content/10.1...
Beautiful & insightful from @bulutkarslioglu.bsky.social‬ group. Building models to learn and learning.

This was a fabulous collaboration! Thanks @gastruloids.bsky.social and to Suad and Huei who worked incredibly hard to make it happen!

Also, an interesting reflection from Bob Edwards on the contrast between Research Institutes and Universities. Having spent my academic life in the latter, I can relate. From "A matter of life" a first hand account of the path to IVF that I suggest as a read for those interested in human #DevBio

Dynamic WT1 expression during gastrulation specifies peritoneal smooth muscle fate independently from mesothelial fate The Wilms’ Tumour protein (WT1) was previously linked to the mesothelial and vascular smooth muscle cell (vSMC) lineage in the mouse intestine, with evidence suggesting that intestinal vSMCs arise fro...

Great to see our article published in @dev-journal.bsky.social! Wonderfull collaboration with @bwilm.bsky.social to understand how Wt1 guides cell fate in the early gastrulating embryo! journals.biologists.com/dev/article/...

A time-resolved, multi-symbol molecular recorder via sequential genome editing - Nature A DNA memory device, DNA Typewriter, uses sequential prime editing to record the order of multiple cellular events.

We previously showed that DNA Typewriter can record high-resolution lineage trees in cultured cells (HEK293T). www.nature.com/articles/s41... But could we use it to study how clonal memory shapes cell fate decisions during development? (2/13)

Lineage recording in monoclonal gastruloids reveals heritable modes of early development Mammalian stem cells possess a remarkable capacity for self-organization, a property that underlies increasingly sophisticated in vitro models of early development. However, even under carefully contr...

New preprint with the Shendure lab @jshendure.bsky.social ! We apply the DNA Typewriter lineage recorder to mouse 3D gastruloids, a powerful model of early mammalian development. Led by Sam Regalado and CX Qiu @cxqiu.bsky.social : www.biorxiv.org/content/10.1... (1/13)

We've just updated our manuscript to a more 'publishable' version!
www.biorxiv.org/content/10.1...
New details about the role of Wnt in suppressing neural differentiation and fostering a posterior epiblast state, and the temporal effect of Nodal signalling in driving mesendodermal fates

Domain-specific AI segmentation of IMPDH2 rod/ring structures in mouse embryonic stem cells - BMC Biology Background Inosine monophosphate dehydrogenase 2 (IMPDH2) is an enzyme that catalyses the rate-limiting step of guanine nucleotides. In mouse embryonic stem cells (ESCs), IMPDH2 forms large multi-prot...

Our new paper just arrived! Fun collaborative effort led by Sam Ball and Meagan Hennessy to develop an AI tool to segment and classify #IMPDH2 rod-rings in mouse ESCs doi.org/10.1186/s129...

Gala dinner of the Biologists @ 100 conference at St Georges Hall, Liverpool.

Great fun at last night's Gala Dinner at the stunning
St Georges Hall.
#biologists100

Right after the spanish edition of #TheMasterBuilder comes the Portuguese one, thanks to www.temasedebates.pt
Have a look for a cell’s eye view of Biology in contrast with the widespread gene’s eye.
#CellsRUs #NotInTheGenes

Wonderful evening in #Barcelona w/ friends sharing memories, science and hope in these troubled times.

In the wake of Barcelona #Gastruloid meeting, we can say w/ some confidence that this is a system w/ potential in several areas of biomedical research: models, rather than replicas, are useful &also teach. For details
linkinghub.elsevier.com/retrieve/pii...
onlinelibrary.wiley.com/doi/10.1002/...

Compound design of a patient-derived 3D cell culture system modelling early peritoneal endometriosis Peritoneal endometriosis causes pelvic pain and infertility, but the underlying mechanisms related to these symptoms are not fully understood. Endometriosis diagnosis is typically delayed; thus, patie...

Delighted to announce our preprint on modelling of the peritoneum in endometriosis research, by Dimas Rahmana, with Dharani Hapangama's group, Liverpool Women's Hospital, with essential contribution from Chris Hill
www.biorxiv.org/content/10.1...
@livunihls.bsky.social @liverpooluni.bsky.social

First day of our Gastruloid Meeting in Barcelona kicking off with amazing talks and discussion and a room filled with gastruloid enthusiasts! #gastruloids #conference

Fun collaboration! All the nitty-gritty about N2B27 and gastruloids. Kudos to all the authors, particularly Tina Balayo (¡que va de primero!)

N2B27 media formulations influence gastruloid development Gastruloids are 3D aggregates of pluripotent stem cells grown in suspension culture that mimic many aspects of gastrulation and early axial elongation. The N2B27 basal medium in which mouse gastruloid...

New Gastruloid preprint just landed... nice collaboration between our lab, @dias-andre.bsky.social, and @amartinezarias.bsky.social www.biorxiv.org/content/10.1...

A temporal coordination between Nodal and Wnt signalling governs the emergence of the mammalian body plan Nodal and Wnt signalling play an important role in the emergence of the mammalian body plan, primarily by orchestrating gastrulation and primitive streak formation. While the literature suggests that ...

www.biorxiv.org/content/10.1...

Here we explore the role of #Wnt and #Nodal signalling during mammalian gastrulation using #gastruloids. Building on the work of many labs, we propose that antagonistic signalling-dependent #modularity in the PS establishes the early mammalian body plan.

This is probably the future of science. But to set it up? @gastruloids.bsky.social @dwilkinsonlab.bsky.social

MRC DiMeN Doctoral Training Partnership: Understanding skeletal tissue responses to sex and menopause in osteoarthritis at University of Liverpool on FindAPhD.com PhD Project - MRC DiMeN Doctoral Training Partnership: Understanding skeletal tissue responses to sex and menopause in osteoarthritis at University of Liverpool, listed on FindAPhD.com

A remonder we have a MRC funded PhD studentship open for application. Supported by a great multidisciplinary team, with Biomedical and engineering methods to explore how sex and menopause affect osteoarthritis. Please share!
www.findaphd.com/phds/project...