David Turner

Listening to David Lammy on @BBCr4today attempting to justify abolishing trial by jury, it is increasingly and terrifyingly clear that he simply does not understand the issues.

As set out in this thread, the causes of the court backlog are chronic lack of funding and absurd inefficiency.

The government has made no proposals to address either. Their answer is to remove juries.

Rather than fix the leaking roof, their solution is to burn the house down.

Now out in @dev-journal.bsky.social
Lots of new results! Check it out!!
journals.biologists.com/dev/article/...

There's increasing evidence that in organoid/embryoids conditions of cells at start of experiments is crucial/ determines outcome. Much to be learnt still. Taking advantage of mouse #gastruloids, two groups explore this here. Important work to calibrate the field www.biorxiv.org/content/10.1...

N2B27 media formulations influence gastruloid development Highlighted Article: This work explores the cellular and molecular sensitivity of the mouse gastruloid model system to variations in N2B27 media formulations, highlighting the need for careful media s...

Now out in @dev-journal.bsky.social
journals.biologists.com/dev/article/...

N2B27 media formulations influence gastruloid development Highlighted Article: This work explores the cellular and molecular sensitivity of the mouse gastruloid model system to variations in N2B27 media formulations, highlighting the need for careful media s...

I do like the @dev-journal.bsky.social "Research Report" format for shortish papers

This one is valuable. "Is it the N2B27?" is the stem cell biologist version of "Is it the magnesium?"

Congrats Tina Balayo @dias-andre.bsky.social David Turner & all authors
journals.biologists.com/dev/article/...

MRC DiMeN Doctoral Training Partnership: How does Wnt and Notch (Wntch) signalling coordinate cell fate assignment during gastrulation? at University of Liverpool on FindAPhD.com PhD Project - MRC DiMeN Doctoral Training Partnership: How does Wnt and Notch (Wntch) signalling coordinate cell fate assignment during gastrulation? at University of Liverpool, listed on FindAPhD.com

Like Wnt signalling? How about Notch? How about both together (Wntch!)... in gastruloids? Come and and apply for a PhD studentship with us in Liverpool! Applications open 30th October! tinyurl.com/3bpx3y2b

This year's Crick @crick.ac.uk PhD programme is open for applications

- £27,715 tax-free student stipend per year for 4 years
- Tuition fees paid
- Student visa fee & health surcharges covered

Deadline 5 Nov

We have a project available. For all the details visit:

www.crick.ac.uk/careers-stud...

Figure 4 WT1 is expressed in the primitive streak, emerging mesoderm, neuromesodermal progenitor cells and LPM in E8.5 mouse embryos

New insights directly link WT1 and smooth muscle cell lineages

Read this Research Highlight showcasing work from Suad Alsukari, Huei Teng Ng, @gastruloids.bsky.social, Bettina Wilm @bwilm.bsky.social and colleagues:
journals.biologists.com/dev/article/...

Dynamic WT1 expression during gastrulation specifies peritoneal smooth muscle fate independently of mesothelial fate Highlighted Article: Genetic lineage tracing and expression analyses reveal insights into the developmental lineage of smooth muscle cells in the intestine and mesentery, specified by WT1 expression d...

Very happy to announce that this article is now published @dev-journal.bsky.social @biologists.bsky.social
journals.biologists.com/dev/article/...
With Suad Alsukari and Huei Teng Ng and @gastruloids.bsky.social

An englishman, down and out in Paris…..trying to read the news and laughing....at a french joke?
@gastruloids.bsky.social‬

Huge thanks to‬ @cci-liv.bsky.social for the amazing microscopes and technical support throughout this work!

Domain-specific AI segmentation of IMPDH2 rod/ring structures in mouse embryonic stem cells - BMC Biology Background Inosine monophosphate dehydrogenase 2 (IMPDH2) is an enzyme that catalyses the rate-limiting step of guanine nucleotides. In mouse embryonic stem cells (ESCs), IMPDH2 forms large multi-prot...

Look at this amount of work that has gone into the analysis of these beautiful (yes, we are biased) images of rods and rings. Now you can automatically classify these structures which is rather tedious to do manually. 🔬📷🖼️💻📄 bmcbiol.biomedcentral.com/articles/10....

I cannot help but think of these rod-rings as oh so pretty. amazing work by some of our @cci-liv.bsky.social users.

Great preprint out from the MicroAge team in
@livuni-ilcams.bsky.social! www.biorxiv.org/content/10.1...

Excellent work supporting #modularity of the mammalian #embryo, a role for #hypoxia & how development exploits these features. It shows what we can learn from assemblies of #gastruloids @ spatio-temporal coordination of the modules in AP organization. Putting modules together, extremely informative

The modularity of the mammalian #embryo in display. Controlled assembly of classic #gastruloids and uncommitted precursors in #hypoxia, patterns the AP axis
www.biorxiv.org/content/10.1...
Beautiful & insightful from @bulutkarslioglu.bsky.social‬ group. Building models to learn and learning.

This was a fabulous collaboration! Thanks @gastruloids.bsky.social and to Suad and Huei who worked incredibly hard to make it happen!

Also, an interesting reflection from Bob Edwards on the contrast between Research Institutes and Universities. Having spent my academic life in the latter, I can relate. From "A matter of life" a first hand account of the path to IVF that I suggest as a read for those interested in human #DevBio

Dynamic WT1 expression during gastrulation specifies peritoneal smooth muscle fate independently from mesothelial fate The Wilms’ Tumour protein (WT1) was previously linked to the mesothelial and vascular smooth muscle cell (vSMC) lineage in the mouse intestine, with evidence suggesting that intestinal vSMCs arise fro...

Great to see our article published in @dev-journal.bsky.social! Wonderfull collaboration with @bwilm.bsky.social to understand how Wt1 guides cell fate in the early gastrulating embryo! journals.biologists.com/dev/article/...

A time-resolved, multi-symbol molecular recorder via sequential genome editing - Nature A DNA memory device, DNA Typewriter, uses sequential prime editing to record the order of multiple cellular events.

We previously showed that DNA Typewriter can record high-resolution lineage trees in cultured cells (HEK293T). www.nature.com/articles/s41... But could we use it to study how clonal memory shapes cell fate decisions during development? (2/13)

Lineage recording in monoclonal gastruloids reveals heritable modes of early development Mammalian stem cells possess a remarkable capacity for self-organization, a property that underlies increasingly sophisticated in vitro models of early development. However, even under carefully contr...

New preprint with the Shendure lab @jshendure.bsky.social ! We apply the DNA Typewriter lineage recorder to mouse 3D gastruloids, a powerful model of early mammalian development. Led by Sam Regalado and CX Qiu @cxqiu.bsky.social : www.biorxiv.org/content/10.1... (1/13)

We've just updated our manuscript to a more 'publishable' version!
www.biorxiv.org/content/10.1...
New details about the role of Wnt in suppressing neural differentiation and fostering a posterior epiblast state, and the temporal effect of Nodal signalling in driving mesendodermal fates

Domain-specific AI segmentation of IMPDH2 rod/ring structures in mouse embryonic stem cells - BMC Biology Background Inosine monophosphate dehydrogenase 2 (IMPDH2) is an enzyme that catalyses the rate-limiting step of guanine nucleotides. In mouse embryonic stem cells (ESCs), IMPDH2 forms large multi-prot...

Our new paper just arrived! Fun collaborative effort led by Sam Ball and Meagan Hennessy to develop an AI tool to segment and classify #IMPDH2 rod-rings in mouse ESCs doi.org/10.1186/s129...

Gala dinner of the Biologists @ 100 conference at St Georges Hall, Liverpool.

Great fun at last night's Gala Dinner at the stunning
St Georges Hall.
#biologists100

Right after the spanish edition of #TheMasterBuilder comes the Portuguese one, thanks to www.temasedebates.pt
Have a look for a cell’s eye view of Biology in contrast with the widespread gene’s eye.
#CellsRUs #NotInTheGenes

Wonderful evening in #Barcelona w/ friends sharing memories, science and hope in these troubled times.

In the wake of Barcelona #Gastruloid meeting, we can say w/ some confidence that this is a system w/ potential in several areas of biomedical research: models, rather than replicas, are useful &also teach. For details
linkinghub.elsevier.com/retrieve/pii...
onlinelibrary.wiley.com/doi/10.1002/...

Compound design of a patient-derived 3D cell culture system modelling early peritoneal endometriosis Peritoneal endometriosis causes pelvic pain and infertility, but the underlying mechanisms related to these symptoms are not fully understood. Endometriosis diagnosis is typically delayed; thus, patie...

Delighted to announce our preprint on modelling of the peritoneum in endometriosis research, by Dimas Rahmana, with Dharani Hapangama's group, Liverpool Women's Hospital, with essential contribution from Chris Hill
www.biorxiv.org/content/10.1...
@livunihls.bsky.social @liverpooluni.bsky.social

First day of our Gastruloid Meeting in Barcelona kicking off with amazing talks and discussion and a room filled with gastruloid enthusiasts! #gastruloids #conference