Bratislav Dačević

📢📢 New paper out!

AI and networks can be used to predict distal mutations in proteins, even in artificial enzymes!
Check out the article here
----‐> doi.org/10.1016/j.ij...

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Engineering fluoroacetate dehalogenase by growth-based selections to degrade non-natural organofluorides The widespread use of organofluorides in modern society has inadvertently led to the bioaccumulation of harmful pollutants, most prominently per- and polyfluorinated alkyl substances (PFAS). In princi...

With #PFAS and other organofluorides wreaking havoc, we set out to engineer natural defluorinases for man-made organofluorides. All credit goes to the amazing @suzannejansen.bsky.social, who has outdone herself in this project! (1/5)
www.biorxiv.org/content/10.1...

#RepArtZymes pre-conference in Basel also brought together some of our old and current group members.
@zhizhou1619.bsky.social @reubenlg.bsky.social

Roelfes group - Open Positions PhD position on Hybrid biocatalytic cascades aided by Machine learning (1.0 FTE)

📢📢📢 PhD Opportunity!

Join our research group at RUG! We're seeking a motivated PhD student for a project on biocatalytic cascade processes combining natural and designer enzymes, supported by machine learning.
Click below to learn more — and feel free to share!

sites.google.com/rug.nl/roelf...

Artificial Metalloenzymes, Computational Chemistry, Polymer Chemistry, and Bioprocessing & Engineering, with inspiring guest speakers—all while surrounded by the stunning scenery of the Alps!

Had an amazing week at the MetRaZymes @metrazymes.bsky.social Winter School in Hirschegg, Austria! It was a pleasure to learn, share, and connect with fellow researchers in our doctoral network. We organized and attended insightful lectures and workshops on ⬇️🧵

Our latest publication is out in @angewandtechemie.bsky.social!

We generated and engineered a novel artificial metalloenzyme for the enantioselective synthesis of a panel of γ-nitro-carbonyl compounds on a preparative scale.

Check out our latest preprint, in which we demonstrate the first example of using noncanonical 3-aminotyrosine (aY) as a catalytic residue for iminium activation in a designer enzyme!

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Season's greetings from Roelfes and Mayer groups! Wishing our colleagues, collaborators, and the entire community a joyful holiday season and a prosperous New Year ahead! 🎉🎄✨🧪

OrgChem Starter pack has reached the 150 limit, so we started the sequel OrgChem2
go.bsky.app/GwH8tnF

Our OrgChem Starter Pack is growing with already 122 entries, but we will start a volume 2 if we go over the 150 limit!
go.bsky.app/NSEFPFJ

We are thrilled to share our new noble-metal enzyme design approach. We used the in-vivo incorporation of a noble-metal-binding ligand for ArM design to bridge the versatility of noble metals with the catalytic power of enzymes.
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We are delighted to introduce our new Boron Designer Enzyme for the kinetic resolution of α-hydroxythioesters. For this activity, the noncanonical amino acid and a natural lysine residue have to work together to form a Hybrid Catalytic Dyad!