King Cada

An efficient eukaryotic cell-free expression and correlative cryo-electron tomography platform for structural biology of macromolecular complexes www.biorxiv.org/content/10.1101/2025.10.18.683253v1 #cryoEM

Visualization of lysosomal membrane proteins by cryo electron tomography pubmed.ncbi.nlm.nih.gov/41107240/ #cryoEM

“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling Synaptic vesicle (SV) exocytosis underpins neuronal communication, yet its nanoscale dynamics remain poorly understood owing to limitations in visualizing rapid events in situ. Here, we used optogenet...

As ever, beautiful #cryoEM work from the lab of Hong Zhou with field-defining insights using #cryoET:

“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science

www.science.org/doi/10.1126/...

Structural basis of T-loop–independent recognition and activation of CDKs by the CDK-activating kinase Cyclin-dependent kinases (CDKs) are prototypical regulators of the cell cycle. The CDK-activating kinase (CAK) acts as a master regulator of CDK activity by catalyzing the activating phosphorylation o...

Now out in Science! Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. In @vcushing.bsky.social's magnum opus, we use #cryoEM to figure out how the CDK-activating kinase recognises CDKs to fully activate them - a key step in cell cycle control.
www.science.org/doi/10.1126/...

🧠 The Lipid #Brain Atlas is out now! If you think #lipids are boring and membranes are all the same, prepare to be surprised. Led by @lucafusarbassini.bsky.social with Giovanni D'Angelo's lab, we mapped membrane lipids in the mouse brain at high resolution.
www.biorxiv.org/cgi/content/...

Beautiful images @christlet.bsky.social !

See you there! Will try to throw a cytoskeleton party for my birthday 🎇

Beautiful live-cell imaging of lysosome tubulation along microtubules in this work just out from @lysoluis.bsky.social et al:
rupress.org/jcb/article/...

Cryo-ET structure of the microtubule-associated autoinhibited LRRK2^I2020T.

Using cryo-ET, CRN Team Reck-Peterson showed that full-length LRRK2 can oligomerize on microtubules in its autoinhibited state. Both WT-LRRK2 and PD-linked LRRK2 mutants formed filaments on microtubules.

🔗 Read the full #preprint to learn more about their recent research: bit.ly/3VrVNaB

Our new preprint is up! This is the main postdoc work of @wiesner-t.bsky.social focusing on exocytosis along the axon shaft and its regulation by the sub membrane actin-spectrin scaffold: www.biorxiv.org/content/10.1...
Read the thread below for a summary of our findings 🧵1/11

Woot! Congrats Chen!

Structural basis for mTORC1 activation on the lysosomal membrane pubmed.ncbi.nlm.nih.gov/40963021/ #cryoEM

Great couple of days working with colleagues at NIEHS and Duke!

Congrats!

Congrats James! Well deserved recognition

Reconstitution of autophagic-like membrane tethering reveals that Atg11 can bind and cluster vesicles on cargo mimetics Macroautophagy (hereafter, autophagy) is essential for the degradation of mitochondria from yeast to humans. Mitochondrial autophagy in yeast is initiated when the selective autophagy scaffolding p...

I'm very excited to share out latest work now published in Autophagy.

www.tandfonline.com/doi/full/10....

This work was led by Dr. Devika Andhare and demonstrates the membrane binding and clustering capabilities of Atg11 using a new reconstitution system.

Excited to share our new review with @depaceraffa.bsky.social‬, @saikat2025.bsky.social‬, and Chad Williamson on the BLOC-1 and BORC complexes—key regulators of endolysosomal processes and linked to several genetic diseases. #NIH #Lysosomes #RareDiseases authors.elsevier.com/a/1lg4i8jWWJ...

👉 New publication ahead of print from our lab: tinyurl.com/ye7pntte
We show that the protein SPG21, mutated in hereditary spastic paraplegia 21, localizes to endolysosomes via RAB7A, where it promotes mTORC1-dependent TFEB phosphorylation, reducing expression of a subset of TFEB regulated genes

Quantitative imaging of lipid transport in mammalian cells Nature - Directional, non-vesicular lipid transport is responsible for fast, species-selective lipid sorting into organelle membranes.

big one! a decade in the making. nadler & team develop tools to track lipid transport in living cells at single-species resolution. turns out non-vesicular, asymmetry-driven transport is the main architect of organelle identity. feels like the dawn of a golden era for lipid biology. rdcu.be/eBGJv

In situ cryo-electron tomography of vaccinia virus exit from infected cells https://www.biorxiv.org/content/10.1101/2025.08.20.671218v1

Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug Nature Structural & Molecular Biology - Integrated structural biology approach leveraging in situ cryo-electron tomography reveals molecular details of the native malarial translation...

Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH

A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo

Repost appreciated:
Open #PhDposition in structural virology in my group! Join us in Umeå, Sweden to uncover how arboviruses remodel infected cells. In situ #cryoET and #cryoEM combined with #virology, #cellbiology, and #biophysics.
Deadline 7 September. More info: www.carlsonlab.se/join/

So the cell is just one huge lysosome. Got it.

Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis Ferroptosis, a regulated form of cell death driven by excessive lipid peroxidation, has emerged as a promising therapeutic target in cancer. Ferroptosis suppressor protein 1 (FSP1) is a critical regul...

Excited to share our new preprint, led by Kirandeep Deol! 🤩 Genetic screens uncover mechanisms regulating FSP1 abundance in cancer. Vitamin B2 metabolism promotes FSP1 stability via FAD synth / binding, further linking nutrient metabolism to ferroptosis.
www.biorxiv.org/content/10.1...

Want to share your work at the premiere meeting of the minds between basic membrane biology and therapeutics? Short talk deadline is coming up Oct. 8, 2025. www.keystonesymposia.org/conferences/...

Congratulations @kev-in-situ-et.bsky.social and the whole team on in situ cryo-ET images of OA-induced mitochondria damage and Parkin-dependent mitophagy out now tinyurl.com/25b82cp4. We couldn't have done it without in situ guru @wilflinglab.bsky.social and mito guru @samlewis.bsky.social .‬

Thrilled to share a new preprint! 📝 🎉 Lewis lab PhD student Casadora wondered: Do mtDNA synthesis and lipid droplet biogenesis co-occur at the same membrane contact sites? Or can we discern distinct ER-mito contact classes? Evidence suggests the latter, relevant in overnutrition!

A bridge-like lipid transfer protein is critical for generation of invasive stages in malaria parasites https://www.biorxiv.org/content/10.1101/2025.07.25.666630v1

Wow! Congrats to everyone!

Your sleep pressure is powered by your mitochondria! Sleep, energy, and ageing are all connected through mitochondrial bioenergetics in neurons. Brilliant work from Gero Miesenböck’s lab, just out in Nature @nature.com
www.nature.com/articles/s41...