Daryan Chitsaz

Myoblasts labelled with phalloidin, embryonic rodent ventral spinal cord neurons with beta(III) tubulin, nuclei with DAPI.

In my MSc I co-cultured motor neurons (yellow) with muscle cells (red) in a separate compartment. One time the barrier between them broke and the chunk of neural tissue was swept up in a wave of proliferating myoblasts. The result reminds me of a boat in a tumultuous ocean storm. #fluorescenceFriday

8 years ago as a bumbling RA I tried to impress the cute French intern in the neighbouring lab by sculpting a spinal cord explant into a heart. The neurons did not fare particularly well and labelling leaves something to be desired, but we're still happily together 😍 #fluorescenceFriday #valentines

Live oligodendrocytes cultured on poly-L-lactic acid electrospun fibers, stained with the fluorescent lipid dye Fluoroymelin Red (which weakly labels the fibers, and strongly labels the cell membranes).

Oligodendrocytes normally make myelin by ensheathing neuronal axons, but these cells are so hell-bent on myelinating that they will wrap up anything, even tiny plastic fibers strung across a cell culture dish. These microfiber cultures let us rapidly screen for myelin-promoting drugs, and look cool.

Happy #fluorescenceFriday! This pic shows a gaggle of astrocytes with GFAP labelled, a major cytoskeletal protein specific to these cells. Astrocytes keep your neurons happy and well-fed, and have many other roles including regulating brain immune responses and maintaining the blood-brain barrier.

Wooo congrats, paper looks awesome! Very nice figures also, thanks for using colourblind-friendly colours

BEAT-MS | For people with relapsing multiple sclerosis A clinical study for relapsing multiple sclerosis

It would be nice to link to an article about the treatment, or at least a more legitimate source website. These women received hematopoietic stem cell transplants, a treatment that is currently in phase II clinical trials, such as this study which is still recruiting participants: www.beat-ms.org

Marmoset coronal sections. Left labelling is Hoechst (blue), Ferritin (green), and Fluoromyelin Red (red); right is MAP2 (blue), Tuj1 (green), and MBP (red). Montages stitched from ~200 tiles, thx imageJ.

For #fluorescenceFriday I'm celebrating a paper that got accepted this week on immunofluorescent brain slice labelling in Marmosets! These new-world primates are increasingly being used as research models for Austism spectrum disorder, but notably have not yet been reported Sieg Heil-ing.

This chaotic scene shows microglia (green), nimble little custodian-like brain cells, navigating through a dense canopy of myelin (red). Microglia support their communities, dissolve toxic particles, and don't threaten the sovereignty of friendly nations. Be more like microglia. #fluorescenceFridays

Hoecsht (blue), phalloidin (green), and beta (II) tubulin (red) on 2-day old murine dorsal root ganglia, which seems to have had some meninges or something attached (hence the fibroblast-y cells on the left)

Happy New Year y'all, here's a firework-ish pic of neurons "exploding" from a chunk of ganglia. Fiboblasts (green) bursting out of the left side provide an attractive substrate for sensory neuron axons (red), while other axons at the bottom grow in silly looping circles. #FluorescenceFriday #SciArt

This cool technique is called SCoRE (spectral confocal reflectance microscopy) first published by Schain et al in 2015 (https://doi.org/10.1038/nm.3495)

Myelin consists of many miniscule layers of smooth membrane, making it highly reflective. This lets you image it with #microscopy without any staining - left shows reflections of RGB lasers from brainstem myelin lit up like a disco ball, right is the same region with a fluorescent dye. #neuroskyence

Skimmed his papers and the RNAi study did just claim to reduce antigenicity not eliminate it, but still very cool research, thanks for sharing. Sorry my reply was a bit hostile, there's a ton of misinformation around GF diets and it gets aggravating!

Sadly some juice manufacturers add barley malt based sweeteners to juice so they can label it as having "natural flavours" instead of just adding sugar.

This sounds like bullshit, gluten is a major structural protein in wheat. RNA silencing can only dial down protein levels, not change their structure to make them less immunogenic. Perhaps "some guy" could use gene editing could make a safe form of wheat, celiacs can dream...

After a long day of live cell imaging this impressive specimen of an oligodendrocyte is heading for the sweet embrace of apoptosis. Happy weekend y'all #FluorescenceFriday #microscopy #cellbiology

We've seen an enormous amount of articles about upset businesses and CP customers already, how about a story on how postal worker salaries have fallen over the decades and the precarious position this puts them and their families in (all while executive pay has increased I'm sure)?

Explanted neural tissue can make for a dramatic image. This 2-day old chunk of dorsal root ganglia contains the sensory neurons (red) that allow you to feel. They don't particularly like growing on the glass plate, preferring to hop along hardy epithelial cells (green). #microscopy #neuroskyence

For #fluorescenceFriday sharing some sweet-ass actin: "birth of an endothelial cell" #cellbiology #microscopy

phd oral defense in about 24 hours, feeling like this stressed out little glial cell being treated with LPS #academiasky

As far as we can tell, covid doesn't affect these myelin producing cells directly (though other viruses like Epstein-barr can!).

Here's a weird type of microscopy - interference reflection microscopy (IRM)! Oligodendrocyte membranes are so smooth and reflective, you can see them without needing a dye by just bouncing laser light off of them (shown in blue, with cell body and processes in red). #neuroskyence
#microscopyMonday

Yes, these myelin-producing cells called Oligodendrocytes are targeted by the body's own immune system in MS. By studying how they develop and produce myelin, we hope to find new therapies to coax them to regenerate to stop and even repair the damage.

Thanks! The red is immunolabelled Myelin Basic Protein, a major component of and common marker for myelin sheaths. This was from a failed attempt at a new method for isolating the myelin producing cells, where most of the cells I ended up with were other types (probably astrocytes)

This slightly-botched tissue slice shows embryonic nervous system development. A blue DNA dye labels all cell nuclei, while neurons are green and sites where new ones are being produced are red. This pic was stitched from 100+ images, which is where that nasty grid comes from. #sciArt #neuroscience

These are a couple of glia (non-neuron brain cells). The lattice-like red stuff comes from a specialized cell that produces myelin, a substance that wraps around neurons to support them. This oligodendrocyte is hoping to find an axon to wrap but alas, there is none to be found. #SciArt #neuroscience

Suuper cool - how did you colour the images? Is it just a fire LUT?

I'm nearing the end of my neuroscience PhD and want to share some pics I took along the way.

Here are 2 cultured sensory neurons, the ones that carry information from your skin to spinal cord. These little guys only reach ~1/10th of a millimeter now, but can span several meters in large mammals.