Forneris Lab @UniPV

Summer holidays mood: on! - and a Great Birthday celebration: sushi lunch with all fellows and colleagues still active in the lab on August 7...

Enzymatic craftsmanship in collagen glycosylation - Nature Communications Researchers comment on two recent Nature Communications publications on enzyme complexes involved in collagen glycosylation, discussing how these findings lay a foundation for understanding collagen I...

Check the great commentary on @natcomms.nature.com from Trayambak Basak and colleagues about our work on #collagen GLT25D1 and the paper from Cao et al. about the LH3/GLT21D1 complex! #glycotime

www.nature.com/articles/s41...

Collagen IV Biosynthesis: Intracellular Choreography of Post-Translational Modifications Collagen IV, an essential and evolutionarily conserved component of basement membranes, is one of the most extensively post-translationally modified p…

Nothing better than seeing our latest review about #collageniv biosynthesis appearing online during the #collagen #GRC!

Super nice #dreamteamwork with @collagenyoshi.bsky.social, Rachel Lennon, Johanna Myllyharju, Antti Salo

www.sciencedirect.com/science/arti...

I also had fun in making gummy GLT25D1 making #notthecover using #blender

Molecular structure and enzymatic mechanism of the human collagen hydroxylysine galactosyltransferase GLT25D1/COLGALT1 - Nature Communications Hydroxylysine galactosylation is essential for collagen maturation. This work reveals the molecular structure of human GLT25D1, its catalytic residues, and a noncatalytic structural domain stabilized ...

Excited to share our new Nature Communications paper describing the molecular structure and the biochemical characterization of human #collagen #galactosyltransferase GLT25D1/COLGALT1 #glycotime www.nature.com/articles/s41...

Carne coltivata: 16 scienziati rispondono alla manifestazione di Coldiretti In una manifestazione promossa da Coldiretti, principale organizzazione degli imprenditori agricoli italiani, che si è svolta mercoledì 19 marzo a Parma, si sono radunate, secondo gli organizzatori, 2...

Coldiretti chiede per la carne coltivata procedure di autorizzazione equiparabili a quelle previste per i farmaci: 16 scienziati ed esperti che affermano che la richiesta non è basata su dati scientifici

www.scienzainrete.it/articolo/car...

Flyer for the 2025 Collagen Gordon Research Conference

Registration for the 2025 Collagen Gordon Research Conference is now open: www.grc.org/collagen-con...
Looking forward to seeing many of you there this summer! #CollagenGRC #TheMatrixIsEverywhere

The lecture room is really packed for this @thermofishersci.bsky.social #cryoem roadshow in Pavia today!

Extremely proud of our #postdoc Riccardo Piccinno, who got a young researchers' #grant from #FondazioneCariplo to develop a challenging #multidisciplinary project at #UniPV #DBB_unipv #insectbiology www.unipv.news/notizie/gran...

A packed lecture room full of #molecularbiology students following our friend and today's guest @iosonoirene #IITGenova lecture about Diving into the #3DGenome architecture @DBB_UniPV @unipv @ArmeniseHarvard

This year we joined @fondaz_inf_act to play games and discuss the science of #infectiousdiseases at @sharpernight @unipv @DBB_UniPV #PNRR @mur_gov_

Tomorrow... #ECM34 @fondaz_inf_act https://x.com/MFPronker/status/1827956491581567142

Amazing visit to the @harvardmed #CryoEM facility. @ArmeniseHarvard

A bit windy here in #Boston, ready to start with the #science program of @ArmeniseHarvard symposium about visualizing #biology at #nano and #macro scales!

Our graduate student @marcoberlinguer proudly showing his #CryoEM approaches to study #mosquito #biology at the dissemination event preceding #febs2024 @DBB_UniPV @unipv @mur_gov_ #PNRR @fondaz_inf_act

Molecular Structure and Enzymatic Mechanism of the Human Collagen Hydroxylysine Galactosyltransferase GLT25D1/COLGALT1 During biosynthesis, collagen lysine residues undergo extensive post-translational modifications essential for the stability and functions of collagen supramolecular assemblies. In the endoplasmic reticulum, two distinct metal ion dependent enzyme families (i.e., multifunctional lysyl hydroxylases-glucosyltransferases LH/PLODs and galactosyltransferases GLT25D/COLGALT) alternatively operate on collagen lysine side chains ultimately generating the highly conserved α-(1,2)-glucosyl-β-(1,O)-galactosyl-5-hydroxylysine pattern. Malfunctions in the collagen lysine post-translational modification machinery is linked to multiple developmental pathologies as well as extracellular matrix alterations causing enhanced cellular proliferation and invasiveness of several solid tumors, prompting for an in-depth characterization of LH/PLOD and GLT25D/COLGALT enzyme families. Here, we present an integrative molecular study of GLT25D1/COLGALT, highlighting an elongated head-to-head homodimeric assembly characterized by an N-terminal segment of each monomer wrapping around its dimerization partner. Each monomer encompasses two Rossman fold-type domains (GT1 and GT2) separated by an extended linker. Both domains were found capable of binding Mn2+ cofactors and UDP-α-galactose donor substrates, resulting in four candidate catalytic sites per dimer. Site-directed mutagenesis and biochemical studies identify the C-terminal GT2 domain as the functional GLT25D1/COLGALT1 catalytic site, highlighting an unprecedented Glu-Asp-Asp motif critical for metal ion binding, and suggesting structural roles for the N-terminal GT1 essential for correct quaternary structure assembly. Conversely, dimerization was not a requirement for GLT25D1/COLGALT1 enzymatic activity in vitro , suggesting that the elongated enzyme homodimer assembly, resembling that of LH/PLOD binding partners, could represent a functional hallmark for correct recognition and successful processing of collagen lysine residues. ### Competing Interest Statement The authors have declared no competing interest.

Excited to share our new @biorxivpreprint describing the integrative molecular characterization of GLT25D1/COLGALT1, a #collagen #glycosyltransferase with many unusual features: https://www.biorxiv.org/content/10.1101/2024.06.21.600124v1

Registration form for event 20 June 2024- 2pm BST (9am NY / 10am Canada / 3pm CEST)- on Zoom Title: Greening your lab; Sustainable Practices & Benefits for your Life Science Lab Speakers: Julie Di Martino, New York Medical College, USA & Federico Forneris, University of Pavia, Italy

In a couple of days! Join the discussion from #TheMeshwork about Sustainable Practices and Benefits for #LifeScience labs. Happy to be part of this panel! @unipv @DBB_UniPV #BioReMediA @AlbertaPinnola #PNRR

Registration link: https://t.co/LGHaJW9XLH https://t.co/L2TXlb5BL4

The call for #PhDpositions in Biomolecular Sciences and Biotechnology, jointly at @IussPavia and @unipv is open! DEADLINE July 15. Opportunities to join @MatteviLab @fornerislab @KajasteLab @SilviaBuroni @DBB_UniPV and many others!

https://t.co/GC9OcXvZof

Pleased to host once again our former postdoc @luigi_scietti #IEO to discuss #structuralvaccinology with @DBB_UniPV @unipv #molecularpharmacology #MBG students

Grateful to @THEmeshwork2020 for this opportunity to discuss some of our new ideas about #labsustainability @DBB_UniPV @unipv. Looking forward to sharing the online stage with @julieSdimartino!

#BioReMediA @AlbertaPinnola #PNRR @ecs_nodes #PoC @mur_gov_ https://t.co/L2TXlb5BL4

Pleased to host @MapelliMarina @LabMapelli #IEO giving a seminar about #celldivision #structuralbiology to #MBG #molecularpharmacology students and @DBB_UniPV @unipv colleagues @AIRC_it

Back again at the @esrfsynchrotron! #CryoEM #datacollection

Congratulations Piet!!! https://x.com/Bijvoet_Centre/status/1787438116250194237

Proud PI moment: our PhD student @JuliaDr32945512 defending her #PhD @IussPavia @unipv Biomolecular Sciences and Biotechnologies. Lots of nice data and questions questions from work done with @gab_paolo on #mosquito #saliva @DBB_UniPV @mur_gov_ @NATO_SPS

Yesterday at @unipv we hosted visitors exploring our molecular and cellular imaging laboratories, in the framework of #CRUI and @mur_gov_ initiative #UniversitaSvelate. A great experience to show our technologies and approaches!

Great to talk #mosquito #science at the @fondaz_inf_act RN2 meeting at #UNIBA #PNRR @DBB_UniPV @unipv

Hey #plantscientists #molecular #biologist, there's some good #research opportunities at @DBB_UniPV! https://x.com/AlbertaPinnola/status/1754564265397178392

It's the 10th anniversary of our lab! A #AcademicTwitter thread of highlights to celebrate http://fornerislab.unipv.it:

Collecting #feedback is essential during coordination of a large consortium. At the end of the 1NF-ACT meeting at @istsupsan, scientific and organizational directors of @fondaz_inf_act discuss with the #PNRR reviewing board for implementation of the next steps #infectiousdiseases