Lucas Cheadle

Thank you!

Figure 1 - Experimental design and introduction to the single-nucleus RNA sequencing dataset. (A) Schematic illustrating the late dark-rearing (LDR) paradigm and the workflow of the single-nucleus RNA sequencing (snRNAseq) experiments. (B) Quantification of Fos mRNA expression in sensory deprived (LDR) mice and in mice acutely exposed to light for between 15 min and 2 h, with stimulation timepoints labeled as follows: LDR15m (15 min of light), LDR30m (30 min), LDR1h (1 h) and LDR2h (2 h). Fos expression assessed by qPCR and normalized to Gapdh. Values plotted are additionally normalized to the LDR condition. (C) qPCR quantification of Jun mRNA expression (normalized to Gapdh) in V1 across all timepoints. Data obtained by qPCR and values plotted are normalized to LDR. In B and C, data are mean±s.e.m. n=3 mice per condition. One-way ANOVA followed by Tukey's post hoc test: *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. (D) Example confocal images of V1 in sections from a sensory deprived mouse (LDR) and a mouse re-exposed to light for 30 min (LDR30m). Fos mRNA (red), Jun mRNA (green) and DAPI (blue). Scale bar: 44 µm. (E) UMAP plot illustrating the 118,529 nuclei in the dataset categorized by general cell class: excitatory neurons (blue), inhibitory neurons (pink) and glia (green). (F) UMAP plot with all 16 clusters colored and labeled by cell type.

A single-cell transcriptomic atlas of sensory-dependent gene expression in developing mouse visual cortex

Read this this #LifelongDevSI #OA Techniques and Resources Article by Andre M. Xavier, Qianyu Lin, Chris J. Kang, Lucas Cheadle (@cheadlelab.bsky.social)

doi.org/10.1242/dev....

Thrilled to welcome the new FHS cohort… including these newly selected McKnight Scholars!

That’s my plan!

Wish I had known you were around!

Love this

Hosting Dr. Kaf Dzirasa @hhmi.org for a seminar at CSHL and getting to spend time w/fellow FHS @ishmailsaboor.bsky.social in the process has made this week a highlight of my year. Feeling seriously inspired today.

A single-cell transcriptomic atlas of sensory-dependent gene expression in developing mouse visual cortex Summary: Single-cell RNA sequencing of developing mouse visual cortex uncovers cell type-specific gene programs induced by sensory experience. All cell types mounted transcriptional responses to the e...

journals.biologists.com/dev/article/...

New work from the lab characterizing sensory-dependent transcriptomes in mouse V1 across neuronal and glial cell types. Some fun nuggets in here, and all data publicly available for download and re-analysis. Happy gene hunting!

Congrats!

Awesome to see this!

I’m curious whether the men were mentored to say “no” while the women were mentored to say “yes”. My mentors have always taught me to embrace saying “no” but maybe this advice differs by gender?

Delighted to share the published version of this work from brilliant #CancerNeuroscience post-doc Tara Barron detailing GABAergic neuron-to-glioma synapses in #DIPG/#DMG. 🧵
Open access link:
www.nature.com/articles/s41...

Dear friends and colleagues: nice to see you here where the skies are bluer. Follow me for updates on our research into neuro-immune interactions in brain development, function, and disease. @cshlnews.bsky.social @hhmi.bsky.social