Alexandria Shaw

Development of an inhibitory TTC7B selective nanobody that blocks EFR3 recruitment of PI4KA Phosphatidylinositol 4 kinase IIIα (PI4KIIIα/PI4KA) is an essential lipid kinase that plays a critical role in regulating plasma membrane identity. PI4KA is primarily recruited to the plasma membrane ...

More structural biology! From the @jburkevic.bsky.social lab with work from @sushantsuresh.bsky.social and @alexlouiseshaw.bsky.social - featuring data collected in our facility! #cryoEM
www.jbc.org/article/S002...

Excited to share our latest @nature.com: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇 www.nature.com/articles/s41...

Development of an inhibitory TTC7B selective nanobody that blocks EFR3 recruitment of PI4KA pubmed.ncbi.nlm.nih.gov/41197736/ #cryoEM

Sushant and Damilola in my group were running the BLI instrument nonstop to generate all of these curves. Alex was able to solve the cryo EM structure of this nanobody bound to PI4KA-TTC7B

Huge shout out to @hrmem.bsky.social - the cryo-EM work wouldn’t have been possible without them!

Cool new paper (www.jbc.org/article/S002... from my lab spearheaded by graduate students Sushant @sushantsuresh.bsky.social and Alex @alexlouiseshaw.bsky.social . This was a very cool collaboration with the Scott Hansen, Jennifer Cobb, Tamas Balla labs

Structure of calcineurin bound to PI4KA reveals dual interface in both PI4KA and FAM126A Phosphatidylinositol 4-kinase alpha (PI4KA) maintains the phosphatidylinositol 4-phosphate (PI4P) and phosphatidylserine pools of the plasma membrane.…

HRMEM is happy to spotlight this structure of calcineurin bound to PI4KA determined by Alexandria Shaw and colleagues from the Burke Lab at the University of Victoria and the Yip Lab at UBC!

We are proud to have provided the electron microscopy for this work!

Read more:
doi.org/10.1016/j.st...

Molecular basis for regulation of the class I phosphoinositide 3-kinases (PI3Ks), and their targeting in human disease The class I phosphoinositide 3-kinase pathway (PI3K) is a master regulator of cellular growth, and plays essential roles in controlling immune cell fu…

New review out from my lab on class I PI3Ks www.sciencedirect.com/science/arti..., was fun to write where we are and where we are going in PI3K research
Great work from Isobel and Alex in my lab @alexlouiseshaw.bsky.social

Cartoon of a scientist peering through a microscope saying "Whoaah!!!". Title says "The best part of Science is knowing, for a moment, something that nobody else in the world knows."

Really just had something like this feeling this afternoon. It’s the high that keeps you coming back for more

Great collaborative work where we developed a nanobody based tool to block EFR3 mediated recruitment of PI4KA. A potential therapeutic window maybe?
Thanks @alexlouiseshaw.bsky.social for the assist!

New preprint from the lab headed up by
@sushantsuresh.bsky.social! Late last year, we published the molecular basis of PI4KA plasma membrane recruitment by EFR3 (PMID:39705356). This work builds on these findings and identifies a new potential therapeutic avenue! Check it out!

New review out with @jburkevic.bsky.social!
We highlight recent advances in our understanding of the role the phosphoinositide PIP3 plays in regulating the protein kinases Akt, PDK1, and BTK from a structural perspective.
#kinase #phosphoinositides

portlandpress.com/biochemsoctr...

Reducing the effects of radiation damage in cryo-EM using liquid helium temperatures | PNAS The physical limit in determining the atomic structure of biological molecules is radiation damage. In electron cryomicroscopy, there have been num...

After years parked at a Polara, I’m excited to share that my PhD work is finally out! We figured out why imaging at liquid helium temperatures wasn’t working—and how to fix it.
🔗 www.pnas.org/doi/10.1073/...

An advanced toolset to manipulate and monitor subcellular phosphatidylinositol 3,5-bisphosphate In this paper, Pemberton et al. describe the generation and characterization of an engineered active fragment of the PIKfyve enzyme and its use to generate

New work out from my lab by my graduate student Isobel in collaboration with Josh Pemberton and Tamas Balla. Provides some new cool tools to manipulate PI3,5P2 levels, as well as some new insight into PIKfyve regulation.
rupress.org/jcb/article-...

An advanced toolset to manipulate and monitor subcellular phosphatidylinositol 3,5-bisphosphate. New study from Joshua Pemberton, John Burke, Tamas Balla (National Institute of Child Health and Human Development) and colleagues: rupress.org/jcb/article/...

#Biochemistry #CellSignaling #lipid

Back from vacation and really glad to share some work from my PhD research. Great collaborative effort @alexlouiseshaw.bsky.social Check out this post from my PI to learn more about Lipid kinase PI4KA and its regulation!

No better way to start the holidays then being able to share that some of my PhD research is live on Science Advances today!😄 A great collaborative effort! Check out this thread from my PI!

Could I be added to this please? Thanks! 😀