Nate Basisty (he/him)

Nice congrats! I was wondering what to call BlueSky tutorials

@bradolinger.bsky.social called it “blueprint” and I liked that…

Native, Spatiotemporal Profiling of the Global Human Regulome The regulome, comprising transcription factors, cofactors, chromatin remodelers, and other regulatory proteins, forms the core machinery by which cells interpret signals and execute gene expression pr...

Here we go, my first skeet-torial(?) on Bluesky for my first paper from @talusbio.bsky.social !

www.biorxiv.org/content/10.1...

This preprint describes our scalable, mass spec-based method for measuring which (and how many of them) proteins are interacting with DNA.

1/?

Thanks to all who contributed to the study! Including support from the NIA Intramural Research Program, NIH SenNet Common Fund Grant, and Hevolution Foundation. Thanks to the Seer team who were extremely responsive and helpful.

6/6

We hope this workflow enables other labs to perform comprehensive proteomic studies in mice, where it was not previously possible due to volume and dynamic range limitations.

#Proteomics #MassSpec #AgingResearch #Geroscience #Senescence #Biomarkers #Proteograph #bioRxiv #Olink #QEHF

5/n

We demonstrate the method's potential to detect aging /phenotype associated changes in serum. We observed completely different changes in blood at several ‘old’ ages, such as 24 months (old) and 30month (geriatric), reinforcing the importance of including multiple timepoints in aging studies

4/n

MS studies notably miss cytokines. A targeted mouse cytokine panel (O-link) on just 1 uL serum enabled us to quantify an additional 39 proteins not detected by MS (e.g. IL6), suggesting strong complimentary to the MS workflow. As expected, the majority of the cytokines increased with age.

3/n

Led by @bradolinger.bsky.social and Amit Dey – in 20uL serum we detected nearly 4000 proteins using the Proteograph workflow (4+ fold improvement) & demonstrated its consistency and accuracy w/ an 'LFQ-Bench-like' study. All on the Q-Exactive HF, so no need for the new fancy stuff.

2/n

Deep and Quantitative Proteomic Profiling of Low Volume Mouse Serum Across the Lifespan Assessing and validating circulating biomarkers is essential for the development of pre-clinical biomarkers that predict biological aging and aging-phenotypes in mice. However, comprehensive proteomic...

Where are the deep proteomic studies of mouse blood? We rarely see them because doing proteomics in mouse blood is challenging due to volume limitations and dynamic range.

I’m pleased to share our latest preprint showing a workflow that overcomes these challenges.

shorturl.at/hMpV6

1/n

We've learned a lot more about the principal drivers of age-related diseases in the past few weeks. And the leads to a unified model to put it all together. (open-access)
erictopol.substack.com/p/the-driver...

Check out the latest in aging proteomics from NIA at #ASMS2025! Please stop by and chat with our talented team! @bradolinger.bsky.social @reemabanarjee.bsky.social

Welcome to Baltimore, excited to have our mass spec colleagues and friends on our turf.

Don't Miss Out - TOMORROW - it's an interactive proteomics seminar series!

The CNPN x US HUPO Spotlight Series Webinar brings you cutting edge proteomics research from leading Canadian and US scientist.

Register NOW: ow.ly/lSY150VNhpI

Attending #ASMS2025 in Baltimore? Interested in quantitative proteomics? Join us Sunday Afternoon, June 1st for our annual Skyline User Group Meeting. We have a fantastic group of speakers. For information and to Register:
skyline.ms/project/home...
#massspectrometry #proteomics #metabolomics

#ASMS2025 is basically in my back yard this year!! Looking forward to reconnecting with friends and showcasing the latest from NIA after a bit of a break from conferences.

Happy to show off some fun neighborhoods (Fells, Fed Hill, etc) for #teammassspec- all pretty walkable. Hit me up!

Register Now! CNPN x US HUPO Spotlight Series seminar on cutting-edge proteomics research. Meet leading scientists from Canada and the US and learn how proteomics is changing the way we think about fungal disease and epigenetic mechanisms in human disease.

zoom.us/meeting/regi...

Check out our upcoming webinar! In this US HUPO X ABRF crossover event, you'll learn about where AI is currently used in proteomics and where it may be transformative in the future. Register now: ow.ly/tKB750VB1PN 🧪

Go Amanda !