Miguel Ramirez-Otero

New York millionaires: threatening to flee the city since 2009

Five ways Joe Rogan misleads listeners about climate change  » Yale Climate Connections Rogan exposes millions to climate denial. Let’s break down his tactics. 

Joe Rogan spent a whole podcast episode spouting climate misinformation. @dananuccitelli.bsky.social broke down the tactics Rogan and other climate deniers use so you can call them out when you hear them.

FIGNL1 inhibits homologous recombination in BRCA2 deficient cells by dissociating RAD51 filaments Homologous recombination (HR) deficiency upon Breast Cancer Gene 2 (BRCA2) loss arises from defects in the formation of RAD51 nucleoprotein filaments. We demonstrate that loss of the anti-recombinase ...

Want to know how homologous recombination defects are caused upon loss of BRCA2? Check out our recent efforts in uncovering this phenomenon just out in Science!
www.science.org/doi/10.1126/...

See our paper “Mechanism of trinucleotide repeat expansion by MutSβ-MutLγ and contraction by FAN1”. Using biochemistry, we show how DNA incisions by the MutLγ nuclease can lead to expansions, and how expansion is prevented by FAN1. Well done Issam and Valentina!

www.nature.com/articles/s41...

Spotify is running ICE ads. I went to cancel on mobile and saw that this isn’t allowed on mobile. They make you cancel on desktop. You think that’s gonna stop me, Spotify? I will erase you and forget you ever existed.

THOU SHALT CANCEL SPOTIFY!!!

A cytosolic function of DNMT1 controls neuronal morphogenesis via microtubule regulation Proteins traditionally confined to a single cellular compartment are increasingly recognized to exert non-canonical functions in alternative domains. The DNA methyltransferase 1 (DNMT1), classically d...

What if the DNA methylation enzyme DNMT1 isn’t just about DNA?
Our new preprint reveals a cytosolic role for DNMT1 as a scaffold controlling neuronal morphology via DOCK7–Rac1/STMN1 signaling & mitochondrial trafficking.
A paradigm shift in DNMT1 biology! www.biorxiv.org/content/10.1...

Distinct ATRX functions cooperate with 9-1-1 and CST complexes to safeguard replication and telomere integrity Mutations in the ATRX chromatin remodeller predispose to a developmental genetic disorder and cancer, but how it safeguards genome and telomere stability remains unresolved. Here, we uncover critical ...

🧬 Preprint alert!

"Distinct ATRX functions cooperate with 9-1-1 and CST complexes to safeguard replication and telomere integrity."

Read the manuscript here 👉 www.biorxiv.org/content/10.1...

EMBL-EBI and Google DeepMind renew partnership and release update to AlphaFold Database Deeper collaboration between EMBL-EBI and Google DeepMind brings updates to the AlphaFold Database.

@ewanbirney.bsky.social on the new version of Alphafold at the @emblebi.bsky.social
www.ebi.ac.uk/about/news/t...
AlphaFold has been a transformation not just in structural biology but also - due to its ability to be applied to all proteins - to molecular biology as a whole.

REVIEW: Modeling and targeting general and chromosome-specific aneuploidy in cancer.
By Aleah Goldberg, Maria Trifas and Teresa Davoli

Learn more here:
➡️ https://genesdev.cshlp.org/content/39/19-20/1132.abstract

RNF114 and RNF166 exemplify a new E3 ligases family that recognizes #MARUbylation –hybrid ADP-ribose/ubiquitin modifications– and extends them with K11-linked polyubiquitin
@jnpruneda.bsky.social @michaelnadbio.bsky.social and colleagues
www.embopress.org/doi/full/10....

Histone acetylation - not always about transcriptional activation: H4K16ac safeguards the genome replication program by repressing premature replication of heterochromatin. Well done Marta Milan! tinyurl.com/yuy9m8t3

Protein Domain Designer tool for the generation of publication-ready protein domain diagrams

New week, new tool: Find our Protein Domain Designer tool to generate publication-ready protein domain diagrams here: domaindesigner.farnunglab.com

2023 Cryo-EM Course - YouTube Held at the MRC Laboratory of Molecular Biology, the 2023 Cryo-EM Course offers insights into several aspects of electron cryo-microscopy. This includes disc...

This MRC course (and the references therein) is a great place to start. But it is a very broad topic - if you are looking to target e.g. membrane proteins, enzymes, RNA binding proteins you might get more specific sets of recommendations.

youtube.com/playlist?lis...

a painting of a girl with her eyes closed and the watermark tlouhq ALT: a painting of a girl with her eyes closed and the watermark tlouhq

This preprint title for the win! "The First of Us: Ophiocordyceps use a novel scramblase-binding peptide to manipulate zombie ants" doi.org/10.1101/2025...

Science is cool too! Authors use #celegans to identify the targets... #cilia and scramblases

The eukaryotic replisome intrinsically generates asymmetric daughter chromatin fibers DNA replication is molecularly asymmetric, due to distinct mechanisms for lagging and leading strand DNA synthesis. Whether chromatin assembly on newly replicated strands is also asymmetric remains un...

Some (+)ve news to lighten another heavy weekend: our latest preprint (c/o Mattiroli + Ramani labs) is up!
www.biorxiv.org/content/10.1...
A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)

Tariff update At Cytoskeleton, one of our top priorities is to maintain a fair price on high quality products. Unfortunately, tariffs have been implemented on many countries and one of them impacts a very popular line from Switzerland. Currently, that country is subject to 39% tariff on products imported to the USA since August 7th 2025. The main product line affected is Spirochrome's line of bioprobes for live cell work. Starting Monday 22nd September we will be adding a temporary tariff fee on all the Spirochrome items. Cytoskeleton is taking some of the tariff burden so we can reduce the customer tariff fee to 24% of list price which will be implemented for the duration of the tariff period. Its an unfortunate situation which we hope will return to normal asap. Sincerely, Team at Cytoskeleton Inc.

I'm more worried about the impact on my farmer neighbors but science is also affected by Trump's tariff insane policies

Biochemical reconstitution of sister chromatid cohesion establishment during DNA replication Genome inheritance during cell divisions relies on replicated sister DNAs being connected by cohesin rings. Minamino et al. reconstitute sister chromatid cohesion establishment during DNA replication using purified budding yeast proteins. The experiments yield molecular insight into more than one way in which sister chromatid cohesion can be built.

Online Now: Biochemical reconstitution of sister chromatid cohesion establishment during DNA replication Online now:

Is there anyone here at one of the universities in Wellington, NZ, who would want to host me to give a talk around November 10th?

SLFN11 restricts escape from telomere crisis to prevent alternative lengthening of telomeres The tRNA nuclease SLFN11 is epigenetically silenced in ~50% of treatment-naive tumours and is the strongest predictor of chemoresistance but why it is frequently inactivated in cancer is unknown. To a...

🚀 New preprint alert!

We uncover SLFN11 as a molecular gatekeeper that restricts alternative lengthening of telomeres (ALT) by sensing telomere-associated replication stress and triggering apoptosis.

📄 Read the manuscript here: www.biorxiv.org/content/10.1...

DNA2 enables growth by restricting recombination-restarted replication - Nature DNA2 suppresses recombination-restarted replication and checkpoint activation at stalled forks, and its loss triggers recombination-dependent synthesis, checkpoint signalling and cell-cycle exit, high...

#New-Paper Rass laboratory reveal DNA2 to be a gatekeeper to homologous recombination at stalled DNA replication forks, rrequired to stave off cellular senescence. Their findings provide a molecular explanation for the association of DNA2 with primordial dwarfism and cancer.

doi.org/10.1038/s415...

DNA2 enables growth by restricting recombination-restarted replication - Nature DNA2 suppresses recombination-restarted replication and checkpoint activation at stalled forks, and its loss triggers recombination-dependent synthesis, checkpoint signalling and cell-cycle exit, high...

My first post on bsky to share our latest research in @nature.com: “DNA2 enables growth by restricting recombination-restarted replication” - defines DNA2’s essential function & elucidates links with primordial dwarfism and cancer. Thanks to the lab, collaborators & @ukri.org @acmedsci.bsky.social

A split-site E3 ligase mechanism enables ZNFX1 to ubiquitinate and cluster single-stranded RNA into ubiquitin-coated nucleoprotein particles Grabarczyk et al. show the structure and mechanism of a non-canonical ubiquitin ligase, which is activated through nucleic-acid-induced oligomerization and is critical for cell survival during immune ...

Happy to share our work on the structure and function of the unusual E3 ligase ZNFX1 @cp-cell.bsky.social. It uses a nucleic acid-activated transthiolation mechanism, ubiquitinating and clustering RNA to protect cells in an immune response. @clausenlab.bsky.social
www.cell.com/cell/fulltex...

Of course I signed this! Please join us.

I am so disturbed by this image of ICE agents at Stanford hospital. They do not belong here--actually, they don't belong anywhere.

Highly specific intracellular ubiquitination of a small molecule - Nature Chemical Biology Li et al. discovered that the cytotoxic synthetic small molecule BRD1732 is directly ubiquitinated in cells. Ubiquitination of BRD1732 is E3 ligase dependent and leads to inhibition of proteasomal deg...

A new paper reports direct ubiquitination of a small molecule BRD1732 in cells. BR31732 ubiquitination is E3 ligase-dependent and leads to inhibition of proteasomal degradation.

www.nature.com/articles/s41...

Sometimes, knocking out a gene isn't a good way to figure out the function of that gene ...

In our new @jcellsci.bsky.social paper, we report potent transcriptional adaptation by cytotoxic T lymphocytes (CTLs) in response to CRISPR/Cas9-mediated deletion of the important polarity protein CDC42 🧵⬇️

Targeting G1–S-checkpoint-compromised cancers with cyclin A/B RxL inhibitors - Nature Dual cyclin A/B RxL inhibitors selectively kill small cell lung cancer cells and other cancer cells with high E2F activity.

new out in Nature
www.nature.com/articles/s41...

Excited to see this work published online at @cp-cell.bsky.social today!

www.cell.com/cell/fulltex...

Nitric oxide promotes cysteine N-degron proteolysis through control of oxygen availability | PNAS Selected proteins containing an N-terminal cysteine (Nt-Cys) are subjected to rapid, O2-dependent proteolysis via the Cys/Arg-branch of the N-degro...

Nitric oxide promotes cysteine N-degron proteolysis through control of oxygen availability | Important finding by Kim, Tian, Ratcliffe and Keeley @ox.ac.uk @ispt-proteinterm.bsky.social #N-degron www.pnas.org/doi/10.1073/...