Miguel Ramirez-Otero

REVIEW: Modeling and targeting general and chromosome-specific aneuploidy in cancer.
By Aleah Goldberg, Maria Trifas and Teresa Davoli

Learn more here:
➡️ https://genesdev.cshlp.org/content/39/19-20/1132.abstract

RNF114 and RNF166 exemplify a new E3 ligases family that recognizes #MARUbylation –hybrid ADP-ribose/ubiquitin modifications– and extends them with K11-linked polyubiquitin
@jnpruneda.bsky.social @michaelnadbio.bsky.social and colleagues
www.embopress.org/doi/full/10....

Histone acetylation - not always about transcriptional activation: H4K16ac safeguards the genome replication program by repressing premature replication of heterochromatin. Well done Marta Milan! tinyurl.com/yuy9m8t3

Protein Domain Designer tool for the generation of publication-ready protein domain diagrams

New week, new tool: Find our Protein Domain Designer tool to generate publication-ready protein domain diagrams here: domaindesigner.farnunglab.com

2023 Cryo-EM Course - YouTube Held at the MRC Laboratory of Molecular Biology, the 2023 Cryo-EM Course offers insights into several aspects of electron cryo-microscopy. This includes disc...

This MRC course (and the references therein) is a great place to start. But it is a very broad topic - if you are looking to target e.g. membrane proteins, enzymes, RNA binding proteins you might get more specific sets of recommendations.

youtube.com/playlist?lis...

a painting of a girl with her eyes closed and the watermark tlouhq ALT: a painting of a girl with her eyes closed and the watermark tlouhq

This preprint title for the win! "The First of Us: Ophiocordyceps use a novel scramblase-binding peptide to manipulate zombie ants" doi.org/10.1101/2025...

Science is cool too! Authors use #celegans to identify the targets... #cilia and scramblases

The eukaryotic replisome intrinsically generates asymmetric daughter chromatin fibers DNA replication is molecularly asymmetric, due to distinct mechanisms for lagging and leading strand DNA synthesis. Whether chromatin assembly on newly replicated strands is also asymmetric remains un...

Some (+)ve news to lighten another heavy weekend: our latest preprint (c/o Mattiroli + Ramani labs) is up!
www.biorxiv.org/content/10.1...
A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)

Tariff update At Cytoskeleton, one of our top priorities is to maintain a fair price on high quality products. Unfortunately, tariffs have been implemented on many countries and one of them impacts a very popular line from Switzerland. Currently, that country is subject to 39% tariff on products imported to the USA since August 7th 2025. The main product line affected is Spirochrome's line of bioprobes for live cell work. Starting Monday 22nd September we will be adding a temporary tariff fee on all the Spirochrome items. Cytoskeleton is taking some of the tariff burden so we can reduce the customer tariff fee to 24% of list price which will be implemented for the duration of the tariff period. Its an unfortunate situation which we hope will return to normal asap. Sincerely, Team at Cytoskeleton Inc.

I'm more worried about the impact on my farmer neighbors but science is also affected by Trump's tariff insane policies

Biochemical reconstitution of sister chromatid cohesion establishment during DNA replication Genome inheritance during cell divisions relies on replicated sister DNAs being connected by cohesin rings. Minamino et al. reconstitute sister chromatid cohesion establishment during DNA replication using purified budding yeast proteins. The experiments yield molecular insight into more than one way in which sister chromatid cohesion can be built.

Online Now: Biochemical reconstitution of sister chromatid cohesion establishment during DNA replication Online now:

Is there anyone here at one of the universities in Wellington, NZ, who would want to host me to give a talk around November 10th?

SLFN11 restricts escape from telomere crisis to prevent alternative lengthening of telomeres The tRNA nuclease SLFN11 is epigenetically silenced in ~50% of treatment-naive tumours and is the strongest predictor of chemoresistance but why it is frequently inactivated in cancer is unknown. To a...

🚀 New preprint alert!

We uncover SLFN11 as a molecular gatekeeper that restricts alternative lengthening of telomeres (ALT) by sensing telomere-associated replication stress and triggering apoptosis.

📄 Read the manuscript here: www.biorxiv.org/content/10.1...

DNA2 enables growth by restricting recombination-restarted replication - Nature DNA2 suppresses recombination-restarted replication and checkpoint activation at stalled forks, and its loss triggers recombination-dependent synthesis, checkpoint signalling and cell-cycle exit, high...

#New-Paper Rass laboratory reveal DNA2 to be a gatekeeper to homologous recombination at stalled DNA replication forks, rrequired to stave off cellular senescence. Their findings provide a molecular explanation for the association of DNA2 with primordial dwarfism and cancer.

doi.org/10.1038/s415...

DNA2 enables growth by restricting recombination-restarted replication - Nature DNA2 suppresses recombination-restarted replication and checkpoint activation at stalled forks, and its loss triggers recombination-dependent synthesis, checkpoint signalling and cell-cycle exit, high...

My first post on bsky to share our latest research in @nature.com: “DNA2 enables growth by restricting recombination-restarted replication” - defines DNA2’s essential function & elucidates links with primordial dwarfism and cancer. Thanks to the lab, collaborators & @ukri.org @acmedsci.bsky.social

A split-site E3 ligase mechanism enables ZNFX1 to ubiquitinate and cluster single-stranded RNA into ubiquitin-coated nucleoprotein particles Grabarczyk et al. show the structure and mechanism of a non-canonical ubiquitin ligase, which is activated through nucleic-acid-induced oligomerization and is critical for cell survival during immune ...

Happy to share our work on the structure and function of the unusual E3 ligase ZNFX1 @cp-cell.bsky.social. It uses a nucleic acid-activated transthiolation mechanism, ubiquitinating and clustering RNA to protect cells in an immune response. @clausenlab.bsky.social
www.cell.com/cell/fulltex...

Of course I signed this! Please join us.

I am so disturbed by this image of ICE agents at Stanford hospital. They do not belong here--actually, they don't belong anywhere.

Highly specific intracellular ubiquitination of a small molecule - Nature Chemical Biology Li et al. discovered that the cytotoxic synthetic small molecule BRD1732 is directly ubiquitinated in cells. Ubiquitination of BRD1732 is E3 ligase dependent and leads to inhibition of proteasomal deg...

A new paper reports direct ubiquitination of a small molecule BRD1732 in cells. BR31732 ubiquitination is E3 ligase-dependent and leads to inhibition of proteasomal degradation.

www.nature.com/articles/s41...

Sometimes, knocking out a gene isn't a good way to figure out the function of that gene ...

In our new @jcellsci.bsky.social paper, we report potent transcriptional adaptation by cytotoxic T lymphocytes (CTLs) in response to CRISPR/Cas9-mediated deletion of the important polarity protein CDC42 🧵⬇️

Targeting G1–S-checkpoint-compromised cancers with cyclin A/B RxL inhibitors - Nature Dual cyclin A/B RxL inhibitors selectively kill small cell lung cancer cells and other cancer cells with high E2F activity.

new out in Nature
www.nature.com/articles/s41...

Excited to see this work published online at @cp-cell.bsky.social today!

www.cell.com/cell/fulltex...

Nitric oxide promotes cysteine N-degron proteolysis through control of oxygen availability | PNAS Selected proteins containing an N-terminal cysteine (Nt-Cys) are subjected to rapid, O2-dependent proteolysis via the Cys/Arg-branch of the N-degro...

Nitric oxide promotes cysteine N-degron proteolysis through control of oxygen availability | Important finding by Kim, Tian, Ratcliffe and Keeley @ox.ac.uk @ispt-proteinterm.bsky.social #N-degron www.pnas.org/doi/10.1073/...

PhD student to study the mechanisms and therapeutic potential of genomic instability

Aspiring PhD students, this is your chance to join us at the NKI to work on genomic instability and telomeres!: www.werkenbijavl.nl/vacatures/ph...

NASP modulates histone turnover to drive PARP inhibitor resistance - Nature PARP inhibitor treatment triggers histone release from the chromatin in cancer cells; consequently, targeting the histone chaperone NASP renders cells vulnerable to PARP inhibition.

Thrilled to share our latest work, just published in @nature.com ⬇
www.nature.com/articles/s41...

We discovered that PARP inhibitors 💊 trigger histone eviction from the chromatin and this creates a hidden vulnerability in PARPi resistant tumors.
🧵 (1/8)

WORK!

I currently have 5 (!) open positions for postdocs/PhD students in our CDlab for projects:

- Nanopore protein sequencing
- Archaeal CDV cell division
- Microfluidics for synthetic cells
- Nuclear Pore Complex
- Origami mimics of peroxisomes

Please apply! ceesdekkerlab.nl/come-join-us/

RT=👍

Ubiquitination of the histone variant mH2A1.2 prevents toxic RAD18 accumulation at a subset of genomic loci upon replication stress Using biochemical assays and a mutant disrupting RNF168-dependent ubiquitination of the histone variant macroH2A1.2, Galloy et al. identified an unexpected role for histone mH2A1.2 ubiquitination in p...

I am very excited to share the newest paper of the lab, a huge amount of work led by our talented PhD student Maxime Galloy, in close collaboration with Andréanne Blondeau, our dedicated research assistant for 10 years! 🙌

www.cell.com/molecular-ce...

Biozentrum PhD Fellowships Share your passion for life sciences. If you are talented and highly motivated, want to broaden your horizons and are interested in a wide range of research topics, apply for one of the sought after B...

Apply now for the prestigious and independent #BiozentrumPhDFellowships. Great science. Unique rotation-based selection of research group and other incentives. The summer call is open until October 12, 2025. bit.ly/4caiqqX @biozentrum.unibas.ch @unibas.ch #fellowship #PhD#Switzerland

To have this paper appear the same day that RFK Jr is canceling all mRNA vaccine funding is further indication that irony is not dead, even though we might all be soon.

PhD student DNA repair in kidney cancer | Werken bij UMC Groningen

We have an open position for a PhD student to join our team. If you have an interest in cancer biology, genome maintenance, or both, please apply! Details can be found in the link below. With @marcelvanvugt.bsky.social.

werkenbijumcg.nl/-/vacatures/...

Snails with human-like eyes?!

Published in @natcomms.nature.com, the @planaria1.bsky.social & @accorsi-alice.bsky.social Labs have established apple snails as a novel model for studying vision restoration. They have complex camera-type eyes & the ability to regrow them in 28 days: bit.ly/4mvaxkq

🍂The 2025 Fall Session of GIIN webinars is here!
Join us on the 1st Tuesday of each month at 5 PM CET (Rome) / 11 AM DET (NYC). No pre-registration needed, just click to join via Zoom: uniroma1.zoom.us/j/95149984403
Subscribe here for webinar reminders: docs.google.com/forms/d/e/1F...